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QUANTITATIVE ESTIMATION OF LOSARTAN POTASSIUM IN ...

IJRPC 2011, 1(3) Rajesh et al. ISSN: 2231 2781. INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY. Available online at Research Article QUANTITATIVE ESTIMATION OF LOSARTAN POTASSIUM IN. PHARMACEUTICAL DOSAGE FORMS BY UV. SPECTROPHOTOMETRY. Rao PLKM, Venugopal V, Anil Kumar G, Rajesh B*, Prasad GAL and Ravindergoud D. Department of Pharmaceutical Analysis, SLC's college of Pharmacy, Hyderabad, Andhra Pradesh, India. *Corresponding Author: ABSTRACT. A literal and specific standard second derivative UV- spectrophotometric method was raised for the ESTIMATION for LOSARTAN POTASSIUM in solid pharmaceutical dosage form. The -max of LOSARTAN POTASSIUM was found to be 234nm to both crude and marketed sample and is analyzed using Beer-Lamberts law. Beer's law was obeyed at the concentrations ranging 8-22. g/ml. The second derivative spectrum shows results that were resolved through excipients. The developed methods were absolute, definite, explicit and consistent and found to be a prototype for routine determination for LOSARTAN POTASSIUM .

IJRPC 2011, 1(3) Rajesh et al. ISSN: 2231 2781 295 INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com QUANTITATIVE ESTIMATION OF LOSARTAN POTASSIUM IN

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1 IJRPC 2011, 1(3) Rajesh et al. ISSN: 2231 2781. INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY. Available online at Research Article QUANTITATIVE ESTIMATION OF LOSARTAN POTASSIUM IN. PHARMACEUTICAL DOSAGE FORMS BY UV. SPECTROPHOTOMETRY. Rao PLKM, Venugopal V, Anil Kumar G, Rajesh B*, Prasad GAL and Ravindergoud D. Department of Pharmaceutical Analysis, SLC's college of Pharmacy, Hyderabad, Andhra Pradesh, India. *Corresponding Author: ABSTRACT. A literal and specific standard second derivative UV- spectrophotometric method was raised for the ESTIMATION for LOSARTAN POTASSIUM in solid pharmaceutical dosage form. The -max of LOSARTAN POTASSIUM was found to be 234nm to both crude and marketed sample and is analyzed using Beer-Lamberts law. Beer's law was obeyed at the concentrations ranging 8-22. g/ml. The second derivative spectrum shows results that were resolved through excipients. The developed methods were absolute, definite, explicit and consistent and found to be a prototype for routine determination for LOSARTAN POTASSIUM .

2 The method was validated statistically and by recovery studies .The LOD (limit of detection) and LOQ (limit of quantification) for second derivative spectra were found to be g/ml correlation coefficient value was found to be The purity was found to be 98%.. Keywords: LOSARTAN POTASSIUM , Methanol, UV-visible spectrophotometer INTRODUCTION. LOSARTAN POTASSIUM (2-butyl-4-chloro-1-{[2'- Second Derivative Spectroscopy (1H-tetrazol-5-yl) biphenyl-4-yl] methyl}-1H- The second derivative spectrum characterised imidazol-5-yl) methanol is used in the by two satellite maxima and an inverted band treatment of hypertension. of which the minimum corresponds to the - max of the fundamental band. Experimental Method UV Visible Spectroscopy Insrument Labindia Ltd. UV 3000+, with bandwidth of 1nm, wavelength accuracy of and matched quartz cells are used. UV win software was used. Chemicals Structure of LOSARTAN LOSARTAN POTASSIUM , methanol, distilled water 295. IJRPC 2011, 1(3) Rajesh et al.

3 ISSN: 2231 2781. Preparation of Standard Stock Solution area. The amplitude of the respective A standard stock solution (1000 g/ml) was derivative spectrum is converted in terms of prepared by dissolving accurately 50mg of absorbance. Beer's law was obeyed by the crude LOSARTAN POTASSIUM in pure methanol. fundamental spectrum. Both the methods This stock solution was used to prepare were found to be simple, accurate, and further standard solutions of the drug. And a economical for the routine analysis of LOSARTAN 100 g/ml (stock solution2) solution was POTASSIUM and its dosage forms. Recovery prepared by dissolving 1ml of standard stock studies were found to be close to 99% that solution in 10ml of methanol. indicated the accuracy and precision of the above two proposed methods. Establishment of Optimal Level of Various Parameters QUANTITATIVE analysis Absorption Maximum Standard stock solution of drug was diluted to %Assay = sample absorbance/standard absorbance x100.

4 Yield different concentrations of 8-22 g/ml. = x100. The absorbance was measured between 200- =98%. standard curve was plotted against concentration versus absorbance of dilutions. The concentration 8-22 g/ml was obeyed Calculations beers law. And square correlation coefficient LOD (LIMIT OF DETECTION). was found to be It is the lowest amount of analyte, in a sample that can be detected. Limit tests merely Market Sample Analysis sustained that the amount of analyte is above Twenty tablets were weighed and powdered or below a certain level..A quantity equivalent to 50mg of LOSARTAN POTASSIUM was weighed accurately transferred DL= into a volumetric flask dissolved in solvent, filtered through whattmann filter paper and LOQ (LIMIT OF QUANTIFICATION). made up to 50ml with solvent. And the It is the lowest concentration of an analyte in amount of LOSARTAN POTASSIUM was found by sample that can be determined with acceptable the calibration curve (10 g dilution of drug).

5 Precision and accuracy. QL=10 Wavelength of Marketed Sample The wavelength of marketed sample of SANDELL'S SENSITIVITY. LOSARTAN POTASSIUM (LOSAR) was found was It is useful to detect the metals present in the found to be Correlation coefficient sample; it is mainly useful for coloured value was found to be compounds Sand ell's sensitivity =molecular weight x no. Recovery Studies To study the accuracy and reproducibility of Of atoms present in molecule/Molar absorpitivity the proposed methods, recovery experiments were carried out by adding a known amount Molar absorpitivity of drug to preanalysed sample and the percentage recovery was calculated. =E1cm1% x molecular weight /10. RESULT AND DISCUSSION. The two simple methods inclusive of simple UV-Spectroscopy and second derivative spectrophotometric methods were developed for the ESTIMATION of LOSARTAN POTASSIUM in pharmaceutical dosage forms. The max of LOSARTAN POTASSIUM was found to be 234nm. Linearity was found to be 8-22 g/ml.

6 Correlation coefficient ( ) indicate good linearity between concentration and slope Fig 1: -max of LOSARTAN POTASSIUM 296. IJRPC 2011, 1(3) Rajesh et al. ISSN: 2231 2781. Table 1. No. P/V Wavelength (nm) Abs 1 Peak Table 2: ABSORBANCE AT 234nm Abs Conc ID Type Wavelength [ug/ml]. (234nm). losartan1. 1 Standard losartan2. 2 Standard losartan3. 3 Standard losartan4. 4 Standard losartan5. 5 Standard losartan6. 6 Standard losartan7. 7 Standard losartan8. 8 Standard Fig 2: Linearity Graph of LOSARTAN POTASSIUM Figure 3: Wavelength of marketed sample 297. IJRPC 2011, 1(3) Rajesh et al. ISSN: 2231 2781. Table3. No. P/V Wavelength (nm) Abs 1 Peak Table 4: ABSORBANCE AT 234nm Abs Conc. No. ID Type (Wavelength [Ug/ml]. nm). LOSARTAN 1 Standard test1. LOSARTAN 2 Standard test2. LOSARTAN 3 Standard test3. LOSARTAN 4 Standard test4. LOSARTAN 5 Standard test5. LOSARTAN 6 Standard test6. LOSARTAN 7 Standard test7. 8 LOSARTAN Standard Figure 4: Linearity Graph of LOSARTAN POTASSIUM 298.

7 IJRPC 2011, 1(3) Rajesh et al. ISSN: 2231 2781. Table 5: ANALYSIS OF LOSARTAN POTASSIUM TABLETS. label amount found Drug %label claim %deviation Claim tab(mg). LOSARTAN 50 100% POTASSIUM 50 50 Table 6: Recovery studies Sample Added amount of drug amount of recovered (mg) recovered (mg ) drug 1 20 2 10 3 10 Figure 5: Second derivative spectrum Table 7. No. P/V Wavelength(nm) Abs 1 Peak 2 Valley 3 - Table 8. Conc( g/ml) Amplitude MPLI. 8 10 12 14 16 18 20 22 299. IJRPC 2011, 1(3) Rajesh et al. ISSN: 2231 2781. LINEARITY GRAPH. 35. 30. Amplitude at 234nm 25. 20. Series2. 15. Series1. 10. 5. 0. 1 2 3 4 5 6 7. Series 1: concentration Series 2: amplitude Fig 6: Linearity Graph Table 9: Analysis of LOSARTAN POTASSIUM Tablets label amount found Drug %label claim %deviation Claim tab(mg). LOSARTAN 50 100% POTASSIUM 50 50 Table 10: Recovery studies Sample Added amount of drug amount of drug recovered (mg) recovered (mg ). 1 20 2 10 3 10 Table 11: Parameters .N Para Parameters LOSARTAN potassiumt 1 max 2 Linearity 8-150 g/ml 3 slope(m) 4 Intercept 5 corelation coefficient 6 Regression equation Y= + 7 Molar absorptivity 8 Sandells sensitivity g/ml 9 Limit of detection g/ml 10 Limit of quantification g/ml 300.

8 IJRPC 2011, 1(3) Rajesh et al. ISSN: 2231 2781. Fig 7: Second derivative spectrum CONCLUSION Blocked By Blood Pressure Drug (in The second derivative spectroscopic method Eng). Science Daily. Retrieved 06-08- of analysis though expensive, can also be 2009. used in the routine analysis of LOSARTAN 3. Guo ZX and Qiu MC. LOSARTAN POTASSIUM in formulations, because multiple downregulates the expression of samples can be analysed simultaneously. The transforming growth factor beta type results obtained by these methods including I and type II receptors in kidney of recovery studies were comparable which diabetic rat. Zhonghua Nei Ke Za proves the repeatability and suitability of the ;42(6):403-08. method 4. , p19. ACKNOWLEDGEMENTS 5. The authors are thankful to /teva-announces-final-approval-of- principal, and V. VENUGOPAL generic-hyzaarr-and-cozaarr-2010-04- Professor of SLC'S college of pharmacy, JNTU 07. Hyderabad and for providing facilities for 6. Rhodes DR, Ateeq B, Cao Q, Tomlins carrying out this work.

9 SA, Mehra R, Laxman B, Kalyana- Sundaram S, Lonigro RJ, Helgeson REFERENCES BE, Bhojani MS, Rehemtulla A, Kleer 1. Habashi JP, Judge DP, Holm TM, CG, Hayes DF, Lucas PC, Varambally Cohn RD, Loeys BL and Cooper TK. S and Chinnaiyan AM. AGTR1. LOSARTAN , an AT1 Antagonist, overexpression defines a subset of Prevents Aortic Aneurysm in a breast cancer and confers sensitivity Mouse Model of Marfan Syndrome, to LOSARTAN , an AGTR1 antagonist. and Preserves Muscle Tissue PNAS. 2009; Architecture in DMD Mouse Models. /cgi/ Science. 2006;312:117-21. 2. University of Michigan Health System. Breast Cancer Gene Can Be 301. IJRPC 2011, 1(3) Rajesh et al. ISSN: 2231 2781. 7. Practical Pharmaceutical Chemistry by Arnold Heyworth Beckett John Bedford Stenlake. Ultra-violet spectroscopy. 275. 302.


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