標準検査法名称 (案) - jslm.org

1 ( )2011/09/15 JCCLS W/GRIA ( )radioimmunoassayIRMA ( )immunoradiometric assay EIA ( heterogeneous enzyme immunoassayELISA (enzyme-linked immunosorbent assay ) EIA ( ) homogeneous enzeme immunoassayEMIT (enzyme multiplied immunoassay technique) CEDIA (cloned enzyme donor immunoassay)LBA ( )liquid-phase binding assayFEIA ( )fluorescence enzyme immunoassayCLEIA ( )chemiluminescence enzyme immunoassayBLEIA ( )bioluminescence enzyme immunoassayFPIA ( )fluorescence polarization immunoassayEV-FIA ( )evanescent wave fluoroimmunoassaySLFIA ( )substrate-labelled fluoroimmunoassayTR-FIA ( )time resolved fluoroimmunoassayCLIA ( )chemiluminescence immunoassayECLIA ( )electrochemiluminescence immunoassayBLIA ( )bioluminescence immunoassayTIA ( )turbidimetric immunoassayLA ( )latex agglutination turbidimetry ( ) KIMS (kinetic interaction of microparticles in a solution)LPIA ( )latex photometric immunoassayPAMIA ( ) particle mediated immunoassayPCIA ( ))

2 Particle counting immunoassay ( )nephelometric immunoassay (Grabar-Williams ) Grabar-Williams immunoelectrophoresis counter immunoelectrophoresis rocket immunoelectrophoresisLaurell crossed immunoelectrophoresis (immunoselection )immunoselection method immunofixation methodSRID ( )single radial immunodiffusionDID ( )double immunodiffusion HA ( )hemagglutinationPHA ( )passive hemagglutinationMPHA ( )mixed passive hemagglutinationRPHA ( )reversed passive hemagglutinationRPLA ( )reversed passive latex agglutinationIAHA ( )immunoadherence hemagglutinationHI ( )hemagglutination inhibition test latex agglutinationPA ( )particle agglutination colloidal gold agglutination ( ) cardiolipin test latex agglutination inhibition test somatic agglutination CF ( )complement fixation test hemolytic reaction testNT ( )viral neutralization test enzyme-neutralization test toxin-neutralization testFA ( )fluorescent antibody techniqueIFA ( ) DFA ( )

3 Enzyme-labelled antibody techniqueOIA ( )optical immunoassay enzyme immunofixation method immobilization test immunochromatography ( ) ( ) ( ) ( ) ( ) ( ) flow-through immunoassay affinity chromatography (TLC)thin layer chromatography (GC)gas chromatography - (GC-MS) gas chromatography-mass spectrometry (HPLC)high performance liquid chromatography - (LC-MS) liquid chromatography-mass spectrometry (GLC)

4 Gas liquid chromatography ultra centrifuge method coil planet centrifuge method paper electrophoresis cellulose acetate membrane electrophoresis agarose gel electrophoresis starch gel electrophoresis polyacrylamide gel electrophoresis polyacrylamide gel disc electrophoresis isoelectric forcusing salting precipitation method potentiometry amperometry coulometry conductimetry arterial blood gas analysis visible absorption spectrometry (UV )ultraviolet absorption spectrometry (IR )infrared absorption spectrometry atomic absorption spectrometry (SBPA)sandwich binding protein assay enzyme cycling method chemical inhibition method immunological inhibition method flame photometry fluoro photometry chemiluminescence photometry ( )refractometry turbidimetry nephelometry (FPA )fluorescence polarization assay densitometry flow cytometry gelation reaction ( )turbidimetric time assay (ICP-MS)

5 Inductivity coupled plasma-mass spectrometry hemacytometer cyan methemoglobin method sodium lauryl sulfate method microhematocrit method automated hematology cell analyzing method whole blood coagulation time thrombin timePT ( )prothrombin timeAPTT ( ) activated partial thromboplastin timeBethesda Bethesda method Russell's viper venom time / ( ) ( ) ( ) platelet neutralization procedure kaolin clotting time antibody neutralization test fibrinolysis time method fibrinolysis area method synthetic (chromogenic) substrate method platelet agglutination test thromboelastography bleeding time platelet retention rate aerobic culture anaerobic culture CO2 culture microaerobic culture ( )mycobacterial culture (Ogawa method) ( )mycobacterial culture (broth culture)

6 Shell vial method disc diffusion method broth microdilution method agar dilution method photometric analysis agar proportion method broth microdilution method broth-based system pyrazinamidase assay tube method agar well method thin layer disc method cup method Southern blotting Northern Blotting Western blotting immunoblotting (LiPA)line probe assay dot blotting slot blottingPRINS PRINS methodFISH fluorescence in situ hybridizationSISH silver in situ hybridizationDual Color in situ Hybridization(DISH) CISH chromogenic in situ hybridization liquid hybridizationSSOP sequence specific oligonucleotide probe technique DNA branched DNA probe method hybrid capture methodHPA hybridization protection assayDKA dual kinetic assayDNA DNA sequencingPCR polymerase chain reaction(PCR)

7 Competitive PCR competitive PCRmultiplex PCR multiplex PCRIM-PCR intercalation monitoring PCR mutation site-specific amplification PCR realtime PCRin situ PCR in situ PCRRT-PCR reverse transcriptase-polymerase chain reactioncompetitive RT-PCR competitive RT-PCRmulticyclic RT-PCR multicyclic RT-PCR PCR RT-PCR ( ) ( ) ( ) RT-PCR realtime RT-PCRin situ RT-PCR in situ RT-PCRLCR ligase chain reactionSDA strand displacement amplificationLAMP loop-mediated isothermal amplificationRT-LAMP reverse transcription loop-mediated isothermal amplificationICAN isothermal and chimeric primer-initiated amplification of nucleic acidsTMA transcription-mediated amplificationNASBA nucleic acid sequence based amplificationTRC transcription-reverse transcription concerted reactionOSNA one-step nucleic acid amplificationSSCP single-strand conformation polymorphismDGGE denaturing gradient gel electrophoresisRFLP restriction fragment length

8 PolymorphismCFLP cleavase fragment length polymorphismSSP polymerase chain reaction-sequence specific primerPHFA preferential homoduplex formation assay invader assayPNA-LNA PCR clamp PNA(peptide nucleic acid)-LNA(locked nucleic acid) PCR clamping neutralization titration redox titration precipitation analysis chelatometric titrationRRA ( )radioreceptor assay CPBA ( )competitive protein binding assayREA ( )radioenzymatic assay3H- 3H-thymidine uptake51Cr 51Cr-release assay (RLA)radioligand assay (RA)daioassaySDI succinic dehydrogenase inhibition testCD-DST collagen gel droplet embedded drug sensitivity testMTT MTT assayHDRA histoculture drug response assay TGP thermoreversible gelation polymer methodHTC human tumor clonogenic assay NDE novel dye exclusion method chromogenic assay chromogenic enzyme assay osmometry optometry viscousmetry bioassay rosette formation test cytotoxicity test calculation