Transcription of リアルタイム PCR（インターカレーター法）実験ガイド
1 1 1710 PCR TB Green PCR 1 2 3 4 2 17101 TB Green DNA PCR DNA PCR PCR PCR 60 C 95 C PCR Tm Tm PCR GC Primer dimer Raw data 1st Derivative Raw data 1st Derivative Tm Primer dimer 3 17102 20, 200.
2 1000 l ml Vortex ml 8 ml PCR thermal cycler Dice Real Time System TB Green FAM thermal cycler Dice Real Time System Lite TP700 48 thermal cycler Dice Real Time System II TP900 96 thermal cycler Dice Real Time System III with PC TP970 96 PCR thermal cycler Dice Real Time System PCR PCR/RT-PCR 4 1710 thermal cycler Dice Real Time System III with PC TP970 8 8 96 PCR TB Green QuickPrimer (Real Time) PCR QuickPrimer (Real Time) MR101 8 HardFrame Dice 96 well qPCR plate NJ904 Sealing Film for Real Time NJ500 Plate Sealing Pads* 9090 * 96 ml 8-strip tube, individual Flat Caps NJ902 ml 8-strip tube & cap Set NJ903 5 1710 PCR TB GreenTM Premix Ex Taq (Tli RNaseH Plus) RR420S/A/B DNA QuickPrimer (Real Time) Positive Control DNA MR401 3 (1)
3 2 DNA DNA DNA DNA/RNA (2) PCR thermal cycler Dice Real Time thermal cycler Dice Real Time Quick Manual (3) 1 3 1 PCR 12 1 12 2 TB Green Premix Ex Taq 10 l 150 l 5 Primer mix 4 l 60 l 3 l 30 l 1 3 l 3 3 (1) 3 l 6 1710 PCR (4)
4 PCR PCR PCR 4 Ct PCR PCR Ct Ct Ct Ct Tm PCR Tm Tm PCR Tm 7 1710 TB Green Tm 8 1710 Tm Tm
