Transcription of NT-proBNP
1 PATHFAST NT-proBNPPATHFAST NT-proBNP REAGENT FOR PATHFAST 60 DeterminationsB-type natriuretic peptide (BNP) is a small peptide (32 amino acids) secreted by heart myocytes to augment regulation of blood pressure and fluid balance. This peptide is synthesized by ventricular cells and stored as probnp (108 amino acids). probnp is secreted into the bloodstream as the 32 (77 108) amino acids active BNP and the N-terminal fragment of 76 (1 76) amino acids designated NT- Determination of NT-proBNP helps to identify individuals with left ventricular dysfunction. Changes in NT-proBNP concen- tration can be used to evaluate the success of treatment in patients with left ventricular- Stability of NT-proBNP in whole blood samples is 72 hours.
2 Package Content60 determinations 2 calibrators (2x low, 2x high), 2 diluents additionally required: 1 pipette tip per canal (42 pipettes/box)Sample MaterialFor testing 100 l of whole blood or plasma samples are taken with qualified collection tubes containing heparin-Na, heparin-Li, EDTA-Na or Range95 % range (from the to percentile): pg/ml in 102 healthy individuals Specific Performance Data1. Test measuring range: 15 30,000 pg/mlTest PrincipleElecsys is a registered trademark of Diagnostics Method comparison (plasma samples): y = x + ; r = ; n = 795 (y: this method; x: Elecsys probnp ), Further method comparisons on Correlation between whole blood and plasma: y = x + ; r = ; n = 48 (y: whole blood, x: plasma)4.
3 Standardization: The calibrators for PATHFAST NT-proBNP are synthetic NT-proBNP by Roche Diagnostics Detection Limit: 15 pg/mlPATHFAST NT-proBNPM easuring rangePrecisionOverall precisionSampleMean value (pg/ml) (pg/ml)C .V. (%) (pg/ml)C .V. (%)QC-L C- . C- Valli N, Gobinet A, Bordernave L. Review of 10 years of the clinical use of brain natriuretic peptide in cardiology. J Lab Clin Med 1999; 134: 437-4442. Struthers AD. How to use natriuretic peptide levels for diagnosis and prognosis. The European Society of Cardiology. EurHeart J 1999; 20: 1374-13753. Hunt PJ, Richards AM, Nicholls MG, Yandle TG, Doughty RN, Espiner EA.
4 Immunoreactive amino- terminal pro-brain natriuretic peptide ( NT-proBNP ): A new marker for cardiac impairment. Clin Endocrinal 1997; 47: 287-296 4. Talwar S, Squire IB, Davies JE, Barnett DB, Ng LL. Plasma N-terminal pro-brain natriuretic peptide and the ECG in the assessment of left ventricular systolic dysfunction in a high risk population. EurHeart J 1999; 20: 1736-17445. Darbar D, Davidson NC, Gillespire N, Choy A-MJ, Lang CC, Shyr Y et al. Diagnostic value of B-type natriuretic peptide concentrations in patients with acute myo-cardial infarction. A J Cardiol 1996; 78: 284-287 Literatur
