Prevent Defense - Vitalabs

1 PreforPro supports the growth of beneficial bacteria in the gut through a novel prebio c that s not fiber or starch-based, and requires a significantly smaller dosage than typical prebio DefensePreforPro Prebiotics and Probiotics DefinedA Probiotic-Enhancing PrebioticThe Food and Drug Administra on (FDA) defines a prebio c as a non-diges ble food ingredient that beneficially affects the host by selec vely s mula ng the growth and/or ac vity of one or a limited number of bacteria in the colon, and thus improves host health. The FDA defines a probio c as a living microorganism which, when administered in adequate amounts, confer health benefits to the host. These microorganisms are usually bacteria and are some mes referred to as healthy , friendly , good or beneficial cs are microorganisms similar to those that naturally exist in the gut. The idea is that in order to stay healthy, we must maintain a delicate balance of microflora ( a mix of different bacteria) in the gastrointes nal tract.

2 That balance can easily be upset if unwanted bacterial popula ons become predominant in the GI new Prevent Defense is arguably one of the most effec ve Prebio c / Probio c formula ons on the market making use of two primary unique ingredients, PreforPro and bacillus sub lus DE111 . Unlike conven onal prebio cs, such as a type of fiber or starch, PreforPro is a Ly c Bacteriophage cocktail formulated to support healthy levels of good bacteria as opposed to unhealthy strains such as E. coli. DE111 is a clinically researched probio c strain of bacillus sub lus. This spore forming bacteria does not require refrigera on to remain ac ve and is able to ndwithstand heat and other adverse condi ons much more so than nonspore forming strains. In addi on, Prevent Defense contains a 2 spore forming probio c strain, bacillus coagulans, as well as Lactobacillus acidophilus and Bifidobacterium lac hope the following in-depth informa on on PreforPro and DE111 will be of help in reflec ng some of the benefits provided by Prevent Defense : Balancing Bacterial FloraPreforPro In ActionPrebiotics:Benefits and DrawbacksPreforPro : A NewGeneration of PrebioticsA prolifera on of harmful bacteria in the gut can rob your body of the essen al nutrients it needs by consuming those nutrients that your body would normally absorb.

3 Symptoms of unbalanced bacterial flora include : Abdominal pain Indiges on Bloa ng Food allergies Malnutri onIn many cases, good bacteria have a difficult me displacing the unwanted bacteria and require help; this is where prebio cs come into play. Prebio cs are generally fibers or starches ( , oligosaccharides) that have been shown to be beneficial; however these can have some drawbacks , including: Large dosages are required to be effec ve They can cause flatulence They are sensi ve to their specific environment They only work in the colonAs a result of extensive research, the scien sts at Deerland Enzymes have developed a novel prebio c that supports the growth of healthy bacteria in the gut through a mechanism that is not fiber or starch-based. PreforPro addresses the drawbacks of typical prebio cs on the market; benefits include: Efficacious in small doses within hours (not days) Func ons in both the small and large intes nes Does not cause flatulence Not affected by varying gut environments Works with a broad spectrum of probio c speciesPreforPro has been shown under both physiological condi ons in-vitro and in-vivo to preferen ally promote the growth of beneficial Lactobacillus, Bifidobacterium and bacillus sub lis strains when compe ng with undesirable bacterial strains.

4 The effects are achieved at small doses within hours, in both the small and large intes 4. Subjects taking the placeboexperienced a larger increase in cholesterollevels than those taking DE111. Figure 5. Subjects taking the placeboexperienced an increase in glucose levels,while the levels of subjects taking DE111 were 6. Subjects taking the placeboexperienced an increase in triglyceridelevels, while the levels of subjects takingDE111 were maintained. Cholesterol Pre (<200 mg/dL)Cholesterol Post (<200 mg/dL)mg/dLPlaceboGlucose Pre (70-98 mg/dL)Glucose Post (70-98 mg/dL)Placebomg/dLTriglyceride Pre (33-137 mg/dL)Triglyceride Post (33-137 mg/dL)Placebomg/dL - DE111 bacillus subtilis Deerland Enzymes has genome sequenced and clinically tested a highly effec ve strain of bacillus sub lis, a very stable probio c spore that works as a complement to many of the non-spore strains on the market Probiotic PossibilitiesProbiotics for Digestive and Immune HealthThe human body carries nearly 100 trillion bacteria in the 's more than 10 mes the total number of human cells in the en re body.

5 Probio cs are those good bacteria that help keep the intes nes healthy and assist in diges on and nutrient absorp on. Researchers are also finding evidence that certain bacteria in the gut influence the development of aspects of the immune system , . In fact, the gut accounts for 25% of the immune cells in the body which provides 50% of the body's immune cs' main benefit is that they help restore balance in the intes nal microbiota. By enhancing the intes nal flora, these microorganisms may have a larger effect in terms of keeping people in good health. Understanding the type and quan ty of microorganisms in the gut has become a cri cal goal in the pursuit of overall wellness. Consumers today have the ability to influence their gut microbiota like never before from supplements to food, people are seeking sources of good majority of probio cs currently available are bacteria which are non-spore formers, such as Lactobacilli and Bifidobacteria.

6 These probio c strains have been widely studied for their health benefits and are a popular choice for use in dietary supplements or cold-processed foods, such as yogurt. Spore forming bacteria are a diverse group of very hardy bacteria, characterized by their ability to form endospores to protect themselves when condi ons are not favorable. The bacillus sub lis species of microorganism has been known for more than 100 years, having Probiotics Strains: Spore and Non-Spore FormersControls Microbial PopulationsBacillus Subtilis - DE111first been isolated and described in the 1800s. It is considered to be a normal, albeit minor, inhabitant of the gut in animals and humans . bacillus sub lis has the ability to form spores that protect the microbes from harsh condi ons un l they enter an environment ripe for germina on, such as the GI tract. Because of this spore-forming ability, bacillus sub lis offers addi onal benefits as a probio c: Remains viable under a wide temperature range, doesn't require refrigera on Survives passage through the acidic environment of the GI tract Can persist in the GI tract, increase its numbers and then re-sporulate Supports the normal immune reac on of intes nal cells.

7 Crowds out bacterial pathogens and maintains healthy gut flora , . Communicates with intes nal cells to maintain gut barrier func onEach type and strain of probio c, spore and nonspore forming, performs a different role with par cular benefits in terms of diges ve and immune systems' health, as well as where in the GI tract they act. Mul strain probio c supplements provide a broad spectrum of Enzymes has genome sequenced and tested a highly effec ve strain of bacillus sub lis, a probio c spore that works as a complement to many of the non-spore strains on the market today. DE111 has been fully sequenced and uploaded to GenBank, the Na onal Ins tutes of Health gene c sequence 1. Subjects who were administered theplacebo demonstrated a slight decrease inintes nal levels of the probio c bacillus sub lis,while those who were administered DE111 experienced a significant 2. Subjects who were administered theplacebo demonstrated a decrease in intes nallevels of the probio c Bifidobacterium,while those who were administered DE111 experienced a significant 3.

8 Subjects who were administeredthe placebo demonstrated an increase inlevels of the pathogenic bacteria E. coli,while those who were administered DE111 experienced a slight is a classic bacillus sub lis strain that supports the normal prolifera on of beneficial bacteria and crowds out other Gibson, and Roberfroid, , Dietary Modula on of the Human Colonic Microbiota: Introducing theConcept of Prebio cs, Journal of Nutri on, 125: 1401-1412 (1995).2. Fuller, R., Probio cs in Man and Animals, Journal of Applied Bacteriology, 66: 365-378 (1989)3. Food and Agriculture Organiza on and World Health Organiza on, Health and Nutri onal Proper es ofProbio cs in Food including Powder Milk with Live Lac c Acid Bacteria (1-4 October 2001).4. Elphick HL, Elphick DA, Sanders DS. Small bowel bacterial overgrowth. An underrecognized cause ofmalnutri on in older adults. Geriatrics. 2006 Sep;61(9) Handbook of Prebio cs and Probio cs Ingredients, CRC Press, Boca Raton, FL Cummings JH, Macfarlane GT, Englyst HN.

9 Prebio c diges on and fermenta on. Am J Clin Nutr. 2001 Feb;73(2 Suppl) Fiorini G, Cimminiello C, Chianese R. II B. sub lis come s molatore sele vo delle IgA linfocitarie di membrana. ;9:331 Meroni P L, Palmieri R, Barcellini W, De Bartolo G, Zanussi C. Effect of long-term treatment with B. sub lis on the frequency ofurinary tract infec ons in older pa ents. Chemioterapia. 1983;2:142 Novelli A, Ulivelli A, Reali E F, Mannelli F, Trombi-Belcari L, Spezia R, Peri P. bacillus sub lis spores as a natural pro-host oralagent. Preliminary data in children. Chemioterapia. 1984;3:152 Bonomo R, Luzi G, Frielingsdorf A, Aiu F. Ruolo delle IgA secretorie nelle funzioni dell'immunita locale dell'apparatodigerente. Impiego di spore di B. sub lis in alcune forme morbose con deficit di IgA e ipogammaglobulinemia. ChemioterAn microb. 1980;3:237 Ciprandi G, Scordamaglia A, Venu D, Caria M, Canonica G W. In vitro effects of bacillus sub lis on the immune 1986;5:404 Vacca A, Pantaleo G, Ronco M, Dammacco F.

10 Chemoimmunotherapy for mul ple myeloma using an intermi ent combina ondrug schedule (melphalan + prednisone) and alterna ng course of B. sub lis spores. Chemioterapia. 1983;2:300 Mazza P. The use of bacillus sub lis as an an diarrhoeal microorganism. Boll Chim Farm. 1994;133:3 statements have not been evaluated by the FDA. This product is not intended to diagnose, treat, cure, or Prevent any disease. GMP Certified FacilityReferences.