食用油等のグリシドール脂肪酸 ... - mhlw.go.jp

1 - 1 - 22 5 18 DAG 17 7 DAG DAG DAG 8 DAG DAG DAG 2 3 3 - 2 - LC/MS 5ppm DAG 3 166 286 ppm 3 DAG - 3 - 100 mg * 55 C 4 mL * 55 C 3,500 rpm 10 ODS 1 mL 2 mL 4 mL 2 mL 2 mL 200 L 8 mL /2- 1.

2 1 1 mL LC/MS g 80 mL 10 g 4 5 5 - 4 - * 50 80 C GE 40 C 60 C * 105 C GE GE * 5 5 5 g 20 mL * 15 mL 2 10 mL 50 mL 125 mL 30 125 mL 30 2 125 mL 125 mL 30 2 125 mL 125 mL 30 2 - 5 - * 75 C 40 C 60 C * 100 105 C GE LC/MS LC L-column ODS mm 150 mm, 5 m L-column ODS mm 10 mm, 5 m A 17 17 6 v/v/v B 2 min A98%, B2% min A55%, B45% min (A0%, B100%) min A0%, B100% min A98%, B2% min A98%, B2% 1 mL/min 20 L 40 C MS APCI A 500 C 40 AUX 5 340 C SIM m/z 313 m/z 337 m/z 339 Dwell time.

3 - 6 - 2) 3( )7496174 3( )7093166 1-3)-- 2--- 3-( )-( ) 1--- 2--- 3--- 1--- 2--- 3--- 1--- 2--- 3--- 1-( )( )( ) 2-( )( )( ) 3-( )( )( ) 1-( )( )( ) 2-( )( )( ) 3( )( )( )( ) 1-( )( )( ) 2-( )( )( ) 3-( )( )( ) 1( )( )( )(10) 2( ) 3( ) ( )11 1-( )-( ) 2--- 3--- 1-( )-( ) 2--- 3-( )-( ) 1--- 2--- 3--- 1--- 2--- 3--- 1-( )-( ) 2-( )-( ) 3--- 1-( )( )( ) 2-( )( )( ) 3-( )( )( ) 1-( )-( ) 2-( )-( ) 3-( )-( ) 1-( )( )( ) 2-( )( )( ) 3-( )( )( ) 1--- 2--- 3--- 1--- 2--- 3--- 1--- 2--- 3--- 1--- 2--- 3--- A B , ppm1) A B A A A B B B1 5ppm A B 4 A B B A B3 2 B A A BDAG A A- 7 - 4) 4) 1-5)( )-( ) -5)( )-( ) 2-( )-( ) -( )-( ) 3-( )-( ) -( )-( ) 1--( )( ) --( )( ) 2--( )( ) --( )( ) 3--( )( ) --( )( ) 1 -------- 2 -------- 3 -------- 1--( )( )--( )( ) 2--( )( )--( )( ) 3-( )( )( )-( )( )( ) 1 -------- 2 -------- 3 -------- 1-( )-( )-( )-( ) 2-( )-( )-( )-( ) 3-( )-( )-( )-( )

4 2 5ppm 3 , ppm2) A B A4 5 B 1) A B , ppm3)1