Transcription of miRNA 第一链 cDNA 合成 （加尾法） - sangon.com
1 miRNA cDNA 10 20 2 miRNA P-RT Solution mix 100 l 200 l miRNA P-RT Enzyme mix 20 l 40 l RNase-free water 1 ml 2 ml Universal PCR Primer R(10 M) 100 l 200 l Universal U6 Primer F(10 M) 100 l 200 l 1 1 -20 C miRNA cDNA miRNA cDNA Poly A cDNA 60 miRNA cDNA cDNA 50 Real Time PCR U6 Universal PCR Primer R 1. Poly A cDNA 2. cDNA miRNA 3. U6 4.
2 2 ( ) 1. 2. -20 C 2-8 C 3. RNase 4. 1. Poly(A) 2. 2 miRNA P-RT Solution mix 10 l miRNA P-RT Enzyme mix 2 l Total RNA/micro RNA 2 g/100 ng RNase-free water 20 l 3. 3~5 s 37 C 60 min 85 C 5 min 4 C 4. cDNA 50 #B532451 -20 C 10/20
