nucleicacid purification INSTAGENE ... - Bio-Rad …

1 Nucleic acid purification TM. INSTAGENE matrix AND KITS. QUALITY- CONTROLLED PCR*-Ready DNA. CHELATING matrix . FOR REPRODUCIBLE in the Palm of Your Hand PCR RESULTS. INSTAGENE matrix INSTAGENE Genomic DNA Kit PCR-READY DNA. Specially formulated, quality-controlled Chelex Yields consistent 50 Kb or larger DNA from FROM TISSUES AND. CELLS IN 30-45 resin for superior PCR results whole blood MINUTES. Reproducible from lot to lot Ideal for long PCR and Southern blotting INEXPENSIVE Offered in ready-to-use, smaller particle slurry Only 30 minutes of hands-on time NO SPIN COLUMNS. that won't clog pipette tips Works with commonly used anticoagulants OR MEMBRANES. REQUIRED Fast and affordable; takes only 45 minutes such as heparin, citrate, EDTA. CONVENIENT. KITS FOR CELLS INSTAGENE Whole Blood Kit INSTAGENE Dry Blood Kit AND TISSUE, Formulated and quality-controlled for optimum Optimum results with dried blood on filter paper WHOLE BLOOD. AND DRY BLOOD results with whole blood Works well with 1/8" or 1/4" paper punches Special buffer speeds up cell lysis step Fast and affordable; takes only 45 minutes Works with commonly used anticoagulants such as heparin, citrate, EDTA.

2 Fast and affordable; takes only 30 minutes INSTAGENE matrix Genomic DNA Kit Whole Blood Kit Dry Blood Kit Treatment of samples with Chelex The molecular diagnostics laboratory In order to increase the efficiency While the whole blood kit contains 100 is the standard procedure for is often interested in preparing DNA of the cell lysis step, Bio-Rad has the INSTAGENE matrix , the INSTAGENE PCR sample preparation from from blood that can be used for designed a lysis buffer to pair up dry blood kit does not. Instead, an bacteria, whole blood, mammalian different diagnostic procedures, with the ready-to-use INSTAGENE extraction and a washing reagent cells and animal tissue. The including short and long PCR and matrix . The INSTAGENE whole blood are supplied. The DNA remains INSTAGENE matrix makes Chelex Southern blotting. These applications kit offers an extremely efficient and bound to the filter paper throughout easier to handle and is QC'd to require a method that consistently fast method for preparing PCR- the procedure and is amplified in ensure reproducibility from lot to lot.

3 Yields pure DNA over 50 Kb in size. ready DNA templates from whole situ. The resulting protocol is fast It's a ready-to-use slurry made with The INSTAGENE genomic DNA kit blood. The protocol for whole blood and simple, as illustrated below. a small particle size for simple meets this criteria quickly and is presented below to illustrate the pipetting, and includes a stirring efficiently, requiring only 30 minutes speed and simplicity of this method. bar for easy resuspension. of hands-on time. Add sample to Mix whole Mix whole Place dried water or PBS.** blood with blood with Lysis blood filter Lysis Buffer. Incubate in 1 ml water. Solution 1. 8 minutes. Incubate Spin, wash Remove 10 minutes, pellet twice with supernatant. twice. Lysis Solution 1. Spin and Wash twice Add Extraction remove Add Lysis with Lysis Reagent and supernatant. Solution 2. Buffer. incubate at Add Protein Remove 65 C for 10. Precipitation supernatant minutes, Add INSTAGENE Solution.

4 Each time. twice. matrix and incubate at 56 C Add INSTAGENE Wash 3 times for 30 minutes. Spin, then matrix and with Wash transfer incubate Reagent. super- at 70 C for Remove natant to 8 minutes. supernatant clean tube. each time. Vortex and incubate at Precipitate Vortex and Wash with 100 C for DNA with incubate ethanol. 8 minutes. ethanol. at 95 C for Remove Wash DNA 4 minutes. super- pellet with natant. 70% ethanol. Spin, use Resuspend DNA Vortex and spin Evaporate supernatant in Rehydration 1 minute at room ethanol and for PCR. PCR Ready Solution. Genomic DNA temperature. PCR Ready use filter for PCR Ready Use supernatant PCR. for PCR. * The Polymerase Chain Reaction (PCR) Ordering Information process is covered by Catalog # Description patents owned by 732-6030 INSTAGENE matrix , 20 ml (enough for 100 samples). Hoffman-LaRoche. 732-6028 INSTAGENE Genomic DNA Kit, one bottle each of Lysis Solution 1, Lysis Solution 2, Protein Precipitation Solution, Rehydration Solution (enough for 100 samples when using 300 l of blood).

5 ** See manual for 732-6211 INSTAGENE Whole Blood Kit, includes one bottle of INSTAGENE matrix and one bottle of INSTAGENE Lysis specific protocols. Buffer (enough for 100 samples). 732-6212 INSTAGENE Dry Blood Kit, includes one bottle of Extraction Reagent and one bottle of Wash Reagent (enough for 125 samples). Bio-Rad Laboratories Life Science Website (800) 4 BIORAD Australia 02 9914 2800 Austria (01)-877 89 01 Belgium 09-385 55 11 Canada (905) 712-2771. Group China 86-10-62051850 Denmark 45 39 17 99 47 Finland 358 (0)9 804 2200 France (01) 43 90 46 90 Germany 089 318 84-0. Hong Kong 852-2789-3300 India (91-11) 461-0103 Israel 03 951 4127 Italy Japan 03-5811-6270 Korea 82-2-3473-4460. The Netherlands 31 318-540666 New Zealand 64-9-4152280 Singapore 65-2729877 Spain (91) 661 70 85 Sweden 46 (0)8 627 50 00. Switzerland 01-809 55 55 United Kingdom 0800-181134. Bulletin 2074 US/EG Rev B 98-019 0398 Sig 120197.