LC COLUMN SELECTION Column Selection By USP …

1 HICHROM. Chromatography columns and Supplies LC COLUMN . SELECTION . COLUMN SELECTION By USP Specifications Catalogue 9. Hichrom Limited 1 The Markham Centre, Station Road Theale, Reading, Berks, RG7 4PE, UK. Tel: +44 (0)118 930 3660 Fax: +44 (0)118 932 3484. Email: USP Specifications HPLC COLUMN SELECTION BY USP SPECIFICATIONS. LC COLUMN SELECTION USP Specifications The following list of USP ( united states pharmacopoeia ) COLUMN specifications (USP 35) includes a SELECTION of recommended columns within each category. In most cases there are several columns available within a given category, but in a few indicated instances a packing very closely fitting the specification has been included.

2 Please contact us for further advice and assistance on selecting a suitable COLUMN by USP specification . Please also contact us for advice on COLUMN SELECTION by EP (European pharmacopoeia ) specification . The USP monographs allow chromatographers flexibility to make method adjustments within specified limits in order to meet system suitability requirements. Please see page 61 for further details. Octadecylsilane chemically bonded to porous or non-porous silica or ceramic microparticles, to 10 m in diameter, or a monolithic rod L1. Widely available Octadecylsilane chemically bonded to silica gel of a controlled surface porosity that has been bonded to a solid spherical core, 30 to 50 m in diameter L2.

3 Pellicular ODS. Porous silica particles, to 10 m in diameter, or a monolithic silica rod L3. Widely available Silica gel of controlled surface porosity bonded to a solid spherical core, 30 to 50 m in diameter L4. Pellicular silica Alumina of controlled surface porosity bonded to a solid spherical core, 30 to 50 m in diameter L5. Please enquire Strong cation-exchange packing sulphonated fluorocarbon polymer coated on a solid spherical core, 30 to 50 m in diameter L6. Please enquire Octylsilane chemically bonded to totally or superficially porous silica particles, to 10 m in diameter, or a monolithic silica rod L7. Widely available An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, to 10 m in diameter L8.

4 Widely available Irregular or spherical, totally porous silica gel having a chemically bonded, strongly acidic cation-exchange coating, 3 to 10 m in diameter L9. Partisil SCX NUCLEOSIL SA. Nitrile groups chemically bonded to porous silica particles, to 10 m in diameter L10. Widely available Phenyl groups chemically bonded to porous silica particles, to 10 m in diameter L11. Widely available A strong anion-exchange packing made by chemically bonding a quaternary amine to a solid silica spherical core, 30 to 50 m in diameter L12. Please enquire Trimethylsilane chemically bonded to porous silica particles, 3 to 10 m in diameter L13. YMC TMS Hypersil SAS Develosil TMS-UG.

5 Silica gel having a chemicallly bonded, strongly basic quaternary ammonium anion-exchange coating, 5 to 10 m in diameter L14. NUCLEOSIL SB Exsil SAX Partisil SAX. Hexylsilane chemically bonded to totally porous silica particles, 3 to 10 m in diameter L15. Spherisorb C6 Chromegabond C6. Dimethylsilane chemically bonded to porous silica particles, 5 to 10 m in diameter L16. NUCLEOSIL C2 Chromegabond C2. Strong cation-exchange resin consisting of sulphonated cross-linked styrene-divinylbenzene copolymer in the hydrogen form, 6 to 12 m in diameter L17. Hamilton HC-75 H+ PL Hi-Plex H NUCLEOGEL Sugar 810H. For free technical advice and support email Amino and cyano groups chemically bonded to porous silica particles, 3 to 10 m in diameter L18.

6 Partisil PAC Partisphere PAC. Strong cation-exchange resin consisting of sulphonated cross-linked styrene-divinylbenzene copolymer in the calcium form, about 9 m in diameter L19. Hamilton HC-75 Ca2+ NUCLEOGEL Sugar 810 Ca PL Hi-Plex Ca Dihydroxypropane groups chemically bonded to porous silica or hybrid particles, to 10 m in diameter L20. Kromasil Diol YMC Diol NUCLEOSIL Diol A rigid, spherical styrene-divinylbenzene copolymer, 3 to 30 m in diameter L21. Hamilton PRP-1 PLRP-S Shodex RSpak RP18. A cation-exchange resin made of porous polystyrene gel with sulphonic acid groups, about 10 m in size L22. Hamilton PRP-X200 Shodex SUGAR SH1011. An anion-exchange resin made of porous polymethacrylate or polyacrylate gel with quaternary ammonium groups, 7 to 12 m in size L23.

7 TSKgel BioAssist Q COSMOGEL QA Shodex IEC QA-825. 58. USP Specifications HPLC COLUMN SELECTION by USP Specifications (continued). LC COLUMN SELECTION USP Specifications A semi-rigid hydrophilic gel consisting of vinyl polymers with numerous hydroxyl groups in the matrix surface, 32 to 63 m in diameter L24. TOYOPEARL HW-type Packing having the capacity to separate compounds with a molecular weight range from 100-5000 (as determined by polyethylene oxide), applied to neutral, anionic, and cationic water-soluble polymers. A polymethacrylate resin base, cross-linked with polyhydroxylated ether (surface contained some L25. residual carboxyl functional groups) was found suitable TSKgel G2500 PWXL Shodex OHpak SB-802HQ.

8 Butylsilane chemically bonded to totally porous silica particles, to 10 m in diameter L26. Widely available Porous silica particles, 30 to 50 m in diameter L27. YMC Silica LiChroprep Silica Develosil Silica NUCLEODUR Silica A multifunctional support, which consists of a high purity, 100 , spherical silica substrate that has been bonded with anionic exchanger, amine L28 functionality in addition to a conventional reversed-phase C8 functionality Alltech Mixed-Mode C8/Anion Gamma alumina, reversed-phase, low carbon percentage by weight, alumina-based polybutadiene spherical particles, 5 m in diameter with a pore L29 volume of 80 units GammaBond RP1. Ethylsilane chemically bonded to totally porous silica particles, 3 to 10 m in diameter L30.

9 As for L161. A hydroxide-selective, strong anion-exchange resin-quaternary amine bonded on latex particles attached to a core of m macroporous particles L31 having a pore size of 2000 units and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene IonPac AS11-HC. A chiral ligand-exchange resin packing - L-proline copper complex covalently bonded to irregularly shaped silica particles, 5 to 10 m in diameter L32. NUCLEOSIL Chiral-1 CHIRALCEL WH. Packing having the capacity to separate dextrans by molecular size over a range of 4,000 to 500,000 Da. It is spherical, silica-based, and processed L33 to provide pH stability TSKgel G4000 SWXL Shodex PROTEIN KW-800 series Strong cation-exchange resin consisting of sulphonated cross-linked styrene-divinylbenzene copolymer in the lead form, about 7 to 9 m in diameter L34.

10 Hamilton HC-75 Pb2+ Shodex SUGAR SP0810 PL Hi-Plex Pb A zirconium-stabilised spherical silica packing with a hydrophilic (diol-type) molecular monolayer bonded phase having a pore size of 150 . L35. ZORBAX GF-250. A 3,5-dinitrobenzoyl derivative of L-phenylglycine covalently bonded to 5 m aminopropyl silica L36. Hichrom CHIRA-chrom-1. Packing having the capacity to separate proteins by molecular size over a range of 2,000 to 40,000 Da. It is a polymethacrylate gel L37. TSKgel G3000 PWXL Shodex OHpak SB-803HQ. A methacrylate-based size-exclusion packing for water-soluble samples L38. TSKgel G1000-G6000 PWXL Shodex OHpak SB-800HQ Series A hydrophilic polyhydroxymethacrylate gel of totally porous spherical resin L39.