乳癌の内分泌療法抵抗性 ... - saitama-med.ac.jp

1 23 36 1 21 9 1) 2) 1) ER ER (ERE) 2) ER ER (ER) (PR) ER PR 48 ER (ERR ) 55 3) ER FOX ER (AR)

2 4) ER 121 C 10 ER ERR ER (Envision, DAKO ) ER MCF-7 (10- 6M) DMEM 2 (Tam-R) MCF-7 DMEM 4 (Long Term Estrogen Deprived, 19 20 1 ) 2 )24 LTED) Tam-R SF( )

3 4000 24 well-plate 24 72 120 WST-8 10 l 2 37 C 450 nm Tam-R SDS-PAGE ER Cell Signaling Technology ER Tam-R LTED mRNA RNA cDNA PCR ABI PRISM 7000, ER 2004 11 2007 7 20 20 57 89 77 I III 18 IV 2 ER PR 13 ER PR 7 2 3 15 ERR 12 (60 ) ERR MCF-7 (10- 6M) 2 (Tam-R) 18 6 (WST- 8 aasay)

4 MCF-7 ER Tam-R MCF-7 PCR ER mRNA Tam-R ER ER mRNA Tam-R (PR) Tam-R (GR) PR GR 5) Tam-R Forkhead box (FOX) 4) FOXA1 ER (AR) ER Tam-R FOXA1 MCF-7 (LTED)

5 LTED ER ER FOXA1 ER FOXA1 Tam-R MCF-7 ER Tam-R LTED ER Tam-R LTED ER ER PR GR FOX FOX 25 1. T a k a y a m a K , K a n e s h i r o K , T s u t s u m i S , Horie-Inoue K, Ikeda K, Urano T, Ijichi N, Ouchi Y, Shirahige K, Aburatani H, Inoue S. Identification of novel androgen response genes in prostate cancer cells by coupling chromatin immunoprecipitation a n d g e n o m i c m i c r o a r r a y a n a l y s i s.

6 O n c o g e n e 2007;26 Ijichi N, Ikeda K, Horie-Inoue K, Yagi K, Okazaki Y, Inoue S. Estrogen-related receptor modulates the expression of adipogenesis-related genes during adipocyte dif ferentiation. Biochem Biophys Res Commun 2007;358 Ichikawa T, Horie-Inoue K, Ikeda K, Blumberg B, Inoue S. Vitamin K2 induces phosphor ylation of protein kinase A and expression of novel target genes in osteoblastic cells. J Mol Endocrinol 2007;39 Kubo M, Ijichi N, Ikeda K, Horie-Inoue K, Takeda S, Inoue S. Modulation of adipogenesis-related gene expression by estrogen-related receptor during adipocytic dif ferentiation. Biochim Biophys Acta 2009;1789 Takayama K, Horie-Inoue K, Ikeda K, Urano T, Murakami K, Hayashizaki Y, Ouchi Y, Inoue S.

7 FOXP1 is an androgen-responsive transcription factor that negatively regulates androgen receptor signaling in prostate cancer cells. Biochem Biophys Res Commun 2008;374 T a k a y a m a K , T s u t s u m i S , S u z u k i T , Horie-Inoue K, Ikeda K, Kaneshiro K, Fujimura T, Kumagai J, Urano T, Sakaki Y, Shirahige K, Sasano H, Takahashi S, Kitamura T, Ouchi Y, Aburatani H, Inoue S. Amyloid precursor protein is a primar y androgen target gene that promotes prostate cancer growth. Cancer Res 2009;69 T a k e o C , I k e d a K, H o r i e - I n o u e K , I n o u e S. Identification of Igf2, Igfbp2 and Enpp2 as Estrogen-Responsive Genes in Rat Hippocampus. Endocrine J 2009;56:113-20.

8 1. TRIM5 Efp (TRIM25) 55 2007 10 21-23 2. 28 2007 10 19-20 3. 15 2007 11 23-24 4. Nobuhiro Ijichi, Mayumi Kubo, Kazuhiro Ikeda, Kuniko Horie-Inoue, and Satoshi Inoue. Functional role of estrogen-related receptor family in adipocyte differentiation 13 20 8 22 5. Nobuhiro Ijichi, Kazuhiro Ikeda, Kayoko Murakami, Kuniko Horie-Inoue, Jun Kawai, Yoshihide Hayashizaki, Satoshi Inoue. Gene expression profiles of nuclear hormone receptors regulated by estrogen in human breast cancer MCF-7 cells The 26th Congress of the International Association for Breast Cancer R e s e a r c h S e p t e m b e r 2 2 - 2 4 , 2 0 0 8 , K u r a s h i k i , Okayama, Japan6.

9 ERR 29 2008 10 17-18 7. Kazuhiro Ikeda, Nobuhiro Ijichi, Mayumi Kubo, Kuniko Horie-Inoue, Satoshi Inoue. Estrogen-related receptors in fats and adipogenesis. CBI Annual Meeting 2008 Inter national Symposium August 22-24, 2008 Tokyo8. EPAS1/HIF2 16 2008 11 22 9. Efp 16 2008 11 22 10. MCF7 BMB2008 31 81 2008 12 9-12 11. Glutamate receptor subunit 2D (Grin2d) DECODE 2009 1 19-21 26 2009 The Medical Society of Saitama Medical , , , , Efp 9 2009 1 24 13.

10 9 2009 1 24 1) Huang WY, Newman B, Millikan RC, Schell MJ, Hulka BS, Moorman PG. Hormone-related Factors and Risk of Breast Cancer in Relation to Estrogen Receptor and Progesterone Receptor Status. Am J Epidemiol 2000;151 ) Lone RO, Frith MC, Karlsson EK, HansenRosen U. Mol Endocrinol 2004;18 ) Suzuki T, Miki Y, Moriya T, Shimada N, Ishida T, Hirakawa H, Ohuchi N, Sasano H. Estrogen-related receptor alpha in human breast carcinoma as a potent prognostic factor. Cancer Res 2004;64:4670-6. 4) Carroll JS, Meyer CA, Song J, Li W, Geistlinger TR, Eeckhoute J, Brodsky AS, Keeton EK, Fertuck KC, Hall GF, Wang Q, Bekiranov S, Sementchenko V, Fox EA, Silver PA, Gingeras TR, Liu XS, Brown M.