サルモネラ属菌標準試験法 NIHSJ-01-ST4 (090218) …

1 0 NIHSJ-01-ST4 (090218) 090623 (091014 37 C ) 1 ISO ISO ISO 37 C 35 C ISO 35 C 2 1. 25 g BPW 225 ml RV TT TSI LIM O O 2. 3 pH (37 1 C C ) 3.

2 (BPW) 121 C 15 Rappaport-Vassiliadis (RV) 10ml 115 C 15 4 Tetrathionate TT 40 C 20ml lL 10 ml TT MLCB DHL XLD 1 BGS( ) CHS( ) ES II ES II , SM2 TSI (Triple Sugar Iron) 121 C 15 LIM (Lysine Indole Motility) 121 C 15 5 O O O1 O 121 C 15 VP 121 C 15 VP ONPG 4. 1) BPW 37 C 25g BPW 225 1 37 1 C 22 2 6 2) RV TT 42 C BPW ml RV 10 ml BPW 1 ml TT 10 ml RV TT 42 C 22 2 3) RV TT 1 MLCB DHL XLD 7 BGS CHS ESII (ES II) SM2 (chromID Salmonella Agar) 37 1 C 22 2 BGS CHS ESII SM2 4)

3 3 TSI LIM TSI LIM 8 37 1 C 22 2 TSI LIM 5) O O O O 6) 9 5) O O TSI O O1 O O O 6) 10 37 1 C 22 2 VP:VP 37 1 C 22 2 VP A, B 1 ONPG ONPG 1ml 37 1 C 18-24 1-2 VP ONPG 7) 25 25 11 5.

4 25g BPW, 225 ml RV 10 ml TT 10 ml 37 C, 22 2 0. ml ml 42 C, 22 2 H2S MLCB DHL XLD H2S MLCB DHL XLD H2S BGS CHS ESII SM2 H2S BGS CHS ESII SM2 TSI LIM 37 C, 22 2 37 C, 22 2 O 12 6. (Buffered peptone water: BPW) 1,000 ml g g g (12 ) g 1,000 ml 121 C, 15 , pH (Rappaport-Vassiliadis: RV) 1,000 ml g g (KH2PO4) g (MgCl2 6H2O) g g 1,000 ml 13 1.

5 115 C, 15 , (Tetrathionate USA: TT 1,000 ml g g g g g 1,000 ml pH 4 C 45 C 1,000 ml 20ml 10ml g g 14 ml MLCB 1,000 ml g g g g g L- g g g g g g 1,000 ml pH 20ml 15 DHL 1,000 ml g g g g g g g g g g 1.)

6 000 ml pH 20ml XLD 1,000 ml g L- g 16 g g g g g g g g g 1,000 ml pH 20ml BGS BGA 1,000 ml g g 17 g g g g g g 1,000 ml pH BGA 121 C, 15 70 C 60 C 60 C 20ml 2ml 1 g CHS 1,000 ml 18 g g g g g g 1,000 ml pH 20ml ESII (ES II)

7 1,000 ml g g g g g g g 19 g g g g pH 121 C, 15 20ml SM2 (chromID Salmonella Agar) 1,000 ml g g g g g g g g 1,000 ml 20 pH TSI LIM