PL Hi-Plex Applications - Mono-, Disaccharides

1 PL Hi-Plex ApplicationsCarbohydrate AnalysisCarbohydrates, organic Acids, Alcohols by Ligand Hi-Plex Applications - Mono-, DisaccharidesAnalysis of RaisinsDrying grapes to produce raisins is a simple way ofpreserving a short lived fruit. This chromatogramindicates the sugars found in raisins (sucrose, glucoseand fructose), and shows how the analysis could beused to check for the addition of sugar to inferior raisinsto improve their :PL Hi-Plex Ca, (1170-6810)Eluent:WaterFlow Rate: :85 CDetector:RIKEY1. Sucrose2. Glucose3. Fructose123025min12345 Chewing Gum AnalysisSome chewing gums are sugar free, others containsucrose. In addition, they contain sugar alcohols whichsweeten the product and also act as :PL Hi-Plex Ca, (1170-6810)Eluent:WaterFlow Rate: :85 CDetector:RIKEY1. Sucrose2. Glucose3. Fructose4. Mannitol5. Sorbitol12345025minAnalysis of Fruit JuicePL Hi-Plex Ca columns are ideal for the determination ofthe major sugars in fruit juices.

2 A comparison of orange,apple and tomato juice identifies the varying proportionsof sucrose, glucose and fructose present in each :PL Hi-Plex Ca, (1170-6810)Eluent:WaterFlow :85 CDetector:RIKEY1. Sucrose2. Glucose3. Fructose4. SorbitolTomato Juice Apple Juice Orange Juice 123020min123020min1234025minThe PL Hi-Plex series of 8 m crosslinked materials is recommended for the analysis of mono- anddisaccharides. For monosaccharides, the PL Hi-Plex Pb material is most retentive, and shows improvedresolution for some Disaccharides . The PL Hi-Plex Ca columns are preferred for rapid profiling ofmonosaccharides in the presence of oligosaccharide PL Hi-Plex Ca columns are ideal for the analysis of most sweeteners, monosaccharides (glucose andfructose), disaccharide (sucrose) and sugar alcohols. Rapid profiles of these components in foodproducts can be achieved and compositions quantified for authenticity of Maple SyrupThe main sugar present in this popular American syrup issucrose.

3 Sucrose gives the syrup its sweet taste. PL Hi-Plex Ca gives a good separation of sucrose fromthe more minor components of fructose, glucose andlarger polymeric material, in a relatively short :PL Hi-Plex Ca, (1170-6810)Eluent:WaterFlow :85 CDetector:RIKEY1. Polysaccharides2. Sucrose3. Glucose4. Fructose5. Sugar Alcohol