Transcription of PerkinElmer IVIS 小动物活体成像 解决方案
1 HUMAN HEALTHIN VIVO IMAGINGPE IVIS HUMAN HEALTHENVIREMENTAL HUMAN HEALTHENVIREMENTAL ( ) ( ) : : iRFP( ) HUMAN HEALTHENVIREMENTAL ~ 103p/s ~ 107p/s ~ 7500 ~ 109p/s ~ 109p/s ~ 3 106PC3M-luc/DsRedcells injected Troy, et al. (2004). Molecular Imaging 3:9-23. vs HUMAN HEALTHENVIREMENTAL HUMAN HEALTHENVIREMENTAL Signal / Autofluorescence = 550nm = 610nm = 740nm = 840nmRegular FoodAlfalfa Free Rodent Food = 740nmHUMAN HEALTHENVIREMENTAL - - - - - - - Nluc/Gluc HUMAN HEALTHENVIREMENTAL 200 150 g/ml D-luciferin 2min IVIS 5 HUMAN HEALTHENVIREMENTAL Depilation not required Nude mice SKH1-EDepilation required Normal, haired mouse strains(BALB/c, C57BL/6 etc.)
2 HUMAN HEALTHENVIREMENTAL 3x1013 QDs1x1013 QDs3x1012 QDs A four fold difference in signal QDs were injected subcutaneously in a 20 lvolume. Mice are FVB female on diet :ICGBkgEm:ICG HUMAN HEALTHENVIREMENTAL a b PerkinElmer c 1 mm d abcd HUMAN HEALTHENVIREMENTAL 2. 3. 4. HUMAN HEALTHENVIREMENTAL 14 HUMAN HEALTHENVIREMENTAL Day 7 Day 14 Day 21 Day 0 Day 28 Day 35 Day 4210410510610710810910101011020040060080 006121824303642 Photons/secTumor volumePhotons/secTumor volume mm3 Days post cell implantWith Bioware Ultra one can start collecting data from Day 0, while with caliper measurements one has to wait at least 28 days to see any tumor growth5 cellsBiowareUltra.
3 4T1-luc2 HUMAN HEALTHENVIREMENTAL measurement(tumor still getting bigger)Bio-photonic imaging(tumor cells being killed)Mendel et al2003 HUMAN HEALTHENVIREMENTAL Luciferase BrainBladderKidneyPancreasHUMAN HEALTHENVIREMENTAL DAY 0 DAY 14 DAY 21 DAY 28 DAY 0 DAY 21 DAY 42 DAY 60 DAY 90 Luciferase MDA-MB-231 2*106 VentralDorsalIntracardiacinjection Day 28 Tail vein injection Day 90 VentralDorsalLuciferase HUMAN HEALTHENVIREMENTAL 46 MICEO rthotopicXenograft Model of Liver Tumor (BLI) HUMAN HEALTHENVIREMENTAL Randomized Grouping40 MICECtrlLowMediumHighPositive HUMAN HEALTHENVIREMENTAL Treatment39 MICE CtrlLowMediumHighPositive HUMAN HEALTHENVIREMENTAL PD1 PD1 , Zhen Gu et al.
4 , Nature biomedical engineering, 2018 Nov.(2) 831 840 WT miceCD8 IFN- HEALTHENVIREMENTAL Stephanet al, Nature Biotechnology, 2015ID8-VEGF-luc tumors CBR-lucCAR-T cells T HUMAN HEALTHENVIREMENTAL A Zhanget al, PNAS, 2015,112(31), 9734-9739 4-mo-old miceAPPWTHUMAN HEALTHENVIREMENTAL Prophylactic Atorvastatin TreatmentProSense750 quantifies differences in signal associated with treatment efficacy ApoE HUMAN HEALTHENVIREMENTAL YingGuoet al.,Acta pharmaceuticasinicaB, 2018, 8(2): 200-208 LloviuHUMAN HEALTHENVIREMENTAL DiI RGD HUMAN HEALTHENVIREMENTAL Funct. Mater. 2016 HUMAN HEALTHENVIREMENTAL CT Gas AnesthesiaFiducial PatternIVIS SpectrummicroCT HUMAN HEALTHENVIREMENTAL bioluminescent were co-registered with the thesubject s skeletal anatomy HUMAN HEALTHENVIREMENTAL HEALTHENVIREMENTAL D.
