透析液中エンドトキシンの測定 - LAL試薬

1 E N D O T O X I N. HDF .. ISO JSDT .. 30 . 2.. LAL .. 3.. ISO 2009. 2008 ET ET ET . CFU/mL EU/mL . CFU/mL EU/mL CFU/mL EU/mL . 100. 10 100 Dialysis water 1 50. 100.. 100 Dialysis fluid 50. 1 . Ultrapure Dialysis fluid .. 10-6 . Substitution .. 1 100mL. 1 100mL. 10mL 10 25mL 1000mL.. 10 100mL.. 1 3 1 1 .. 2 1 1 1 1 .. 2 , .. RO RO .. A .. B .. ETRF ETRF ETRF .. ETRF ETRF ETRF .. A B A 40 B 20 . 4. EN D OT O X I N.. LAL .. C .. (1 3)- -D- . 30 . 1EU/L .. LAL .. Endotoxin Factor C Activated Factor C. Factor B Activated Factor B. Proclotting enzyme Clotting enzyme Boc-Leu-Gly-Arg-pNA. Boc-Leu-Gly-Arg-pNA H-Gly Leu-Gly-Arg-Lys Arg-Gly Ser-OH. 1 16 17 18 19 46 47 175. Coagulogen Boc-Leu-Gly-Arg-OH pNA. H-Gly Leu-Gly-Arg-OH Giy Ser-OH. 1 16 17 18 47 175. S-S 2. Coagulin 405nm Gel .. 5.. EG SV-12. ES-24S . 30 . 1EU/L .. EG SV-12. EG SV-12 .. 1 12 . 48 . Code . 900630 EG SV-12 1 980,000. EG SV-12 1 . CD-ROM 2 . 1.

2 1 . 1 . 3 2 1 . 1 .. ES-24S . ES-24S .. Code .. 020170 ES-24S 1 33,000. 4 . LAL C 24 . - 2 . CSE-L . ES-24S .. - . 1 .. Code . 020055 CSE-L 1 10,000. - 6 . O113:H10 3 . 6. EN D OT O X I N.. Code . 900516 200 1 25,000 . 900517 1000 1 25,000 . 900540 200 500 27,000 . 900545 1000 100 16,000 . 800807 100 9,600 ES-24S . 800804 100 1,500 . 800798 ( ) 100 11,000 .. 800826 HD 50 22,000 .. 900675 TM-251 1 30,000 .. ES-24S EG SV-12 .. EG SV-12 . 1. EG SV-12 . 2. EG SV-12 . 3. 15 .. 4. EG SV-12 .. 5. EG SV-12 .. 6.. 7. ES-24S LAL . 8. 1 1 .. 9. LAL 200 L 2 .. 10 11 . 10. LAL St1 200 L 2 . 11. LAL EG SV-12 37 30 .. 12. St2 . 10 11 . 13.. 7.. MP-96. ES-50M . 30 . 1EU/L .. MP-96. MP-96 .. Code . 900625 MP-96 1 1,700,000. MP-96 1 . DOS-V 1 . CD-ROM 1 . 1 . 3 2 1 . 1 .. ES-50M . ES-50M .. - 50 . Code . 020150 ES-50M 1 28,000. 1 . LAL C 1 . - 1 . CSE-L . ES-50M .. - . 1 .. Code . 020055 CSE-L 1 10,000. - 6 . O113:H10 3 . 8.

3 EN D OT O X I N.. Code . 900510 1 33,000 . 900520 50 26,000 . 900570 LP 50 31,000 .. 900516 200 1 25,000 . 900517 1000 1 25,000 . 900540 200 500 27,000 . 900545 1000 100 16,000 . 800804 100 1,500 . 800798 ( ) 100 11,000 .. 800826 HD 50 22,000 .. 900675 TM-251 1 30,000 .. ES-50M MP-96 .. MP-96 . 1. MP-96 . 2. MP-96 . 3. 15 .. 4. MP-96 .. 5. 1 1 .. 6. LP St1 St2 50 .. 7. ES-50M LAL 1 .. 8. ES-50M 50 . 9. MP-96 . 10. Measure . 11. List Result . 9.. T . T .. HD .. 10 .. HD .. HD .. 4 . 7 . HDF .. 10. EN D OT OX I N.. ISO CD 23500 . Step-by-Step . 30 . Pyrosate . Code . 010320 ( ) 10 35,000- .. 900549 50 5,000- .. 1 2008. 41. 3 . , 159-167 2008 . 2 WG: Ver. 2010 . 3 HDF HDF .. 96D HDF . 1 2 pp. 56-73 HDF 1996 . 4 pp. 55-77, . 1995 . 5 Yamamoto, C. and Kim, : Validation of limulus tests for endotoxin evaluation in dialysate. Nephrology, 2, 429-434 1996 . 6 12 HDF 96: 149-158. 1996 . 7 55 68-70. 2003 . 8 59 283-285.

4 2005 . 9 14 1 . , 169-173 2007 . 10 WG , 2010 . 11. 2010 9 1 2T NE.