Neural Cell Markers

1 Neural Cell MarkersNeural Cell MarkersNeurons and glia in Neural tissue or cultures are commonly visualized and identified by immunodetection of cell-specific antigenic Markers , including transcription factors, enzymes, cytoskeletal proteins, cell surface proteins, and secreted factors. The Bio-Techne brands R&D Systems and Novus Biologicals together offer an unparalleled selection of antibodies directed against these intracellular and cell surface proteins that can be used for the identification and characterization of different Neural cell types. R&D Systems also offers a variety of different immunnoassays, including the gold standard Quantikine ELISA Kits, for detecting secreted molecules.

2 The Bio-Techne brand Tocris offers a novel and exclusive collection of tools, including bioactive small molecules, caged compounds, and fluorescent probes, for the functional identification of Neural of ContentsNeuronal Markers ..1 8 General Markers ..1 Dendritic Markers ..2 Axonal Markers ..2 Presynaptic Markers ..3 Active Zone Markers ..4 Postsynaptic Markers ..4 Growth Cone Markers ..5 Cholinergic Neuron Markers .. 6 Dopaminergic Neuron Markers ..6 GABA ergic Neuron Markers ..7 Glutamatergic Neuron Markers ..7 Glycinergic Neuron Markers ..8 Serotonergic Neuron Markers ..8 Microglia Markers .

3 9 12 Steady-State Microglia Markers ..9 M1 Microglia Markers ..10 11 M2 Microglia Markers ..12 Astrocyte Markers ..13 General Markers ..13 Oligodendrocyte Markers ..14 15 Oligodendrocyte Precursor Cell Markers ..14 Immature Oligodendrocyte Markers ..14 Mature, Non-Myelinating Oligodendrocyte Markers ..15 Mature, Myelinating Oligodendrocyte Markers ..15 Additional Tools for Visualizing and Identifying Neural Cells ..16 Conjugated Primary Antibodies ..16 R&D Systems VisUCyte HRP Polymer ..16 R&D Systems Multiplex Assays ..16 Tocris Products to Investigate Neural Function ..17 Small Molecules.

4 17 Caged Compounds ..17 Photoswitchable Ligands ..17 Fluorescent Probes ..17 Learn more | MarkersGeneral MarkersNuclearNeuroD1 RBFOX3/NeuNCytoplasmicCalbindin DDoublecortinEnolase 2/Neuron- Specific EnolaseMAP2NF-HNF-LNF-MPSD-93 PSD-95 Taub-III TubulinUCH-L1 R&D Systems and Novus Biologicals AntibodiesMarkerCatalog #SpeciesClonalityApplications Calbindin DAF3320 HPolyWB, IHC Calbindin D-28 KNBP2-50048H M R +MonoWB, ICC DoublecortinNBP1-92684H M R +MonoWB, ICC Enolase 2/Neuron- Specific EnolaseMAB5169 HMonoICC, IHC, IP AF5169H MPolyWB, IHC, SW MAP2 NBP1-92711H M R +MonoWB, ICC MAB8304 HMonoICC, IHC NeuroD1AF2746H MPolyWB, ICC NF-HNB300-135H M R +PolyWB, ICC.

5 IHC, ELISA AF3108 HPolyWB, IHC NF-LNB300-131H M R +PolyWB, ICC, IHC MAB2216 HMonoWB, IHC NF-MNB300-133H M R +PolyWB, ICC, IHC AF3029 HPolyWB, IHC PSD-93NB300-546H M RPolyWB, ICC, IHC PSD-95NB300-556H M R +MonoWB, ICC, IHC, B/N, ChIP, FC, IP RBFOX3/NeuNNBP1-77686H M R +PolyWB, ICC, IHC TauMAB3494 MsMonoWB, IHC AF3494 MsPolyWB, IHC -III TubulinNB100-1612H M RPolyWB, ICC, IHC -III Tubulin (Clone TuJ-1), Neuron-SpecificMAB1195 All SpeciesMonoWB, ICC, SW UCH-L1 , IHC, IP, SWSpecies Key: H Human M Mouse R Rat Ms Multispecies + Additional Species AvailableApplications Key: B/N Blocking/Neutralization ChIP Chromatin Immunoprecipitation CyTOF CyTOF-Ready DB Dot Blot ELISA (ELISA Capture and/or Detection) EM Electron Microscopy FA Functional Assay FC Flow Cytometry GS Gel Shift ICC Immunocytochemistry IHC Immunohistochemistry IP Immunoprecipitation IV In Vitro PEP-ELISA Peptide-ELISA RI Radioimmunoassay SW Simple Western WB Western blot Indicates an R&D Systems antibody Indicates a Novus Biologicals antibodyNeuN in Mouse Brain.

6 NeuN, also called RBFOX3, was detected in immersion-fixed paraffin-embedded sections of mouse brain using a Rabbit anti -Human/Mouse/Rat RBFOX3/NeuN Antigen Affinity-Purified Polyclonal Antibody (Novus Biologicals, Catalog # NBP1-77686). The cerebellar tissue was stained using HRP and DAB (brown), and counterstained with hematoxylin (blue).Enolase 2 in Human Brain. Enolase 2, also called Neuron-Specific Enolase, was detected in immersion-fixed paraffin-embedded sections of human brain using a Human/Mouse Enolase 2/Neuron-Specific Enolase Antigen Affinity-Purified Polyclonal Antibody (Catalog # AF5169).

7 Prior to incubation with the primary antibody, the tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). The tissue was stained using the anti -Sheep HRP-DAB Cell & Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. All cited reagents are from R&D Systems. NF-H in Human Brain. Neurofilament (NF)-H was detected in immersion-fixed paraffin-embedded sections of human brain using a Goat anti -Human NF-H Antigen Affinity-Purified Polyclonal Antibody (Catalog # AF3108).

8 The tissue was stained with the anti -Goat HRP-DAB Cell & Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue). All cited reagents are from R&D Systems2 Learn more | Drebrin 1 MAP2 SAP102 CytoplasmicKinesin 5A Kinesin 5B L1 CAMNF-HNF-LPeripherinTaub-III TubulinCell SurfaceL1 CAMD endritic MarkersAxonal MarkersSelect R&D Systems and Novus Biologicals AntibodiesMarkerCatalog #SpeciesClonalityApplications , IHC Drebrin 1NB100-1951H M R +MonoWB, ICC, IHC, IP AF7739 HPolyWB, ICC, SW MAP2 NBP1-92711H M R +MonoWB, ICC MAB8304 HMonoICC, IHC SAP102 NBP1-87691H M RPolyWB, IHCS elect R&D Systems and Novus Biologicals AntibodiesMarkerCatalog #SpeciesClonalityApplications Kinesin 5 ANBP1-76816H M RPolyWB.

9 ICC, IHC, ELISA Kinesin 5 BNBP2-58451H M RPolyICC, IHC L1 CAMNB100-2682H MMonoWB, ICC, IHC, CyTOF, ELISA, FC, IP AF277 HPolyWB, IHC NF-HNB300-135H M R +PolyWB, ICC, IHC, ELISA AF3108 HPolyWB, IHC NF-LNB300-131H M R +PolyWB, ICC, IHC MAB2216 HMonoWB, IHC PeripherinNB300-137H M R +PolyWB, ICC, IHC, ELISA TauMAB3494 MsMonoWB, IHC AF3494 MsPolyWB, IHC b-III TubulinNB100-1612H M RPolyWB, ICC, IHC b-III Tubulin (Clone TuJ-1), Neuron-SpecificMAB1195 All SpeciesMonoWB, ICC, SWMAP2 in Human Embryonic Stem Cells. Microtubule-Associated Protein 2 (MAP2) was detected in immersion-fixed human embryonic stem cells/neurospheres using a Mouse anti -Human MAP2 Monoclonal Antibody (Catalog # MAB8304).

10 The cells were stained using the NorthernLights 557-Conjugated Donkey anti -Mouse IgG Secondary Antibody (Catalog # NL007; red) and counterstained with DAPI (blue). All cited reagents are from R&D Tubulin in Neonatal Mouse Neurons. bIII Tubulin was detected in fixed dissociated cell cultures of neonatal mouse brain using a Chicken anti -Human/Mouse/Rat bIII Tubulin Antigen Affinity-Purified Polyclonal Antibody (Novus Biologicals, Catalog # NB100-1612; green). The cells were also stained for GFAP (red) and counterstained with DAPI (blue).bIII Tubulin in Differentiated Human Neural Progenitor Cells.