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Ion OneTouch 2 System - Thermo Fisher Scientific

For Research Use Only. Not for use in diagnostic OneTouch 2 SystemUSER GUIDEFor installation, setup, and maintenance of the Ion OneTouch 2 Instrument and Ion OneTouch ES InstrumentCatalog Number 4474779 Publication Number MAN0014388 Revision information in this guide is subject to change without THE EXTENT ALLOWED BY LAW, LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT,PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF Licensing InformationThese products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept the terms and conditions of allapplicable Limited Use Label entityLife Technologies | Carlsbad, CA 92008 USA | Toll Free in USA trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. Kimwipes is a trademark of Kimberly-Clark Corporation. Eppendorf and Eppendorf LoBind are trademarks of Eppendorf AG.

Ion OneTouch™ 2 System USER GUIDE ... • Ion OneTouch™ Sippers • Ion OneTouch™ 2 Amplification Plates See product web pages ... In the instrument touch screen, touch Options, then touch Info. Firmware updates can only be installed by Administrator-level users on the Torrent

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Transcription of Ion OneTouch 2 System - Thermo Fisher Scientific

1 For Research Use Only. Not for use in diagnostic OneTouch 2 SystemUSER GUIDEFor installation, setup, and maintenance of the Ion OneTouch 2 Instrument and Ion OneTouch ES InstrumentCatalog Number 4474779 Publication Number MAN0014388 Revision information in this guide is subject to change without THE EXTENT ALLOWED BY LAW, LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT,PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF Licensing InformationThese products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept the terms and conditions of allapplicable Limited Use Label entityLife Technologies | Carlsbad, CA 92008 USA | Toll Free in USA trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. Kimwipes is a trademark of Kimberly-Clark Corporation. Eppendorf and Eppendorf LoBind are trademarks of Eppendorf AG.

2 Luer-Lok is a trademark of Becton, Dickinson and is trademark of Bemis Company, Inc. Xiameter is a trademark of Dow Corning Corporation. Macintosh and Mac OS are trademarks of Apple,Inc. Microsoft, Windows, and Excel are trademarks of Microsoft Corporation. 2015 Thermo Fisher Scientific Inc. All rights this 6 Revision 6 Purpose of the 6 CHAPTER 1 Product 7 Instrument System and 7 Required materials and equipment (not provided).. 8 CHAPTER 2 Ion OneTouch 2 Instrument installation, setup,and and install the Ion OneTouch 2 and updating the firmware on the Ion OneTouch 2 the firmware a firmware update with a USB flash method: Install a firmware update using an ethernet OneTouch 2 Instrument touchscreen up the Ion OneTouch 2 Instrument ..15 Materials required for this the Ion OneTouch Recovery Tubes and Ion OneTouch Recovery the Ion OneTouch 2 Amplification Plate ..17 Install the disposable the Ion OneTouch the Ion OneTouch Recovery Solution.

3 19 Empty the Waste the oil waste the Ion OneTouch 2 Cleaning Adapter ..20 Initialize the the set a verification OneTouch 2 Instrument the Ion OneTouch 2 OneTouch 2 System user guide 3 Ion OneTouch 2 Instrument and equipment the the log files from the Ion OneTouch 2 CHAPTER 3 Ion OneTouch ES Instrument installation, setup,and and install the Ion OneTouch ES the AC line voltage fuse up the OneTouch ES required for this up the OneTouch ES Instrument the vertical the horizontal a residual volume OneTouch ES and equipment OneTouch ES back panel and lubricate the the OneTouch ES and equipment the CHAPTER 4 Set up and test the Ion Chip the Ion S5 /Ion Proton Rotor and the CHAPTER 5 Demonstrated protocol: Switching betweenolder and newer template preparation switching is materials and you the Ion OneTouch 2 Instrument after the last the Ion OneTouch 2 up the Ion OneTouch 2 Instrument with the appropriate OneTouch 2 System user guide APPENDIX A OneTouch 2 Instrument.

4 58 Ion OneTouch ES ..60 APPENDIX B on this symbols on this alerts on this of safety labels on the information for instruments not manufactured by Thermo Fisher and and electromagnetic compatibility (EMC) Documentation and and technical product OneTouch 2 System user guide 5 About this guideCAUTION! ABBREVIATED SAFETY ALERTS. Hazard symbols and hazardtypes specified in procedures may be abbreviated in this document. For thecomplete safety information, see the Safety appendix in this ! Before using this product, read and understand the information in the Safety appendix in this 2015 Instrument user guide that includes instructions forinstallation, setup, and of the guideThe Ion OneTouch 2 System user guide (Pub. no. MAN0014388) provides referenceinformation for the installation, setup, and maintenance of the Ion OneTouch 2 System (Cat. no. 4474779), which includes the Ion OneTouch 2 Instrument and theIon OneTouch ES Instrument.

5 For information about how to run these instruments,see the template kit-specific user OneTouch 2 System user GuideProduct informationInstrument System and componentsThe Ion OneTouch 2 System is an automated System used for the generation oftemplate-positive Ion Sphere Particles (ISPs) for semiconductor Ion OneTouch 2 System (Cat. no. 4474779) contains the following boxes andcomponents:DescriptionCatalog OneTouch 2 Instrument[1]4474778, 4474779 Ion OneTouch ES Instrument4474779AC Power Supply and Cords[1]Sold separately or as part of the : See the sequencer-specific site preparation guide for information on sitepreparation for the sequencer and the Ion OneTouch 2 : We recommend using an uninterruptable power supply (UPS) for laboratoriesthat experience frequent power outages or line voltage fluctuations. The UPS must berated for 1500 W output or higher. The 1500 VA unit from APC provides severalminutes of backup power for the Ion OneTouch 2 Instrument and Ion OneTouch ESInstrument.

6 Use a surge protector or line conditioner as OneTouch 2 System user guide 7 Required materials and equipment (not provided)Installation, setup, and maintenance of the Ion OneTouch 2 System uses commonmolecular biology equipment, supplies, and reagents. Unless otherwise indicated, allmaterials are available through MLS: Fisher Scientific ( ) or other major laboratory [1]SourceIon OneTouch template preparation solutions (provided in your Ion OneTouch Template Kit): Ion OneTouch Oil Ion OneTouch Recovery SolutionSee product webpagesIon OneTouch template preparation supplies (provided in your Ion OneTouch Template Kit): Ion OneTouch Reagent Tubes Ion OneTouch Recovery Routers Ion OneTouch Recovery Tubes Ion OneTouch Sippers Ion OneTouch 2 Amplification PlatesSee product webpagesOne of the following control library kits:For PGM Sequencers: E. coli DH10B Control Library from the Ion PGM Controls Kit v2 For Ion Proton Sequencers: Human CEPH Control 200 Library from the Ion PI Controls 200 KitFor Ion S5 Sequencers.

7 Human CEPH Control 200 Library from the Ion S5 Controls Kit 44820104488985A27760 Dynabeads MyOne Streptavidin C1 Magnetic Beads65001 DynaMag -2 magnet12321 DGeneAmp PCR System 9700 thermal cycler or equivalentN8050200 (Base)4314443 (Block)Category 6 Ethernet CableMLSS urge protector or line Eppendorf LoBind PCR tubesAxygenPCR-02-L-CBioExpressT-3035-1 Microcentrifuge[2]MLSP ipettors (P2, P20, P200, P1000) and appropriate low retention tipsMLSV ortexer with a rubber platformMLSTube rack to fit 15-mL conical tubeMLSTube rack for 50-mL conical tubeMLSC hapter 1 Product informationRequired materials and equipment (not provided)18 Ion OneTouch 2 System user GuideItem[1]Source1 M NaOHMLSB leachMLSX iameter PMX-200 Silicone Fluid[3]Neely IndustriesPMX200-12500 PTBenchtop absorbent paper or matMLS1/8-inch L-wrench (hex wrench) or equivalent toolMLS[1] We have verified this protocol using these specific materials. Substitution may adversely affect System performance.

8 [2]Must fit standard and microcentrifuge tubes; must generate 15,500 g .[3]Material required for periodic maintenance of the Ion OneTouch ES. Chapter 1 Product informationRequired materials and equipment (not provided)1 Ion OneTouch 2 System user guide 9 Ion OneTouch 2 Instrumentinstallation, setup, and maintenanceInstrument clearancesPosition the instrument so that the front is a minimum of 12 in. ( cm) from thefront of the laboratory bench. Place the instrument at least 40 in. (1 meter) away frommajor sources of electronic noise such as refrigerators or microwaves. For moreinformation, refer to the appropriate site preparation guide specific for yoursequencing and install the Ion OneTouch 2 InstrumentFor detailed instructions on site preparation and installation of the Ion OneTouch 2 Instrument, refer to the site preparation and installation requirements in yoursequencing System site preparation and install the Ion OneTouch 2 Instrument in a location different fromthe location used to prepare the amplification solution.

9 Remove the instrumentby laying the shipping box sideways on a table, then sliding out the ! Do not lift the instrument by the metal handle used to access theIon OneTouch 2 Amplification that the power switch is turned Off, then plug the power cord into the other end of the power cord into an electrical outlet of the ! The Ion OneTouch 2 Instrument draws 6 amps of current. Do notexceed the circuit breaker limit for current. If necessary, plug multipleinstruments into different the power switch On. Initial start-up takes ~3 minutes. You may hearsounds from the instrument. This is ! Leave the Ion OneTouch 2 Instrument On indefinitely. If theinstrument is turned off, critical log files are lost. If you need to turn off theinstrument or if a run fails, download the log files (see Download the log filesfrom the Ion OneTouch 2 Instrument on page 26).210 Ion OneTouch 2 System user GuideChecking and updating the firmware on the Ion OneTouch 2 InstrumentFirmware updates to the software controlling the Ion OneTouch 2 Instrument areperiodically released.

10 To update the firmware to the appropriate version, use either aUSB flash drive or an ethernet connection, as described in the next ! Ensure that the latest firmware is installed on the Ion OneTouch 2 Instrument. However, if you are using the Ion PGM Template OT2 200 Kit togetherwith the Ion PGM Hi-Q Sequencing Kit, do not upgrade to Torrent Suite or later until you no longer require the mixed workflow. The OT2 program scriptenabling the mixed workflow is discontinued in We recommend using the IonPGM Hi-Q OT2 Kit (Cat. no. A27739) together with the Ion PGM Hi-Q Sequencing the instrument touch screen, touch Options, then touch updates can only be installed by Administrator-level users on the TorrentServer connected to your Ion OneTouch 2 that the USB flash drive is FAT32-formatted. If your USB is not FAT32formatted, follow the instructions below to reformat : Reformatting will erase on the data on the OS 1. Insert the USB flash drive into a USB port, then double-click theMacintosh HDD Navigate to the Applications folder, then click: Double-click the Disk Utility Select the drive (the USB flash drive) to format, then From the volume format, select MS-DOS (FAT), then enter aname for the USB flash Click Erase, then confirm the FAT32 1.


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