Transcription of Pikovskaya’s Broth (medium) - HiMedia Labs
1 Please refer disclaimer s Broth ( medium )M1719 pikovskaya s Broth is recommended for cultivation of phosphate solubilizing **IngredientsGms / LitreYeast **Formula adjusted, standardized to suit performance parametersDirectionsSuspend grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely and sterilize by autoclavingat 15 lbs pressure (121 C) for 15 minutes. Dispense as And InterpretationPikovskaya s Broth is a modification of Pikovskayas agar medium originally modified by Sundara Rao and Sinha (1) forculturing phosphate solubilizing microorganisms.
2 Both inorganic and organic phosphates exists in soil. Many naturallyoccurring soil fungi and bacteria are phosphate solubilizers and they play an important role in maintaining phosphorus balanceof crop plants. This fact is exploited in culturing phosphate solubilzers which are able to solubilize bound phosphates. (2).Phosphate as calcium phosphate is present in the medium . Dextrose acts as energy source. Different salts and yeast extractsupports the growth of organisms. The growth obtained in Pikovsyaayas Broth (M1719) may be detected for phosphatesolubilization by subculturing or spot inoculation on Pikovskayas agar (M520).
3 Phopshate solubilization is indicated asclearance around growth or ControlAppearanceWhite to light yellow homogeneous free flowing powderColour and Clarity of prepared mediumWhitish with flocculant precipitate opaque solution forms in ResponseCultural characteristics observed after an incubation at 35-37 C for 48 hours (by spot inoculation on pikovskaya 's Agar).Cultural ResponseOrganismGrowthPhosphatesolubiliz ation*Cultural Response*Aspergillus brasiliensisATCC 16404luxuriantpositivereaction,clearzone surrounding thecolonyBacillus subtilis ATCC 6633goodModerateclear zonesurrounding thecolonyHiMedia LaboratoriesTechnical DataDisclaimer :User must ensure suitability of the product(s) in their application prior to use.
4 Products conform solely to the information contained inthis and other related HiMedia publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified.
5 Statements contained herein should notbe considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-61471919 Email: Website: notatum ATCC10108luxuriantPositivereaction,clear zonesurrounding thecolonyPseudomonas aeruginosaATCC 27853luxuriantPositivereaction,clearzone surrounding thecolonyStorage and Shelf Life Store below 30 C in tightly closed container and the prepared mediumat 2 - 8 C.
6 Use before expiry date on the Rao and Sinha , 1963, Ind. J., Agric. Sci., 33 Subba Rao, 1977, Soil Microorganisms and Plant Growth , Oxford and IBH Publishing Co., New : 2 / 2015
