各種耐性菌に対する - 株式会社 REO研究所

1 Bio Ozone Nano Water ( BON )Bznana B Moe Chou1, 1, 1, 2, 2, 31 2 2 3 REO Bio Ozone Nano Water (BON) Escherichia coli NIHJ JC2 Staphylococcus aureusN315 (MRSA) Staphylococcus aureus:hetero VISAMu3 Staphylococcus aureus:hetero VISA Mu3, VISA;Mu50, HIP PC, HIP NJ, HIP MI (MRSA) Enterococcus faecalisNCTC12201, NCTC12203 (VRE) Enterococcus faeciumNCTC12202, NCTC12204 (VRE) Pseudomonas aeruginosaJICC 3, 4, 5, 6 (MDRP)Staphylococcus aureuspyTEC VCM GM AMK CPFX LVFX IPM CZX CEZABPCTCEM >256Mu3(hetero-VISA) >256 >256 >2563216 >256Mu50(VISA)2565256643212832>256 >2561632 >256 VISA HIP NJ166212832328>2561288025>256 VISA HIP NJ166212832328> >256 VISA HIP >256 VISA HIP MI32612864128816256 > >256 TEC,teicoplanin;VCM, vancomycin; GM, gentamicin;,p ; ,y;,g;AMK, amikacin; CPFX, ciprofloxacin; LVFX, levofloxacin;IPM, imipenem; CZX, ceftizoxim; CEZ, cefazolin; ABPC, ampicillin; ,pee;C,ceo;C,ceo; C,pc;TC, tetracycline.

2 EM, erythromycinEnterococciEnterococciTECVCM GMAMK CPFX LVFXIPMCZXCEZ ATCC > ATCC 19434113212816324> NCTC 12201(VRE)>256 >256 >256>256124>256128232> NCTC12202(VRE)128>25616644864>256 >2563232> NCTC 12203(VRE)>256 >256 >256>2561161>256642128> NCTC 12204(VRE)128>25632644864>256 >2563232>256 TEC,teicoplanin;VCM, vancomycin; GM, gentamicin;TEC,teicoplanin;VCM, vancomycin; GM, gentamicin;AMK, amikacin; CPFX, ciprofloxacin; LVFX, levofloxacin;IPM imipenem; CZX ceftizoxim; CEZ cefazolin; ABPC ampicillin;IPM, imipenem; CZX, ceftizoxim; CEZ, cefazolin; ABPC, ampicillin; TC, tetracycline; EM, erythromycinPseudomonas aeruginosaPseudomonas aeruginosaT E CVCMGMA M KCP FXL VFXI P MCZ XCE ZA BP CT CE M PAO1>256> >256>25616256 JICC3(MDRP)>256>256>256>25664128128>256> 256>25664256 JICC4(MDRP)>256>256>256256256256128>256> 256>25664256 JICC5 (MDRP )>256>256>256>2563264128>256>256>2566425 6 JICC5 (MDRP )>256>256>256>2563264128>256>256>2566425 6 JICC6(MDRP)>256>256>2561283264128>256>25 6>2566425632 g/ml4 g/ml16 g/ml32 g/ml4 g/ml16 g/mlTEC teicoplanin;VCM vancomycin; GM gentamicin;TEC,teicoplanin;VCM, vancomycin; GM, gentamicin;AMK, amikacin; CPFX, ciprofloxacin; LVFX, levofloxacin;IPM imipenem; CZX cefti o im; CEZ cefa olin; ABPC ampicillin;IPM, imipenem; CZX, ceftizoxim; CEZ, cefazolin; ABPC, ampicillin; TC, tetracycline; EM, erythromycin 1.

3 2 O03 2. OD578= 3. 2 1/100, 1/104, and 1/105 ,,4. 50 l 5. 2 1/20 6-2.. 5 HIB BON 6-1. 5 BON ( BON : 40, 60, 80 %) ( BON : 90%) (5x105/ml: S. aureus:based on the standard of CLSI )7151530 7 1, 5, 15 30 8. 100 l 937oC 9. 37oC 10. Escherichia coliNIHJ JC2 Escherichia coli NIHJ JC2 Num. of final 90%515 Time (minutes)Staphylococcus aureusN315 Num. of final 90%1515 Time (minutes)Multi-drug resistantStaphylococcus aureus1000000 Num. of bacteria10000100000Mu3 in BONMu50 in BONVISA HIP PC in BON100010000 VISA HIP PC in BONVISA HIP NJ in BONVISA HIP MI in BONMu3 in DDW10100Mu50 in DDWVISA HIP PC in DDWVISA HIP NJ in DDWVISA HIP MI in DDW1 BON final 90% (minutes)BON final 90%VCM resistant Enterococcus faecalisand Enterococcus faeciumEnterococcus faeciumNum.

4 Of bacteria1000000100001000001124 in BON1125 in BON12201 1220210010001126 in BON1127 in BON1124 in DDW1125 i DDW12203122041220112202101001125 in DDW1126 in DDW1127 in DDW122021220311204 BON final 90% (minutes)01530 Multidrug-resistant Pseudomonas aeruginosaNum. of bacteria100000100000010000100000#3 in BON#4 in BON#5inBONJICC3 JICC4 JICC5 BON1001000#5 in BON#6 in BON#3 in DDW#4inDDWJICC5 JICC6 JICC3 JICC4DW10100#4 in DDW#5 in DDW#6 in DDWJICC4 JICC5 JICC6DW011 BON final 90% (minutes)Effects of medium to bactericidal activity of BON(Ehihi liNIHJ JC2)(Escherichia coli NIHJ JC2)1000000 Num. of bacteria100000 + + + + + + + + + + + + final 60%BON final 40% (minutes)BON final 80%BON final 60%Effects of medium to bactericidal activity of BON(Staphylococcus aureusN315)(Staphylococcus aureusN315)1000000 Num.

5 Of bacteria100000 + + + + + + + + + + + + final 60%BON final 40% (minutes)BON final 80%BON final 60% BON . BON BON