Transcription of 腸内細菌科菌群の検出試験法 NIHSJ-15-ST1 (増菌培 …
1 1 NIHSJ-15-ST1 ( ) (Enterobacteriaceae) ( ) 25 g 37 C 1 C 18 2 1 ml EE 10 ml 37 C 1 C 24 2 VRBG 37 C 1 C 24 2 1) (Buffered Peptone Water) g NaCl g Na2 HPO4 12H2O g KH2PO4 g 1,000 ml pH 121 C 15 EE (Buffered Brilliant Green bile glucose Broth g g Na2 HPO4 g KH2PO4 g g g 1.
2 000 ml pH 30 2 VRBG ( violet Red bile glucose agar g g g g NaCl g g g 9 g 18 g 1,000 ml pH (Nutrient agar ) g g NaCl g 9 g 18 g 1,000 ml pH 121 C 15 ( glucose agar ) g g g NaCl g g 9 g 18 g 1,000 ml pH 121 C 15 2) N,N,N ,N - -p - g 100 ml 1) 170 C 180 C( ) 2) 121 C( ) 3) 37 C 1 C 4) 44 C 47 C 5) 16 mm 160 mm 20 mm 200 mm 6) 90 mm 100 mm 7) ( 3 mm) / / 8) 1 ml ( ml ) 9)
3 PH 25 C pH 3 10) ( ) (8,000 r/min 45,000 r/min ) 11) g ( ) 1) ( ) 25 g 225 ml 1 2 37 C 18 2 2) 1 ml EE 10 ml 37 C 24 2 3) EE 1 VRBG 37 C 24 2 4) ( ) 37 C 24 2 5) 10 / 37 C 24 2 4 6) /25 g /25 g 1) ISO 21528-1:2004, Microbiology of food and animal feeding stuffs Horizontal methods for the detection and enumeration of Enterobacteriaceae Part1: Detection and enumeration by MPN technique with pre-enrichment 2) ISO 6887-1:1999, Microbiology of food and animal feeding stuffs Preparation of test samples, initial suspension and decimal dilutions for microbiological examination Part1.
4 General rules for the preparation of the initial suspension and decimal dilutions 3) ISO 7218:1996, Microbiology of food and animal feeding stuffs General rules for microbiological examinations, and Amendment1:2001 ( ) 37 C 24 2 1ml EE 10mlVRBG ( )37 C 24 2 1 2 37 C 18 2 25g BPW 225ml37 C 24 2 ( ) 37 C 24 2 /25g
