OECD/OCDE TG 487 - nihs.go.jp

1 OECD/OCDE TG 487 2014 9 26 OECD IN VITRO 1. OECD 487 2010 OECD (1) 2. In vitro MNvit MN MNvit (2) (3) in vitro in vitro 13 3.

2 B cytoB cytoB 1 (4) ( 5) 4. MNvit in situ FISH (6) (7) (8) (9) (10) (11) (12) (13) (14) (15) (16) (17) 1 OECD, (2014) OECD OECD TG 487 OECD/OCDE 5. cytoB 1 OECD 473 (18) MNvit 4 FISH 6.

3 MNvit cytoB MNvit (19) (20) (21) (22) (23) (24) (25) (26) (27) (28) (29) (30) (31) (32) (33) (34) (35) (36) Soci t Fran aise de Toxicologie G n tique SFTG (19) (20) (21) (22) (23) International Workshop on Genotoxicity Testing (5) ( 17) EC European Centre for the Validation of Alternative Methods ECVAM ECVAM Scientific Advisory Committee ESAC (37) (38) (39) 7. MNvit (40) p53 DNA 2 OECD, (2014) OECD/OCDE TG 487 8.

4 1 9. In vitro in vivo pH (41) (42) (43) (44) ( 45) 29 10. 1 11. 12. 19 13. 3 OECD, (2014) TG 487 OECD/OCDE 14.

5 (7) ( 20) ( 46) ( 47) CHO V79 CHL/IU L5178Y TK6 (19) (20) (21) (22) (23) ( 26) (27) (28) (29) ( 31) ( 33) (34) (35) (36) 6 HT29 (48) Caco-2 (49) HepaRG (50) ( 51) HepG2 (52) ( 53) A549 (54) CytoB L5178Y (23) 15. 18 35 (55) 48 16.

6 5%CO2 37 C 4 OECD, (2014) OECD/OCDE TG 487 17. 18. cytoB PHA 48 19. 1254 (56) (57) - (58) (59) (60) S9 - (61) 1254 (58) (59) (60) S9 1 2% v/v 10% v/v (62) 20.

7 (63) (64) (65) 5 OECD, (2014) TG 487 OECD/OCDE 21. DMSO 1% v/v CytoB DMSO cytoB 1% v/v 10% v/v 1 cytoB 22.

8 MNvit 1 CytoB (6) ( 66) ( 67) CytoB PHA cytoB 27 cytoB cytoB 23. CytoB CytoB 3 6 g/mL (19) 6 OECD, (2014) OECD/OCDE TG 487 24. 29 30 pH 9 pH pH 25.

9 29 26. cytoB (6) 1 500 CBPI (6) ( 27) ( 68) RI 2 CBPI RI 27. CytoB RPD RICC (17) ( 68) (69) (70) (71) 2 38 39 2 2 3 4 RPD (71) RICC 2 PI 7 OECD, (2014) TG 487 OECD/OCDE RPD RICC 28.

10 3 1 2 2 1 1 3 44, 45 2 (38) 2 3 29 60 3 1 29. 55 5% cytoB RICC RPD cytoB CBPI RI 45 5% (72) 55 5% (71) 30.