National Veterinary Services Laboratories

1 National Veterinary Services Laboratories Reagent ManualReagent NameReagent CodeAmount/ VialTests/ VialBillableReagent Description FY 2022 User FeeAnaplasma marginale enzyme-linked immunosorbent assay (ELISA) proficiency testANP-CHKV ariesVariesA panel of serum samples is used to monitor proficiency of laboratory $ marginale stabilate, Virginia ml1 Virginia strain, stabilate is used to initiate infection in donor animalsYes$ ovis stabilate ovine origin, Idaho ml1 Idaho strain - stabilate is used to initate infection in donor animalsYes$ card test, complement fixation (CF), and competitive enzyme-linked immunoabsorbent assay (cELISA) negative control ml35 Reference control serum for the Anaplasmosis card test, CF, and cELISAYes$ competitive enzyme-linked immunoabsorbent assay (cELISA) high positive control mlReference control serum for the Anaplasmosis cELISAYes$ competitive enzyme-linked immunoabsorbent assay (cELISA) medium positve control ml400 Reference control serum for the Anaplasmosis cELISAYes$ competitive enzyme-linked immunoabsorbent assay (cELISA) negative control mlReference control serum for the Anaplasmosis cELISAYes$ competitive enzyme-linked immunoabsorbent assay (cELISA) positve control mlReference control serum for the Anaplasmosis cELISAYes$ * No charge if used as part of a USDA control program Please note: The NVSL distributes reagents for Veterinary use onlyPrint Date.

2 1/6/2022 Page 1 of 58 Items requested from the National Veterinary Services Laboratories catalog may not be available or may be limited in quantity at any time due to domestic testing or manufacturing NameReagent CodeAmount/ VialTests/ VialBillableReagent Description FY 2022 User FeeAnaplasmosis complement fixation (CF) test positive control ml30 Reference control antiserum for the complement fixation (CF) testYes$ complement fixation (CF) test positive control ml30 Reference control antiserum for the complement fixation (CF) testYes$ complement fixation (CF) test positive control ml30 Reference control antiserum for the complement fixation (CF) testYes$ Susceptibility Proficiency g10 Proficiency Test for Antimicrobial Susceptibility by broth microdilution method Lyophilized pure cultures of various category B bacterial $ adenovirus group I (Quail Bronchitis, chicken embryo lethal orphan) ml1 Cell culture virus propagated in chick embryo kidney cellsYes$ adenovirus group I agar gel immunodiffusion (AGID) ml60 Use to detect antibodies to avian adenovirus group 1 by the AGID test--must be ordered and used in matched sets (same lot numbers) with antiserum 306-ADVYes$ adenovirus group I agar gel immunodiffusion (AGID) ml60 Use as a positive control serum with avian adenovirus group 1 AGID antigen--must be ordered and used in matched sets (same lot numbers) with antigen 303-ADVYes$ adenovirus group III (aka Adenovirus 127, Egg Drop Syndrome 1976, Duck Adenovirus) mlVariesUse in the hemagglutination-inhibition (HI)

3 Test to detect antibodies to adenovirus 127, propagated in duck embryo fibroblast cells (heat-inactivated)Yes$ adenovirus group III (aka Adenovirus 127, Egg Drop Syndrome 1976, Duck Adenovirus) mlVariesUse as a positive control serum in the Adenovirus 127 hemagglutination-inhibition (HI) test, prepared in chickensYes$ encephalomyelitis (AE) ml1 Embryo-derived virus propagated in chick embryosYes$ * No charge if used as part of a USDA control program Please note: The NVSL distributes reagents for Veterinary use onlyPrint Date:1/6/2022 Page 2 of 58 Items requested from the National Veterinary Services Laboratories catalog may not be available or may be limited in quantity at any time due to domestic testing or manufacturing NameReagent CodeAmount/ VialTests/ VialBillableReagent Description FY 2022 User FeeAvian Influenza (AI) H5 Mexican mlPositive Amplification Control (PAC) for Type A influenza H5 rRT-PCR assay .

4 : Must be an APHIS-approved laboratory or in the process of becoming approved. Contact the DVL/AV Section Head for queries or international requests. *$ influenza (AI) H5 transcribed mlVariesPositive control for AIV H5 subtyping RRT-PCR. Transcribed RNA was prepared with PAMP1 plasmid containing the H5 gene of Avian/NY/31588-3/00. : Must be an APHIS-approved laboratory or in the process of becoming approved. Contact the DVL/AV Section Head for queries or international requests. *$ influenza (AI) H7 Eurasian mlVariesAvian Influenza (AI) Eurasian H7 & rRT_PCR Positive Control. : Must be an APHIS-approved laboratory or in the process of becoming approved. Contact the DVL/AV Section Head for queries or international requests. *$ influenza (AI) H7 transcribed mlVariesPositive control for AIV H7 subtyping RRT-PCR. Transcribed RNA was prepared with PAMP1 plasmid containing the H7 gene of CK/NJ/30749-3/00.

5 : Must be an APHIS-approved laboratory or in the process of becoming approved. Contact the DVL/AV Section Head for queries or international requests. *$ influenza (AI) matrix transcribed mlVariesPositive control for AIV matrix RRT-PCR. Transcribed RNA was prepared with PAMP1 plasmid containing the matrix gene of CK/PA/13522-1/98. : Must be an APHIS-approved laboratory or in the process of becoming approved. Contact the DVL/AV Section Head for queries or international requests. *$ * No charge if used as part of a USDA control program Please note: The NVSL distributes reagents for Veterinary use onlyPrint Date:1/6/2022 Page 3 of 58 Items requested from the National Veterinary Services Laboratories catalog may not be available or may be limited in quantity at any time due to domestic testing or manufacturing NameReagent CodeAmount/ VialTests/ VialBillableReagent Description FY 2022 User FeeAvian influenza (AI) proficiency test (AGID or ELISA)AI-CHKN/AN/AAvian influenza (AI) agar gel immunodiffusion (AGID) or enzyme-linked immunosorbent assay (ELISA) proficiency check testYes$ influenza (AI) RRT PCR extraction mlVariesExtraction control for the AI matrix rRT-PCR assay .

6 : Must be an APHIS-approved laboratory or in the process of becoming approved. Contact the DVL/AV Section Head for queries or international requests. *$ influenza immunodiffusion (AIID) negative reference ml12 Use as a negative reference serum in the AIID test for export purposesYes$ influenza immunodiffusion (AIID) negative reference ml12 Use as a negative reference serum in the AIID test for program/surveillance testing *$ influenza immunodiffusion (AIID) strong positive reference ml12 Use as a positive reference serum in the AIID test for program/surveillance testing *$ influenza immunodiffusion (AIID) strong positive reference ml12 Use as a positive reference serum in the AIID test for export purposesYes$ influenza immunodiffusion (AIID) weak positive reference ml12 Use as a weak positive reference serum in the AIID test for export purposesYes$ influenza immunodiffusion (AIID)

7 Weak positive reference ml12 Use as a weak positive reference serum in the AIID test for program/surveillance testing *$ influenza rRT-PCR extraction control - Qiagen ml10 Avian influenza rRT-PCR extraction control for use in the Qiagen extraction. : Must be an APHIS-approved laboratory or in the process of becoming approved. Contact the DVL/AV Section Head for queries or international requests. *$ influenza virus immunodiffusion (AIID) ml120 Use to detect group-specific antibodies to type A influenza viruses--must be ordered and used in matched sets (same lot numbers) with AIID antiserum 305 *$ * No charge if used as part of a USDA control program Please note: The NVSL distributes reagents for Veterinary use onlyPrint Date:1/6/2022 Page 4 of 58 Items requested from the National Veterinary Services Laboratories catalog may not be available or may be limited in quantity at any time due to domestic testing or manufacturing NameReagent CodeAmount/ VialTests/ VialBillableReagent Description FY 2022 User FeeAvian influenza virus immunodiffusion (AIID) antigen (export version) ml120 Use to detect group-specific antibodies to type A influenza viruses for export purposes--must be ordered and used in matched sets (same lot numbers) with AIID antiserum 305-EXPYes$ influenza virus immunodiffusion (AIID) ml120 Use as a positive control serum with AIID antigen--must be ordered and used in matched sets (same lot numbers) with AIID antigen 300 *$ influenza virus immunodiffusion (AIID) antiserum (export version)

8 Ml120 Use as a positive control serum with AIID antigen for export purposes--must be ordered and used in matched sets (same lot numbers) with AIID antigen 300-EXPYes$ paramyxovirus serotype 10 mlAPMV-10, Penguin/Falkland Island/08-000600/2008, beta-propiolactone inactivatedYes$ paramyxovirus serotype 10 mlAPMV-10 Penguin/Falkland Island/08-000600/2008, antiserumYes$ paramyxovirus serotype-2 (APMV-2) ml1 Virus (Chicken/California/Yucaipa/1956 strain) propagated in chicken embryosYes$ paramyxovirus serotype-3 (APMV-3) ml1 Virus (Turkey/Wisconsin/1968 strain) propagated in chick embryosYes$ paramyxovirus type 1 rRT-PCR extraction mlVariesExtraction control for the APMV-1 matrix rRT-PCR assay . : Must be an APHIS-approved laboratory or in the process of becoming approved. Contact the DVL/AV Section Head for queries or international requests. *$ paramyxovirus type 1 rRT-PCR extraction control - Qiagen ml10 Avian paramyxovirus type-1 rRT-PCR extraction control for use in the Qiagen extraction.

9 : Must be an APHIS-approved laboratory or in the process of becoming approved. Contact the DVL/AV Section Head for queries or international requests. *$ * No charge if used as part of a USDA control program Please note: The NVSL distributes reagents for Veterinary use onlyPrint Date:1/6/2022 Page 5 of 58 Items requested from the National Veterinary Services Laboratories catalog may not be available or may be limited in quantity at any time due to domestic testing or manufacturing NameReagent CodeAmount/ VialTests/ VialBillableReagent Description FY 2022 User FeeAvian paramyxovirus type 2 mlVariesUse in the hemagglutination and hemagglutination-inhibition (HI) test. Beta-propiolactone inactivated (P/Ck/CA/Yucaipa/56 strain)Yes$ paramyxovirus type 2 mlVariesAntiserum prepared in chickens against P/Ck/CA/Yucaipa/56 strainYes$ paramyxovirus type 3 mlVariesUse in the hemagglutination and hemagglutination-inhibition (HI) test.

10 Beta-propiolactone inactivated (P/Turkey/Wisconsin/68 strain)Yes$ paramyxovirus type 3 mlVariesAntiserum prepared in chickens against P/Turkey/Wisconsin/68 strainYes$ paramyxovirus type 4 mlVariesse in the hemagglutination and hemagglutination-inhibition (HI) test. Beta-propiolactone inactivated ( strain)Yes$ paramyxovirus type 4 mlN/AAvian paramyxovirus type 4 antiserumYes$ paramyxovirus type 6 mlVariesUse in the hemagglutination and hemagglutination-inhibition (HI) test. Beta-propiolactone inactivated ( strain)Yes$ paramyxovirus type 6 mlVariesAntiserum prepared in chickens against strainYes$ paramyxovirus type 7 mlVariesUse in the hemagglutination and hemagglutination-inhibition (HI) test. Beta-propiolactone inactivated (Dove/TN/4/75 strain)Yes$ paramyxovirus type 7 mlVariesAntiserum prepared in chickens against Dove/TN/4/75 strainYes$ paramyxovirus type 8 mlN/AAPMV-8, Goose/DE/1053/76, beta-propiolactone inactivatedYes$ paramyxovirus type 8 mlN/AAntiserum, APMV-8, Goose/DE/1053/76 Yes$ * No charge if used as part of a USDA control program Please note: The NVSL distributes reagents for Veterinary use onlyPrint Date.