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ITC Broth Base (TTC Broth Base) - HiMedia Labs

Please refer disclaimer Broth base (TTC Broth Base) M1220 ITC or TTC Broth base (Irgasan/ Triclosan Ticarcillin Chlorate Broth base ) is recommended for selective enrichment andenumeration of Yersinia enterocolitica .Composition**IngredientsGms / LitreCasein enzymic chloride. (Trichlosan) pH ( at 25 C) **Formula adjusted, standardized to suit performance parametersDirectionsSuspend grams (the equivalent weight of dehydrated medium per litre)in 988 ml distilled water. Heat to boiling to dissolve the medium by autoclaving at 15 lbs pressure(121 C)for 15 to 45-50 add rehydrated contents of 1 vial of Ticarcillin Supplement (FD102) and Potassium Chlorate Supplement (FD103).

Please refer disclaimer Overleaf. ITC Broth Base (TTC Broth Base) M1220 ITC or TTC Broth Base (Irgasan/ Triclosan Ticarcillin Chlorate Broth Base) is recommended for selective enrichment and

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Transcription of ITC Broth Base (TTC Broth Base) - HiMedia Labs

1 Please refer disclaimer Broth base (TTC Broth Base) M1220 ITC or TTC Broth base (Irgasan/ Triclosan Ticarcillin Chlorate Broth base ) is recommended for selective enrichment andenumeration of Yersinia enterocolitica .Composition**IngredientsGms / LitreCasein enzymic chloride. (Trichlosan) pH ( at 25 C) **Formula adjusted, standardized to suit performance parametersDirectionsSuspend grams (the equivalent weight of dehydrated medium per litre)in 988 ml distilled water. Heat to boiling to dissolve the medium by autoclaving at 15 lbs pressure(121 C)for 15 to 45-50 add rehydrated contents of 1 vial of Ticarcillin Supplement (FD102) and Potassium Chlorate Supplement (FD103).

2 Mix well before dispensing in sterile And InterpretationThe genus Yersinia belongs to the family Enterobacteriaceae . They are usually nitrate reductase positiveand show fermentative metabolism. The genus comprises of 11 species, of which Yersinia enterocolitica is mostimportant as a causative agent of human foodborne illness. Variety of enrichment methods has been described for recoveryof Y. enterocolitica from foods. The most efficient procedures for recovering enteropathogenic bacteria from foods haveincorporated at least one and often two enrichment steps before plating onto selective differential agar media.

3 ITC Broth isformulated in accordance with APHA (1) and is recommended by ISO Committee (2) as a selective enrichment medium forY. enterocolitica from foods. ITC Broth was developed by Wauters et al (3) as a new enrichment Broth , derived from modifiedRappaport Broth and based on the selective agents irgasan, ticarcillin and potassium enzymic hydrolysate and yeast extract provide essential growth nutrients. Ticarcillin has inhibitory action on bothgram-positive and gram-negative organisms. Irgasan inhibits gram-positive organisms. Potassium chlorate has enrichment prepare 1: 10 homogenate of food sample by weighing 25 grams of food and adding it to 225 ml of primaryenrichment medium.

4 Prepare homogenate and carefully transfer the homogenate into sterile jar for incubation. After incubation,streak onto agar plates such as MacConkey Agar (M081). After incubation, observe for the colonies of Yersinia , which arepinkish coloured, smooth and have an entire edge. Colonies of Yersinia are larger on agar media when incubated at 25 Cas is more active biochemically at 25 C than at 35-37 ControlAppearanceLight yellow to light blue homogeneous free flowing powderColour and Clarity of prepared mediumPeacock green coloured, clear solution without any precipitateReactionReaction of w/v aqueous solution at 25 C.

5 PH : LaboratoriesTechnical ResponseM1220: Cultural characteristics observed with added Ticarcillin Supplement(FD102) and Potassium Chlorate Supplement(FD103) after an incubation at 25-30 C for 24-48 (CFU)GrowthCultural ResponseEscherichia coli ATCC25922>=10 inhibitedStaphylococcus aureusATCC 25923>=10 inhibitedYersinia enterocoliticaATCC 2772950-100good-luxuriantStorage and Shelf LifeStore below 30 C in tightly closed container and prepared medium at 2-8 C. Use before expiry period on the C. and Splittstoesser D. F., (Eds.), 1992, Compendium of Methods for the Microbiological Examination of Foods,3rd Ed.

6 , APHA, Washington, Organization for Standardization (ISO), 1994, Draft ISO/DIS G., Goossens V., Janssens M. and Vandepitte J., 1988, In. J. Syst. Bacteriol., 38, : 02 / 2015 HiMedia Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-61471919 Email: :User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate.

7 HiMedia Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notbe considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.


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