ラットの手術法 - physiology.jp

1 Freshman .. Rattus norvegicus Rat s .. in vivo in vitro .. in vivo .. II .. Freshman .. I .. 26-7G .. Freshman 261.. III stereotaxic apparatus . 1 Pentobarbital sodium . brain map .. 1 . 50 mg/kg . 2 Carbamic acid ethyl ester Urethane . in vivo . 20 25 ear bar . 1 .. 1g/kg . -chloralose 1mm . -chloralose . pH Sodi- . um Borate -chloralose Paxi- nos G and Watson C The Rat Brain in Stero- taxic Coordinates 1 . 100ml 1982 . -chloralose Borate CD-ROM 4 . 100g CD- -chlo- ROM A4 3 . ralose . 1 .. 3 Ketamine hydrochloride AP interaural line . 100mg/kg 1 . 2 Pentobarbitral H.

2 0 reference .. 2 250 . 350 g . 4 Ether 1 incisor bar interaural line mm .. Halothane methoxyflurane . incisor bar .. Isoflu- 2 Strain . rane . Wistar 1998 . Swanson 2 315g S-D .. 262 Vol. 63 No. 10 2001. K nig & Klippel 3 De . Groot 0 . K nig & Klip- . pel 3 150g . incisor bar interaural line . 12mm 16mm .. IV . b .. 1 .. 1 a .. 21G 26G 23G . 1a 30G 1a b .. a 21G 23G .. 1 . a b . c . Freshman 263. 1b . a 1 . b . 26 30G . 1c . coordinates .. L 0mm . superior sagittal . sinus . b . c coordinates Paxi- . nos & Watson A . L H . A . L 0mm H .. 300 l . 10 l . mm 30 1 1 l.

3 Pontamine Skyblue .. H . c . 29G . L 0 4 .. mm .. 0 . 100 m .. d 4 5mm .. Cisterna Magna .. 1a . 264 Vol. 63 No. 10 2001. 2 bipolar electrode . 2 parallel . coaxial . 2b . 23 30G . 5 .. 2 .. 1mm . 1mm .. l . l 1mm . l 1 .. 1 . 2 . 3 .. a 26G . V .. 2c . b 1 cm . monopolar bipolar .. 1 monopolar electrode . c . 2a . A 2 B 1 . b . 1 . Freshman 265. 2 . a b c . 2 .. d 2c .. tip . separation . 2 .. e . SIP 3mm 20 . 2 100Hz .. 5 . 100Hz 50Hz .. 2 .. 3 6 . 266 Vol. 63 No. 10 2001. 1 .. 2mA 10 15 6 8 . tip separation . A 2 . L 0mm H: . 20 . Anteroventral region of the.

4 Third ventricle AV3V . 7 .. VI . 4 3 .. In vivo . a 26G .. Medwire 35 . m 5 8 .. b Polyethylene Gly- col 6,000 PEG 60 70 .. PEG .. PEG . 3 .. Freshman 267. PEG 10 A . c IC 10 200 300 m .. 4 . 2 transpharygeal ventral ap- proach .. a S/N .. 1 .. 2 .. AV3V . 9 .. b . a . 2 . 1 . 90 . IC 4a . dual-type field effect transistor FET b .. IC . 2 c 5 8cm .. c 2 .. 2mm . 7 8mm .. d .. d .. 268 Vol. 63 No. 10 2001. 4 . a b c . AV3V . 2b . 1mm . 3mm .. 1 2mm . 4b in vivo .. e input resistance .. 5 . b . AV3V . 4c . 4c a . 1mm . 1mm 4c . 9 . Freshman 269. AV3V .. c .. Pontamine sky blue 2.

5 20 50 A . 200 500V .. VII .. 1 Paxinos G & Watson C The Rat Brain in Stereotaxic Coordinates. Fourth Edition. Academic Press, 1997.. 2 Swanson LW: Brain Maps : Sturcture of the Rat transcardial perfusion Brain. Second Edition. Elsevier 1998. 3 K nig JF R & and Klippel RA: The Rat Brain. A. Stereotaxic Atlas of the Forebrain and Lower Parts . of the Brain Stem. Williams and Wilkins 1963. 4 Katafuchi T, Oomura Y & Yoshimatsu H: Single neu- ron activity in the rat lateral hypothalamus during 2- deoxy-D-glucose induced and natural feeding behav- ior. Brain Res 359 : 1 9, 1985.

6 5 Katafuchi T & Koizumi K: Fastigial inputs to par- aventricular neurosecretory neurons studied by 10 50ml/min extra-and intracellular recordings in rats. J Physiol (Lond) 421 : 535 551, 1990.. 6 Yoshimatsu H, Oomura Y, Katafuchi T & Niijima A: 50 100ml 10 Effects of hypothalamic stimulation and lesion on ad- 200ml renal nerve activity. Am J Physiol 253 : R418 R424, 1987. 7 Katafuchi T, Oomura Y, Maruyama T & Akaike N: . Hypothalamus and sodium-potassium pump activity in skeletal muscles of DOCA-hypertensive rats. Am J. Physiol. 253 : R396 R401, 1987. 8 Katafuchi T, Ichijo T, Take S & Hori T: Hypothalam.

7 Ic modulation of splenic natural killer cell activity in 10 rats. J Physiol (Lond) 471 : 209 221, 1993. 30 9 Ota K, Katafuchi T, Takaki A & Hori T: AV3V neu- rons that send axons to hypothalamuic nuclei re- spond to the systemic injection of IL-1 . Am J Phys- . iol 272 : R532 R540, 1997.. 270 Vol. 63 No. 10 2001.