Opsys MR Microplate Reader - medteh.info

1 Opsys MR Microplate ReaderUser s GuideIMPORTANTP lease read carefully before using the Opsys MRRevision HistoryThis manual is published by DYNEX TECHNOLOGIES or comments regarding the content of this manual can be directedto the address below or to your DYNEX TECHNOLOGIES Sullyfield CircleChantilly, VA 20151-1683 USAM icrotiter is a registered trademark of DYNEX TECHNOLOGIES INC. 1999 This document is the copyright of DYNEX TECHNOLOGIES andmust not be copied or reproduced in any form without prior reserves the right to make technical improvements to this equipmentand documentation without prior notice as part of a continuous program ofproduct development.

2 This manual supersedes all previous editions. Revision Date: February 1, 1999 Warranty and Special Provisions Limited WarrantyDYNEX TECHNOLOGIES, INC. products are fully guaranteed for one yearagainst defects in parts, materials, and workmanship. Defective parts andmaterials will be replaced or, at the discretion of DYNEX, repaired at nocharge for a period of one year and labor required for such replacement orrepair will be provided at no charge for a period of one year, provided that theproducts are utilized and maintained in accordance with the instructions in theapplicable operating and servicing manual, and provided further that theproducts have not, as determined solely by DYNEX, been subject to misuseor abuse by the Customer or other parties unrelated to DYNEX.

3 DYNEX makes no warranty, expressed or implied, as to the fitness of any product forany particular purposes other than those purposes described in the applicableoperating and servicing manual, nor does DYNEX make any other warranty,whether expressed or implied, including merchantability, other than thoseappearing on the face hereof. Where DYNEX guarantees any product,whether under this Warranty or as a matter of law, and there is a breach ofsuch guarantee, the Customer's only and exclusive remedy shall be thereplacement or repair of defective parts and materials, as described shall be the limit of DYNEXs liability.

4 Furthermore, DYNEX shall not beliable for incidental or consequential damages. Failure of the Customer tonotify DYNEX of a claimed defect by registered mail within thirty days of thediscovery thereof shall constitute a waiver of any claim for breach a product is required by DYNEX to be installed by a DYNEX engineeror technician, the period of this Warranty shall begin on the date of suchinstallation, provided, however, that any use of the product prior to suchinstallation shall, at the sole election of DYNEX., void this Warranty. Wheninstallation by DYNEX personnel is not required, the period of this Warrantyshall begin on the date of shipment from DYNEX.

5 The period of thisWarranty shall begin as described above whether or not the product has beeninstalled or shipped pursuant to a purchase order, and any trail period shall bededucted from the Warranty period that would otherwise apply under asubsequent placed purchase order for that of Liability. Notwithstanding anything to the contrary containedherein, the liability of SELLER (whether by reason of breach of warranty,breach of contract, tort, or otherwise), including without limitation under anyindemnification provision contained herein, shall be limited to replacement ofgoods returned to DYNEX which are shown to DYNEX s reasonablesatisfaction to have been nonconforming or to refund the purchase price, or,if not paid, to a credit amount of the purchase price FOREGOING WARRANTIES ARE EXCLUSIVE AND ARE GIVENAND ACCEPTED IN LIEU OF ANY AND ALL OTHER WARRANTIES,EXPRESS OR IMPLIED, INCLUDING WITHOUT LIMITATION, THEIMPLIED WARRANTY OF MECHANTABILITY AND THE IMPLIEDWARRANTY OF FITNESS FOR A PARTICULAR PURPOSE.

6 NEITHERPARTY SHALL BE LIABLE TO THE OTHER FOR ANY INCIDENTAL,INDIRECT, SPECIAL, OR CONSEQUENTIAL of ContentsTable of ContentsAbout this 1 Chapter 1 3 Effect of 3 Optical 4 Wavelength 5 Single and Dual Wavelength 5 Multiple Wavelength 8 Research 8 Clinical Applications: Infectious 9 Clinical Applications: Automimmune 2 Opsys MR Plate of the a an External on the of Self-Test of ContentsChapter 3 SETUP ENDPOINT up the the the Time and Language, Keypad and Printer Maintenance Communication Reader 4 Assay and Editing a New a an Existing the Test Output the Assay an Existing of ContentsChapter 5 Assay the Assay for Antigen the Assay for Antigen Fitting 6 Running a an of Fit with 7 Recall Plate and 8 9 Routine Maintenance and the and/or Installing a 10 P d137 Table of ContentsDYNEX.

7 137 Czech Republic:..138 USA:..138 United Kingdom:..138 France:..138 Absorbance Module Removal and a Module for A B of C - Values and Fit Linear Curve , Cubic and Quartic Spline Curve Curve Between a Pair of D - Equipment in Transit this ManualThis manual has been written for laboratory technicians andprovides detailed instructions for using the Opsys MR Microplate manual gives you the information needed to: Install the Opsys MR. Set up the Opsys MR to suit your specific applicationrequirements. Understand the Opsys MR menus.

8 Run assays using the Opsys MR. Create or modify assays. Perform required preventive maintenance. Troubleshoot the Opsys MR. Service the Opsys MR. Review safety page is intentionally left blankEffect of PathlengthChapter 1 IntroductionThe Opsys MR is a microprocessor-controlledphotometer. It is designed to measure the optical density(OD) of fluid samples in 96-well microplates in order toquantify the absorbance of various colorimetric of PathlengthLight absorption by a material is determined by the Beer-Lambert law. This states that the:Absorbance of Light is directly proportional tothe product of Pathlength andConcentration.

9 Pathlength is the distance in cm which the light beamtravels through the absorbing material. Absorbance is usually expressed in terms of astandard cell of 1cm pathlength. Concentration is expressed as moles per liter of pathlength through a solution in a Microplate well is lessthan 1 cm. But accurate absorbance values can be obtainedas long as equal volumes of Blanks, Standards and Testsamples are dispensed into wells of equivalent size PathOptical PathFigure 1 illustrates the optical path through the Reader . Atungsten halogen lamp projects a light beam through a heatabsorbing filter and a lens.

10 The beam is focused by the lensand passes through a filter (located on the filter wheel),which allows only light of the desired wavelength range topass. The beam is then separated into 13 channels, one ofwhich is used as a reference channel to monitor the lightoutput of the lamp. The other 12 beams are directedupwards through a row of 12 wells on the Microplate , ontoan array of silicon photodiodes. The silicon photodiodesquantify the intensity of light transmitted through the reactionsolution. Absorbance of the solution is measured in terms ofoptical density (OD) and the assay results are 1.