RIPA 蛋白提取液、蛋白酶及磷酸酶抑制剂

1 RIPA Cat. No.: C1901 C2501 S0142 S0143 C1901 Co-IP/IP RIPA Lysis buffer Co-IP/IP RIPA Lysis buffer 100ml RT Co-IP/IP C2501 WB Super RIPA Lysis buffer WB Super RIPA Lysis buffer 100ml RT Western Blot Co-IP/IP S0142 A 100X A 100X 100 l -20 C S0143 ABC 100X A 100X 100 l -20 C B 100X 100 l -20 C C 100X 100 l -20 C A B C 104mM AEBSF 80 M Aprotinin 5mM Bestatin E-64 2mM Leupeptin Pepstatin A 100mM Sodium Fluoride 100mM Sodium Orthovanadate 400mM Sodium Tartrate 115mM Sodium Molybdate 200mM Imidazole (-)-p-Bromotetramisole oxalate 500 M Cantharidin 500nM Microcystin LR, Microcystis aeruginosa Serine Aminopeptidases Cysteine Aspartic proteases Acid phosphatases Alkaline phosphatases PTPs, ATPases, Acid phosphatases Acid and phosphoprotein Alkaline phosphatases Alkaline phosphatases Ser/Thr phosphatases PP1 and PP2A Western Blot C2501 S0142 Western Blot C2501 S0143 Co-IP/IP C1901 S0142 Co-IP/IP C1901 S0143 Web: 400-0470-600 Email: RIPA Western Blot 1.

2 10 106 (1). A: WB Super RIPA A (S0142) 1 250 l WB Super RIPA l A B: WB Super RIPA A B C (S0143) l 1 250 l WB Super RIPA l A l B l C WB Super RIPA -20 C 2 Benzonase C2001 U/ l (2). 250 l 30min (3). 13,000 rpm 5min 2. (4). (5). 10 mg 250 l ( ) 30min (6). 13,000 rpm 5min (7). BCA (HaiGene,C3001) Benzonase NanoDrop Co-IP/IP 1. 10 106 (1). A: Co-IP/IP RIPA A (S0142) 1 250 l Co-IP/IP RIPA l A B: Co-IP/IP RIPA A B C (S0143) l 1 250 l Co-IP/IP RIPA l A l B l C Co-IP/IP RIPA -20 C 2 (2).

3 250 l 30min (3). 13,000 rpm 5min 2. (4). (5). 10 mg 250 l ( ) 30min (6). 13,000 rpm 5min (7). BCA (HaiGene,C3001) Benzonase NanoDrop Web: 400-0470-600 Email: Web: 400-0470-600 Email.