百日咳の検査診断 - niid.go.jp

1 2019 10 10 Bordetella pertussis Bordetella holmesii DPT-IPV 4 12 16~21 2018 1 30 4 25 LAMP PCR IS481 PT IgG * IgA, IgM *PT.

2 (Pertussis toxin) CDC 1357911 PT IgG IgA, IgM 4 5 2 2 3 1 2 4 C -20 C 1 Bordet- gengou BG 20-40 g/ml CEX CFDN CFDN 4 g/ml CFDN8 g/ml VCM CSM 20 g/ml CEX CA 40 g/ml CEX CFDN CSM 1 36~37 C 4~ 2 7 5 1mm 1 I MALDI-TOF MS MALDI Biotyper LAMP 4 Plex

3 PCR 16S rDNA sequencing2 LAMP (66oC, 40 ) Loopamp D , Cat#LMP54248 2~8 C 12 (ptxP) DNA ( copy/test) , 1 2 PCR Applied Biosystems 7500 Fast PCR 20 l (2xPremix Ex Taq, 10 l; 50xROX dye, l; DNA template, 2 l; Primer mix, 2 l) 95 C 10s, 40 cycles (95 C 3s, 60 C 30s)Kamachi K, New Microbes New Infect, 8:70-74, 2015IS481*(FAM)IS1001(NED)recA(VIC)atpD( Cy5) +--- -+--B. holmesii+-+- ---+ 4 Plex PCR B. holmesii IS481 3) PT IgG EIA 48 PT 20 FHA 20 10 l 2 ml PT IgG FHA IgG WHO 1 3)

4 PT IgG 100 EU/mL <10 EU/mL 10 EU/mL 10 <100 EU/mL 2 10 EU/ml 10 EU/ml 3 IgA, IgM /IgA /IgM /IgM /IgA 2016-10-21 IgA PT FHA IgA , IgM IgM LAMP PT IgG NTU IgA, IgM 7 LAMP PCR * PT IgG IgA, IgM IgA 25-44 IgM 29-56 * Bordetella holmesii, Bordetella bronchiseptica 2018 2018 050010001500200025003000 / / / / 11,947 2018 1 52 n=11,190 <6 5 5 <15 64 30 50 16 5 <15 81 4 2018 1 52 LAMP n=11,190 / 2018 2018 1 EU/ml 5 EU/ml 10 EU/ml 50 EU/ml 100 EU/ml 0204060801000-5M135791113151719212325 (%) PT IgG ( 10 EU/mL)

5 2018 2018 2019 2019 ~ 33 2019 33 2018 2018 300100 -<300<100 286 693 150 606 242 483 , 2018 (IASR Vol. 40 p6-7: 2019 1 ) (IASR Vol. 38 : 2017 2 ) , 2016 (IASR Vol. 38 : 2017 2 ) (IASR Vol. 38 : 2017 2 ) , 2018 (IASR Vol. 40 p12-13: 2019 1 ) TEL:042-848-7102