Transcription of phosphate buffer - University of Nebraska–Lincoln
1 K. M. DeAngelisupdated 1/15/2007phosphate bufferInformation from :Gomori buffers, the most commonly used phosphate buffers, consist of a mixture of monobasic dihydrogenphosphate and dibasic monohydrogen phosphate . By varying the amount of each salt, a range of buffers canbe prepared that buffer well between pH and pH (please see the tables below). Phosphates have avery high buffering capacity and are highly soluble in water. However, they have a number of potentialdisadvantages: * Phosphates inhibit many enzymatic reactions and procedures that are the foundation of molecularcloning, including cleavage of DNA by many restriction enzymes, ligation of DNA, and bacterialtransformation. * Because phosphates precipitate in ethanol, it is not possible to precipitate DNA and RNA from buffersthat contain significant quantities of phosphate ions.
2 * Phosphates sequester divalent cations such as Ca2+ and Mg2+. of 1M K2 HPO4 at mol-1 = of 1M KH2PO4 at mol-1 = of M potassium phosphate buffer at 25 C
