Transcription of 培養細胞への ... - promega.co.jp
1 DNA MultiFectam DNA B MultiFectam DNA MultiFectam MultiFectam 96 - well 2 DNA /DNA DNA DNA A,B,C 3 5-20 l 96well 1well 1 2 104 2 10 105 100 l well DNA MultiFectam MultiFectam 1ml/vial I 90 l + MultiFectam 0 l 90 l II A DNA g/70 l + MultiFectam 20 l 90 l III B DNA g/60 l + MultiFectam 30 l 90 l IV C DNA g/50 l + MultiFectam 40 l 90 l (V* 60 l + MultiFectam 30 l 90 l ) 30 (I-V) OPTI-MEM 30 l 120 l ( * MultiFectam ) 5 well 5/10/20 l 24 Luciferase ( , Cat# E1320) DNA ONE-Glo Luciferase Assay System (Cat.)
2 # E6110) CellTiter-Fluor Cell Viability Assay (Cat.# G6080) 1 overconfluent 95 75-90% DNA DNA 260/280 PureYield 24 48 Tel: 03-3669-7980 / Fax: 03-3669-7982 E-mail: 5 l 10 l 20 l I II III IV V
