リアルタイム PCR Probe 法）実験ガイド

1 1703 PCR Probe Probe PCR 1 2 3 4 1703 1 Cycleave PCR RNA DNA PCR RNase H RNA RNA RNase H SNPs 5 5' 3' DNA Taq DNA 5' 3' 1703 2 20, 200.

2 1000 l ml Vortex ml 8 ml PCR thermal cycler dice Real Time System thermal cycler dice Real Time System Lite TP700 48 1703 thermal cycler dice Real Time System II TP900 96 thermal cycler dice Real Time System III with PC TP970 96 PCR thermal cycler dice Real Time System PCR PCR/RT-PCR thermal cycler dice Real Time System III with PC TP970 8 8 8 ml 8-strip tube.

3 Individual Flat Caps NJ902 ml 8-strip tube & cap Set NJ903 1703 96 FrameStar 96 well qPCR plate NJ904 Sealing Film for Real Time NJ500 Plate Sealing Pads* 9090 * 96 1703 PCR TaKaRa qPCR Norovirus (GI/GII) Typing Kit TaKaRa GI/GII ( ) 3 (1) 2 DNA DNA DNA DNA/RNA RNA CycleavePCR O-157 VT gene Screening Kit O-157 VT1/VT2 Typing Kit CycleavePCR SalmonellaDetection Kit CycleavePCR Vibrio tdhgene Detection Kit CycleavePCR Bacillus cereus CRS gene Detection Kit CycleavePCR Listeriamonocytogenes(inlAgene)

4 Detection Kit CycleavePCR Campylobacter jejuni coli Typing Kit CycleavePCR Staphylococcus aureus DnaJgene Detection Kit CycleavePCR (6 ) CycleavePCR Legionella 5S rRNA Detection Kit CycleaveRT-PCR Cryptosporidium(18S rRNA) Detection Kit CycleaveRT-PCR GiardiaDitection(18S rRNA) Detection Kit 1703 (2) PCR thermal cycler dice Real Time thermal cycler dice Real Time Quick Manual (3) 1 3 1 PCR 12 1 12 2 CycleavePCR Master Mix l 150 l 5 Primer/ Probe mix 5 l 60 l dH2O l 30 l 1 dH2O 5 l 3 3 (1) 5 l PCR (4)

5 PCR PCR PCR 1703 Positive Control Plasmid EASY Dilution 1 1 105 copies/ l Positive Control 2 1 104 copies/ l Positive Control 5 l EASY Dilution 45 l 3 1 103 copies/ l 2. 1 104 copies/ l 5 l EASY Dilution 45 l 4 1 102 copies/ l 3. 1 103 copies/ l 5 l EASY Dilution 45 l 5 10 copies/ l 4. 1 102 copies/ l 5 l EASY Dilution 45 l 6 1 copy/ l 5. 10 copies/ l 5 l EASY Dilution 45 l 4 Ct PCR PCR Ct Ct Ct Ct 1 x 105 1 x 104 1 x 103 1 x 102 1 x 101 1 x 100 / l 5 l 5 l 5 l 5 l 5 l 1703 PCR

6 PCR PCR Positive Control Plasmid PCR Positive Control Plasmid CY210 Positive Control Plasmid Positive Control Plasmid 17 1 cfu IC Internal ControlPCR FAM PCR ROX