Microbiological stabilization of Beer-Mix beverages with ...

1 LANXESS Deutschland GmbH, Paul Marquardt Vienna, Microbiological stabilization of Beer-Mix beverages with dimethyl - dicarbonate first practical experiences 1 Business Unit Material Protection Products DMDC Genie in a bottle Product Launches DMDC Technology Microbiology & Sensory Registration Application 2 Title of presentation Speaker Place, date .. [ OPTIONAL ] Beer-Mix beverages is a very innovative product category 60 84 15 4 4 EuropeNorthAmericaMiddle East& AfricaAsia Pacific Product introduction by Regions 2013* Global product launches by flavour, 2009-13* * Mintel , beer Mixes, Feb 2014 29% 24% 6% 4% 4% 3% 30% LemonLemonadeGrapefruitColaAppleLimeOthe rsProduct launches by packing* 60% 40% 3 Business Unit Material Protection Products DMDC Genie in a bottle Product Launches DMDC Technology Microbiology & Sensory Registration Application 4 Business Unit Material Protection Products DMDC has a unique operating principle lipophilic cell membrane [pH : 2 - 4 (acidic)] [pH.]

2 7 (neutral)] inactivated enzyme with carbo-methoxylated imidazole group penetration effect on 250 mg dimethyl - dicarbonate water 120 mg methanol natural occurring substances 162 mg carbon-dioxide + H3C O O O O CH3 O O 2 + H3C OH 2 CO2 2 H active enzyme with Imidazole group beer Mix Beverage Cell interior NNR2HR1H5 Business Unit Material Protection Products DMDC hydrolyses rapidly, especially in warmer environments 0 % 20 % 40 % 60 % 80 % 100 % 0' 30' 60' 90' 120' 150' 180' 210' 240' Time (Minutes) DMDC 20 C / 68 F 10 C / 50 F 4 C / 39 F Hydrolysis: DMDC + Water = Methanol + CO2 6 Business Unit Material Protection Products DMDC Genie in a bottle Product Launches DMDC Technology Microbiology & Sensory Registration Application 7 Business Unit Material Protection Products DMDC needs to be dosed inline to the finished Beer-Mix beverage with specialized dosing equipment 8 Business Unit Material Protection Products De- centralization of production to sites without tunnel-pasteurizers Flexible use for heat-sensitive packaging Lower energy consumption Reduction of foot print Replacement of tunnel-pasteurizer Improve taste DMDC targets relevant micro- spectrum yeasts Clean label in many countries Remove Persistent preservatives Bottling more sensitive Beer-Mix beverages Switch from sugar-free to full version beer mix beverages Highly flexible application Low investment Utilize existing beer filling

3 Machines Beer-Mix beverages opportunities with DMDC 9 Business Unit Material Protection Products Beer-Mix beverages first experiences with DMDC first Customer Projects: Discussion about Beer-Mix beverages in heat-sensitive packing for export Trials in Wheat Beer-Mix beverage from sugar-free to full version Trials with Radler on existing bottling lines Running business: Customers in Germany with Velcorin in Beer-Mix beverages on existing bottling lines in order to secure Microbiological quality. Velcorin for Beer-Mix beverages 10 Business Unit Material Protection Products DMDC Genie in a bottle Product Launches DMDC Technology Microbiology & Sensory Registration Application 11 Business Unit Material Protection Products Internal Microbiological challenge test microorganisms beer Mix Lager beer + Lemon soda pH = 3,4 Microorganisms Pichia anomala Candida tropicalis Saccharomyces cerevisiae Zygosaccharomyces bailii Bier Mix Wheat beer + Lemon soda pH = 3,4 Matrix Lactobacillus brevis 12 Business Unit Material Protection Products Internal Microbiological challenge test-parameters Parameter beer Mix Lager beer + Lemon soda pH = 3,4 Temperature Germ loading DMDC concentration Value 5-10 C Target.

4 30 CFU/ml and 300 CFU/ml 0 ppm and 250 ppm Replication Duplicates Incubation 18 20 C for 7 days Bier Mix Wheat beer + Lemon soda pH = 3,4 Matrix Nutrient Media MRS Agar & Malt Extract Agar 13 Business Unit Material Protection Products Internal Microbiological challenge test-results MatrixGerm LoadingStart1 WeekStart1 WeekStart1 WeekStart1 WeekMatrix Amount1 mL10 mL1 mL10 mL1 mL10 mL1 mL10 mL54/4920/14640/540+/+20/283/-568/444+/+ 31/3812/10804/640+/+26/25-/-616/520+/+48 /41-/-536/616-/-22/20-/-604/570-/-32/45- /-632/740-/119/31-/-536/636-/- beer Mix - LagerBeer Mix - Wheat0 ppm DMDC250 ppm DMDCI 30 CFU/mlII 300 CFU/mlI 30 CFU/mlII 300 CFU/mlPichia anomala Candida tropicalis MatrixGerm LoadingStart1 WeekStart1 WeekStart1 WeekStart1 WeekMatrix Amount1 mL10 mL1 mL10 mL1 mL10 mL1 mL10 mL1/1-/-84/764/6-/--/-152/1251/3-/3-/-12 3/108-/--/--/-138/12677/452/1-/-68/71-/- -/--/-133/148-/-1/3-/-126/93-/--/--/-127 /165-/1II 300 CFU/ml0 ppm DMDC250 ppm DMDCBeer Mix - LagerBeer Mix - WheatI 30 CFU/mlII 300 CFU/mlI

5 30 CFU/ml14 Business Unit Material Protection Products Saccharomyces cerevisiae Zygosaccharomyces bailii MatrixGerm LoadingStart1 WeekStart1 WeekStart1 WeekStart1 WeekMatrix Amount1 mL10 mL1 mL10 mL1 mL10 mL1 mL10 mL28/26+/+324/252+/+29/2790/98298/314+/+ 34/25+/+312/308+/+24/2724/25294/342+/+24 /22-/-260/256-/-27/27-/-340/266-/-30/30- /-300/308-/-34/20-/-258/234-/-II 300 CFU/ml0 ppm DMDC250 ppm DMDCBeer Mix - LagerBeer Mix - WheatI 30 CFU/mlII 300 CFU/mlI 30 CFU/mlMatrixGerm LoadingStart1 WeekStart1 WeekStart1 WeekStart1 WeekMatrix Amount1 mL10 mL1 mL10 mL1 mL10 mL1 mL10 mL23/271/-274/24016/2330/131/2352/2803/1 30/18-/-260/2735/520/205/9384/28024/2120 /14-/-276/215-/-24/28-/-262/206-/-25/22- /-238/232-/-18/20-/-340/240-/- beer Mix - LagerBeer Mix - Wheat0 ppm DMDC250 ppm DMDCI 30 CFU/mlII 300 CFU/mlI 30 CFU/mlII 300 CFU/mlInternal Microbiological challenge test-results 15 Business Unit Material Protection Products No growth of Lactobacillus brevis with / without Velcorin treatment.

6 Microbe obviously not adapted to the matrix Lactobacillus brevis MatrixGerm LoadingStart1 WeekStart1 WeekStart1 WeekStart1 WeekMatrix Amount1 mL10 mL1 mL10 mL1 mL10 mL1 mL10 mL4/1-/-85/67-/-2/2-/-94/77-/--/--/-69/7 1-/-1/--/-77/74-/-2/--/-65/96-/-2/1-/-90 /51-/-1/7-/-77/64-/-2/--/-84762-/-I 30 CFU/mlII 300 CFU/ml0 ppm DMDC250 ppm DMDCI 30 CFU/mlII 300 CFU/mlBeer Mix - LagerBeer Mix - WheatInternal Microbiological challenge test-results 16 Business Unit Material Protection Products Sensory triangle test no influence on taste Lager type + lemon soda beer Mix Lager type + lemon juice Dealcoholized Lager type + lemon soda Wheat beer + lemon soda Lager type + energy drink Triangle test according MEBAK II No statistical relevant difference No statistical relevant difference No statistical relevant difference No statistical relevant difference No statistical relevant difference Test performed by Forschungszentrum Weihenstephan August 2014: Beverage treated with DMDC against beverage without DMDC 17 Business Unit Material Protection Products Further test at Forschungszentrum Weihenstephan f r Brau- und Lebensmittelqualit t Lager type + lemon soda Microorganisms to be tested Lactobacillus brevis-FZ BLQ 4 Pectinatus frisingensis-FZ BLQ HB S1 Saccharomyces cerevisiae-FZ BLQ H TUM 68 Saccharomyces pastorianus ssp.

7 Carlsbergensis -FZ BLQ H TUM 34/70 beer Mix Saccharomyces cerevisiae var. Diastaticus -FZ BLQ TUM SY 1 Lager type + lemon juice Dealcoholized Lager type + lemon soda Wheat beer + lemon soda Lager type + energy drink Dekkera anomala-FZ BLQ 2-C-1 Wickerhamomyces anomalus FZ BLQ 17-C-3 Kazachstania exigua-FZ BLQ H2-G-7 pH Alcohol [Vol %] BU CO2 [GV-%] [g/100ml] Analytical parameters 18 Business Unit Material Protection Products DMDC Genie in a bottle Product Launches DMDC Technology Microbiology & Sensory Registration Application 19 Business Unit Material Protection Products BMB with Lemon Soda and/ or Juice BMB with Cola, energy drink, etc. Alcohol free BMB Sweetened / Unsweetened BMB Flavoured beer beer with Spirits Malt beer Beer-Mix beverages Categorization Regulatory Categorisation of beer Mix beverages Beverage Category Regulation (EC) No. 1333/2008, Annex II: Category No.

8 : Other alcoholic drinks including mixtures of alcoholic drinks with non-alcoholic drinks and spirits with less than15% of alcohol No registration of DMDC for beer and Malt beverages (Category ) Labelling: Regulation (EC) No. 1169/ 2011, Article 16(4): 4. Without prejudice to other Union provisions requiring a list of ingredients or a mandatory nutrition declaration, the particulars referred to in points (b) and (l) of Article 9(1) shall not be mandatory for beverages containing more than 1,2 % by volume of alcohol. Therefore there is no obligation to label DMDC Outside Focus beer beverages Focused Beer-Mix beverages 20 Business Unit Material Protection Products Not present in final beverage No declaration on ingredients list No allergen or GMO No effect on colour or taste Compatible with all packaging Extensive technical Service Benefits and other applications of DMDC Why Velcorin ?

9 Use of Velcorin in other Beverage categories Carbonated & Still Juice beverages Isotonic Sports beverages Ice Teas Flavoured Waters Wines Ciders 21 Business Unit Material Protection Products THANK YOU FOR YOUR ATTENTION 22 Business Unit Material Protection Products Disclaimer -Presentation is no hand out, for presentation purposes and internal use only! - This information and our technical advice - whether verbal, in writing or by way of trials - are given in good faith but without warranty, and this also applies where proprietary rights of third parties are involved. Our advice does not release you from the obligation to verify the information currently provided - especially that contained in our safety data and technical information sheets - and to test our products as to their suitability for the intended processes and uses. The application, use and processing of our products and the products manufactured by you on the basis of our technical advice are beyond our control and, therefore, entirely your own responsibility.

10 Our products are sold and our advisory service is given in accordance with the current version of our General Conditions of Sale and Delivery. The handling of Velcorin , as a chemical substance, is subject to the German Hazardous Substance Ordinace and the corresponding EC Directive. The following hazard characteristics should be noted: harmful (if swallowed), toxic (by inhalation), corrosive. 23 Business Unit Material Protection Products Due to Velcorin s properties, it can be regarded as a processing aid without requirement for labelling Codex Alimentarius General Standard for Food Additives (Categories and ) Inventory list of substances used as processing aids EU (EC) No 1333/2008 USA Food Additive Provision and Food Contact Notification Halal & Kosher No Labelling Labelling Germany USA Mexico Russia Australia/New Zealand .. Turkey Brazil India China South Africa.