Potato Dextrose Agar - HiMedia Labs

1 Please refer disclaimer Dextrose AgarMH096 Potato Dextrose agar is recommended for the cultivation of yeasts and moulds from pharmaceutical products in accordance with the microbial limit testing by harmonized methodology of USP/EP/ **IngredientsGms / LitreInfusion from after sterilization ( at 25 C) **Formula adjusted, standardized to suit performance parametersDirectionsSuspend grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize byautoclaving at 15 lbs pressure (121 C) for 15 minutes or as per validated cycle. Mix well before dispensing. In specific work,when pH is required, acidify the medium with sterile 10% tartaric acid. The amount of acid required for 100 ml. of sterile,cooled medium is approximately 1 ml. Do not heat the medium after addition of the And InterpretationYeast and moulds constitute a large and divergent group of microorganisms consisting of several thousands species.

2 Yeastand moulds can cause various degrees of food decomposition. Invasion and growth may occur on virtually any type of foodif environmental conditions are not limiting. Some foodborne yeasts and moulds are undesirable because of potential hazardsto human and animal health (1). Potato Dextrose agar , prepared in accordance with the harmonized methodology of USP/EP/BP/JP (2,3,4,5) is recommendedfor microbial limit tests in pharmaceutical testing. It is also used for stimulating sporulation, for maintaining stock cultures ofcertain dermatophytes and for differentiation of typical varieties of dermatophytes on the basis of pigment production (6). Potato infusion and Dextrose promote luxuriant fungal growth. Adjusting the pH of the medium by tartaric acid to inhibitsthe bacterial growth. Heating the medium after acidification should be avoided as it may hydrolyse the agar , which can renderthe agar unable to solidifyQuality ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with agar gelColour and Clarity of prepared mediumLight amber coloured clear to slightly opalescent gel forms in Petri platespH of w/v aqueous solution at 25 C (after sterilization).

3 Promotion TestGrowth Promotion was carried out in accordance with the harmonized method of USP/EP/BP/JP, and growth was observedat 20-25 C for specified time. Recovery rate is considered as 100% for fungus growth on Sabouraud Dextrose AgarGrowth Promoting PropertiesGrowth of microorganism comparable to that previously obtained with previously tested and approved lot of medium occursat the specified temperature for not more than the shortest period of time specified inoculating <= 100 cfu HiMedia LaboratoriesTechnical DataCultural ResponseMH096: Cultural characteristics observed after incubation at 20-25 C for 2-5 days. Recovery rate is considered as 100% forfungus growth on Sabouraud Dextrose (CFU)GrowthObserved Lotvalue (CFU)RecoveryIncubationtemperatureIncubationperiodTest strain preparation*Aspergillus brasiliensisATCC 1640450 -100luxuriant25 -100>=50 %20 -25 C5 -7 DayAdditional MicrobiologicalTestingCandida albicans ATCC1023150 -100luxuriant35 -100>=70 %20 -25 C2 -3 DaySaccharomyces cerevisiaeATCC 976350 -100luxuriant35 -100>=70 %20 -25 C2 -5 DayRhodotorula mucilaginosaDSM 70403luxuriant20 -25 C3 -5 DayGeotrichum candidum DSM1240good- luxuriant25 -30 C3 -5 DayPenicillium communaeATCC 10248fair -good25 -30 C3 -5 DayTrichophyton ajelloi ATCC28454fair-good25 -30 C3 -7 DayStorage and Shelf LifeStore below 30 C in tightly closed container and the prepared medium between 2 - 8 C.

4 Use before expiry date on the F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,APHA, Washington, United States Pharmacopoeia, 2016, The United States Pharmacopoeial Convention. Rockville, Pharmacopoeia, 2016, The Stationery office British Pharmacopoeia, 2014, European Dept. for the quality of Pharmacopoeia, J., 1985, Media for the Isolation-Cultivation-Identification-Mai ntenance of Medical Bacteria, , Williamsand Wilkins, BaltimoreRevision : 02/ 2016 Key :*- Formerly known as Aspergillus nigerHiMedia Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-61471919 Email: :User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate.

5 HiMedia Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notbe considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any fumigatusATCC 919750 -100luxuriant25 -100>=50 %20 -25 C5 -7 Day