바이오의약품 생산에서 당사슬 ... - ksmcb.or.kr

1 Molecular and Cellular Biology News 1 . , 2013 1,600 , 70% 130 . , (erythropoietin;EPO), , (granulocyte-colony stimulating factor; G-CSF), , . (contract manufacturing organization; CMO).

2 , (post-translational modification; PTM) . , (glycosylation) PTM . , . , CHO (chinese hamster ovary) . , (1, 2). FDA CHO N-glycolylneuraminic acid (NGNA) (1), CHO -gal (2).

3 , CHO and Cellular Biology News 2 7 2011 . , (3). , , . , DNA, RNA .. , (batch).

4 N- O- (N-linked or O-linked glycosylation) . , N- . N- N- (N-glycan) . (dolicholpyrophosphate, PP-Dol) ALG (Asparagine-LinkedGlycosylation) ALG N- (N-acetylglucosamine, GlcNAc), (mannose, Man), (glucose, Glc) , (lipid-linked oligosaccharide,LLO) Glc3 Man9 GlcNAc2 -PP-Dol (4). LLO 8 subunit (oligosaccharyltransferase) co-translational translocation N-x-S/T N- (sequon) (4-5).

5 N- ( -glucosidase I, Gls1p ; -glucosidase II, Glsp II) . lectin chaperone calnexin/calreticulin . Man8 GlcNAc2 (6-7). , . , calnexin/calreticulum cycle . N- (8-9).

6 , , , , ( 1). , , GlcNAc Asn trimannosylcore . trimannosyl core (high-mannose type) . Saccharomyces cerevisiae . (pauci-mannosetype) (9). Molecular and Cellular Biology News 3 (mannosidase IA, IB & IC) Man5 GlcNAc2 N-acetylglucosaminyltransferase (GNT) I GlcNAc II trimannosyl core GlcNAc ( 1).

7 GlcNAc . Asn GlcNAc (1,6)- (fucose) . (1,6)- (1,3)- bi-fucosylated . (1,3)- . GlcNAc (1,3)- , (1,2)- (xylose), GlcNAc (1,3)- (galactose) (1,4)- ( 1) (9). (vacuole) N.

8 (1,3)- , bisecting (1,2)- Lewis a(Lea) . 1. , , N- . N- . Consortium for Functional Glycomics( ) . ( Introduction to Glycobiology Maureen E. Taylor, Kurt Drickamer)Molecular and Cellular Biology News 4 7 2011 (10). trimannosyl core GlcNAc GNT II GlcNAc.

9 GNT IV V ( 1), GNT VI, IX VB 6 . GlcNAc -galactosyltransferase -sialyltransferase GlcNAc .GNT V (1,6)-GlcNAc Gal- (1,4)-GlcNAc- (1,3) polylactosamine , T . , -fucosyltransferase . N- O.

10 , N-x-S/T N- Ser Thr . , Ser Thr Pro (11). , , O- O- (12). O- . GalNAc Ser Thr O- . O- O- , O- , O-GlcNAc O- . hydroxylation hydroxylysine hydroxyproline O.