Example: dental hygienist

ReverTra Ace qPCR RT Master Mix

22-05 ReverTra Ace qpcr RT Master Mix (Code No. FSQ-201) TOYOBO CO., LTD. Bioproducts Sales and Marketing Department OSAKA JAPAN A4632K FSQ-201 [1] 1 [2] 2 [3] 3 [4] 4 [5] Appendix 5 [6] 7 [7]

ReverTra Ace ® qPCR RT Master Mix (Code No.FSQ-201) 鋳型: HeLa total RNA 2pg-2μg /20μL 反応系 リアルタイム. PCR . 試薬: THUNDERBIRD. ® SYBR® qPCR Mix (Code No.QPS-201) 鋳型:上記. cDNA2. μL/20μL 反応系(持込量10%) プライマー:代表的な. House Keeping Gene をターゲットとした5 種類 測定:

Tags:

  Master, Thunderbird, Qpcr, Sybr, Revertra ace qpcr rt master mix, Revertra, Revertra ace, 174 qpcr rt master mix, 174 sybr, 174 qpcr mix

Information

Domain:

Source:

Link to this page:

Please notify us if you found a problem with this document:

Other abuse

Transcription of ReverTra Ace qPCR RT Master Mix

1 22-05 ReverTra Ace qpcr RT Master Mix (Code No. FSQ-201) TOYOBO CO., LTD. Bioproducts Sales and Marketing Department OSAKA JAPAN A4632K FSQ-201 [1] 1 [2] 2 [3] 3 [4] 4 [5] Appendix 5 [6] 7 [7]

2 8 - 1 - FSQ-201 [1] ReverTra Ace qpcr RT Master Mix ReverTra Ace PCR ReverTra Ace RNase Inhibitor Oligo dT Primer Random Primer dNTPs buffer 5 RNA PCR cDNA PCR cDNA PCR PCR PCR thunderbird qpcr Mix Realtime PCR Master Mix ([7] ) 1.

3 -20 C RNA cDNA no-RT Control 2. RNA Oligo dT Random Primer RNA 3. PCR cDNA 15 PCR RNA RNase H ( ) 4. PCR PCR 20% PCR mRNA FSQ-201 - 2 - [2]

4 10 L 200 10 L 20 5 RT Master Mix no-RT Control -20 C 5 RT Master Mix -20 C 400 L 5 RT Master Mix no-RT Control -20 C 40 L Nuclease-free Water -20 C 1000 L x2 5 RT Master Mix ReverTra Ace RNase inhibitor Oligo dT Primer Random Primer MgCl2 dNTPs 5 5 RT Master Mix no-RT Control 5 RT Master Mix ReverTra Ace 5 RT Master Mix Nuclease-free Water Nuclease-free (DEPC) 1 -30 C 2 5 RT Master Mix 5 RT Master Mix no-RT Control ReverTra Ace qpcr RT Master Mix with gDNA Remover (Code No.)

5 FSQ-301) 3 Random Primer Oligo dT Primer (Gene Specific Primer) ReverTra Ace qpcr RT Kit (Code ) - 3 - FSQ-201 [3] (37 C 50 C 65 C 98 C) Nuclease-free Water 200 RNA DEPC( ) Nuclease-free Water DEPC DEPC DEPC PCR Nuclease-free Water Total RNA Total RNA Total RNA mRNA 1-2% Total RNA RNA AGPC(Acid Guanidium - Phenol - Chloroform)

6 Total RNA DNA DNA DNase I DNA DNA ReverTra Ace qpcr RT Master Mix with gDNA Remover(Code No. FSQ-301) poly(A)+ RNA (mRNA) poly(A)+ RNA oligo(dT) poly(A)+ mRNA mRNA mRNA Total RNA (RNase) ribosomal RNA FSQ-201 - 4 - [4] (1) RNA * RNA 65 C 5 * RNA 5 RT Master Mix (2)

7 5 RT Master Mix 2 L RNA template 1pg 1 g Nuclease-free Water Total 10 L 5 RT Master Mix 5 RT Master Mix no-RT Control cDNA (3) 37 C, 15min. 50 C, 5min. ** 98 C, 5min. 4 C, hold ** ReverTra Ace 4 C -20 C PCR PCR 20% PCR - 5 - FSQ-201 [5] Appendix A.

8 CDNA ReverTra Ace qpcr RT Master Mix (Code ) HeLa total RNA 2pg-2 g /20 L PCR thunderbird sybr qpcr Mix (Code ) cDNA2 L/20 L ( 10%) House Keeping Gene 5 Applied Biosystems 7900HT 5 101214161820222426283032343601234567 LOG RNA CtATP5 FTFRCRPLP1 RPLP2 RPS18 (TFRC) (RPLP1) (ATP5F) (RPLP2) (RPS18) , , , ,000, ,000, lS l o p e- 3.

9 2 8 0- 3 . 3 0 3- 3 . 3 8 4- 3 . 2 8 4- 3 . 3 6 8R20 . 9 9 90 . 9 9 91 . 0 0 01 . 0 0 00 . 9 9 9E f f .1 0 1 . 8 %1 0 0 . 8 %9 7 . 5 %1 0 1 . 6 %9 8 . 1 % qpcr Target Ct TemplateRNA (pg)Log(RNA )FSQ-201 - 6 - B. Total RNA DNase I AGPC Total RNA DNA DNA ( ) DNA DNA ReverTra Ace qpcr RT Master Mix with gDNA Remover (Code No.)

10 FSQ-301) (1) ( ) Total RNA (<10 g) X L 10x DNase I Buffer 1 L (10mM Tris-Cl, , MgCl2, CaCl2) RNase-free DNase I (10U/ L) L Nuclease-free Water to 10 L 10 30 (2) ( ) 100 L Nuclease-free Water 100 L TE 5 12,000rpm 5 100 L 12,000rpm 5 100 L 5M 200 L -20 C 30 12,000rpm 5 70% 12,000rpm 5 Nuclease-free Water - 7 - FSQ-201 [6]