Transcription of MB MISSION BIOTECH 明欣生物科技有限公司
1 MB MISSION BIOTECH 3 10 3 ( ) Website: TEL: 02-26557128 ext. 266 FAX: 02-26557228 E-mail: 1 3 Last Updated: 2012/09 Mass Spectrometry for Protein Identification MALDI-TOF LC/MS/MS (peptide mass fingerprinting, PMF) Microflex MALDI-TOF MS (Bruker Daltonics) QSTAR XL Hybrid LC/MS/MS System (AB SCIEX) LC Packings Nano Flow HPLC System (Thermo Scientific Dionex) MS MS/MS De novo sequencing (Post-translation modification determination) (desalting)
2 (MALDI-TOF MS or ESI-MS) (in-gel or in-solution digestion) (Zip-Tip Clean-up) (MS/MS analysis) MB MISSION BIOTECH 3 10 3 ( ) Website: TEL: 02-26557128 ext. 266 FAX: 02-26557228 E-mail: 2 3 Last Updated: 2012/09 1. 1,000 2. 5,000 ( Zip-Tip ) 3. 1. 2. keratin 3. 4.
3 1mM FA TFA Zip-Tip 5. Coomassie Blue Sypro Ruby In-gel Digestion 6. 7. Acetonitrile Methanol 8. / 30 picomoles 50 picomoles 5 picomoles/ l 10 l 5 picomoles/ l 10 l 100 picomoles 9. 10. ml (eppendorf) MB MISSION BIOTECH 3 10 3 ( ) Website: TEL: 02-26557128 ext.
4 266 FAX: 02-26557228 E-mail: 3 3 Last Updated: 2012/09 1. ( 02-26557128 266) 2. E-mail 3. 4. ( 11503 3 10 3) 5. 6. 7.
