P515（单页）

1 2 Phanta Max Master Mix P515.. Version Contents 01/ .. 02. 02/ .. 02. 03/ .. 02. 04/ .. 02. 05/ .. 02. 06/ .. 02. 07/ .. 03. 07-1/ PCR .. 03. 07-2/ .. 04. 07-3/ .. 04. 08/ .. 05. 08-1/ .. 05. 08-2/ .. 05. 08-3/ GC .. 08. 08-4/ .. 09. 09/ .. 09. * . 01/ . Phanta Max Super- fidelity DNA Polymerase Phanta Super- fidelity DNA Polymerase . Phanta Max . DNA . Phanta Max 40 kb DNA Phanta Max 20 kb cDNA Phanta Max 10 kb . Taq 1/53 Pfu 1/6 Phanta Max PCR . PCR Phanta Max . 5' 3' 3' 5' . PCR Phanta Max Super- fidelity DNA Polymerase dNTP .. 2 Phanta Max Master Mix . ClonExpress (Vazyme #C112/C113/C115/C601) . 02/ . P515-01 P515-02 P515-03. 2 Phanta Max Master Mix 1 ml 5 1 ml 15 1 ml 03/ . - 30 ~ - 15 0 .. 04/ . DNA cDNA Plasmid DNA PCR . 05/ . DNA / 74 30 min 10 nmol . 1 (U) . 06/ . 1.. 2. dUTP . 3. Phanta Max Super- fidelity DNA Polymerase . TA A DNA . 01/. 02. 4.. 3' G C.

2 3' 8 . 3' . Tm 1 Tm 55 ~ 65 ( Tm Primer Premier 5 ) . Tm . GC 40% - 60% . A G C T GC AT . 5 3' 3 .. NCBI BLAST . 07/ . 07-1/ PCR .. -20 .. ddH2O up to 50 l 2 Phanta Max Master Mix 25 l (10 M) 2 l (10 M) 2 l DNA* x l GC >60% PCR Enhancer(Vazyme #P021). PCR . * 50 l .. DNA 50 - 400 ng DNA 10 pg - 30 ng cDNA 1 - 5 l( PCR 1/10).. a 95 30 sec/3 min 95 15 sec b c 56 ~ 72 . 72 . 15 sec 30 - 60 sec/kb } 25 - 35 cycles 72 5 min a. 95 DNA 30 sec cDNA 3 min . b. Tm Tm 72 ( . PCR) .. c.. 07-2/ . Phanta Max Super- fidelity DNA Polymerase . Touch Down PCR .. 95 3 min 95 15 sec 74 60 sec/kb } 5 cycles 95 15 sec 72 60 sec/kb } 5 cycles 95 15 sec 70 60 sec/kb } 5 cycles 95 15 sec 68 60 sec/kb } 25 cycles 68 5 min . 07-3/ . Phanta Max Super- fidelity DNA Polymerase PCR . PCR . (50 l ). 1 - 5 l . 1 - 2 mm2 .. 1 l . 1 l .. 1 - 2 mm2 . 1 - 5 l . 1 - 5 l .. 100 l Lysis 60 10 min.

3 / . Buffer Proteinase K . 200 g/ml( ) 95 10 min Lysis Buffer 20 mM Tris-HCl, 100 mM EDTA, SDS, pH .. 03/. 04. 08/ . 08-1/ . DNA 2 Phanta Max Master Mix kb kb kb . kb kb kb kb kb kb kb kb kb kb . Tm 60 ( Primer Premier 5 ) .. ddH2O to 50 l 2 Phanta Max Master Mix 25 l 100 ng/ l DNA 1 l (10 M) 2 l (10 M) 2 l .. 95 3 min 95 15 sec .. 60 . 72 . 15 sec 30 sec/kb } 35 cycles 72 5 min . M: DL15,000 DNA Marker 1: kb 2: kb 3: kb 4: kb 5: kb 6: kb 7: kb 8: kb 9: kb 10: kb 11: kb 12: kb M 1 2 3 4 5 6 7 8 9 10 11 12 13 M 13: kb 08-2/ . 1. EDTA ( Vazyme ) 2 Phanta Max Master Mix A B 1,295 bp . 2 Phanta Max Master Mix 3,276 bp 8,513 bp . Tm 60 ( Primer Premier 5 ) .. ddH2O to 50 l 2 Phanta Max Master Mix 25 l * x l (10 M) 2 l (10 M) 2 l * 1 l 2 l 4 l .. 95 3 min 95 15 sec *.. 60 /63 /70 . 72 . 15 sec 30 sec/kb } 35 cycles 72 5 min * kb kb kb 60 63 70 .. 2 Phanta 2 Phanta Max A B Max Master Mix Master Mix M DL15,000 DNA Marker 1 kb(1 l ).

4 2 kb(2 l ). 3 kb(4 l ). 4 kb(2 l ). 5 kb(2 l ). 6 kb(2 l ). * A B .. M 1 2 3 M 1 2 3 M 1 2 3 M 4 5 6 M. 2. ( Vazyme ) DNA( . Vazyme ) 2 Phanta Max Master Mix A . B kb Tm 60 . ( Primer Premier 5 ) .. ddH2O to 50 l 2 Phanta Max Master Mix 25 l * x l (10 M) 2 l (10 M) 2 l * - 3 mm . 05/. 06.. 95 3 min 95 15 sec .. 60 . 72 . 15 sec 30 sec/kb } 35 cycles 72 5 min . 2 Phanta Max A B . Master Mix . M DL 5,000 DNA Marker 1 . 2 . 3 . 4 DNA. * A B .. M 1 2 3 4 M 1 2 3 4 M 1 2 3 4 M. 3. ( Vazyme ) 2 Phanta Max Master Mix A B kb . Tm 60 ( Primer Premier 5 ) .. ddH2O to 50 l 2 Phanta Max Master Mix 25 l 2 l (10 M) 2 l (10 M) 2 l .. 95 3 min 95 15 sec .. 60 . 72 . 15 sec 30 sec/kb } 35 cycles 72 7 min . 2 Phanta Max A B . Master Mix . M DL 5,000 DNA Marker 1 - 6 kb M 1 2 M 3 4 M 5 6 M. 08-3/ GC . 2 Phanta Max Master Mix GC . 654 bp 900 bp 800 bp 1,200 bp 1,400 bp 426 bp GC.

5 68% Tm 60 . ( Primer Premier 5 ) 07-1 .. 95 3 min 95 15 sec 72 45 sec/kb } 35 cycles 72 5 min . M DL 2,000 DNA Marker 1 654 bp GC Content 2 900 bp GC Content 3 800 bp GC Content 4 1,200 bp GC Content 5 1,400 bp GC Content 6 426 bp GC Content M 1 2 3 4 5 6. 07/. 08. 08-4/ . Phanta Max Super- fidelity DNA Polymerase Taq DNA. Polymerase 53 Pfu DNA Polymerase 6 LacI . (Cline, J. et al. Nucleic Acids Research. 24:3546-3551(1996)) .. 80. 70 69 . 60. fidelity Compared to Taq 53 52 51 . 50. 40. 30. 20. 11 12 . 10 8 . 1 . 0. Phanta Phanta Phanta N TA TB Pfu Taq Max EVO. 09/ .. 35 - 40 .. M.. 25 - 30 . Touch down PCR .. 09/. 10. Vazyme Biotech Co., Ltd. Web: Tel: 400-600-9335. Sales: Support: Service.