SPECIMEN COLLECTION MANUAL

1 Western Diagnostic Pathology Copyright - Copying is Not Permitted Title: Revision: 2 Document Number: Page 1 of 3 Authorisation: SPECIMEN COLLECTION MANUAL Issue 19 FRM-COLL-71 Customer Experience Manager SPECIMEN COLLECTION MANUAL ISSUE 19 Copy Number: COLLECTION Centre Name: Western Diagnostic Pathology Copyright - Copying is Not Permitted Title: Revision: 2 Document Number: Page 2 of 3 Authorisation: SPECIMEN COLLECTION MANUAL Issue 19 FRM-COLL-71 Customer Experience Manager SPECIMEN COLLECTION MANUAL Table of Contents Document Number Document Name Revision FRM-COLL-71 Cover Page, Table of Contents and Condition of Issue 2 Guides FRM-COLL-47 Order of Draw and Tube Guide 3 FRM-COLL-109 SPECIMEN Bag Guide 1 FRM-COLL-110 COLLECTION Sticker Guide 1 FRM-COLL-86 Coagulation COLLECTION Guide 5 FRM-COLL-108 24 hour urine Guide 3 FRM-COLL-81 Swab COLLECTION Guide 4 FRM-COLL-80 Urine and Genital Swab COLLECTION Guide 5 FRM-COLL-90 Helicobacter Restricted Medication List 2 Out of Pocket (OOP) FeesFRM-COLL-104 Out of Pocket Guide 5 Work Instructions (WI) WI-COLL-49 Quantiferon Gold Test for TB Immunity 5 WI-COLL-83 Eye Swab - COLLECTION Instruction 6 COLLECTION MANUAL A- Z Western Diagnostic Pathology Copyright - Copying is Not Permitted Title: Revision: 2 Document Number: Page 3 of 3 Authorisation.

2 SPECIMEN COLLECTION MANUAL Issue 19 FRM-COLL-71 Customer Experience Manager Conditions of Issue The SPECIMEN COLLECTION MANUAL is a controlled MANUAL under the Western DiagnosticPathology (WDP) Management System. This MANUAL is not to be removed from its designated location. If it is moved from itsdesignated location, it is considered an uncontrolled MANUAL . Uncontrolled manuals will not receive updates. The information in an uncontrolled manualcannot be relied upon as current or accurate. Copying or partial copying of this MANUAL is prohibited and in breach of Copyright and NATA regulations. This MANUAL is issued by WDP for use by staff, affiliated phlebotomists and clients. It alwaysremains the property of WDP. This MANUAL is not to be marked or amended in any manner except when instructed by theDocument Controller. This includes using post-it notes for any reason except as a pageindicator. If you notice any incorrect or inconsistent information, please contact your supervisor or theCustomer Experience Manger (phone 08 9317 0999) as soon as possible.

3 The MANUAL is to be updated in a timely manner when a notification of change memo isreceived. In the event of the MANUAL being lost, damaged or destroyed the Document Controller is tobe notified as soon as possible. A further copy may be issued at the discretion of WDP. If this MANUAL is found, please return it to the designated location on the front cover or theCustomer Experience Manger at Western Diagnostic Pathology, 74 McCoy St, Myaree : Draw Order and Tube Guide Revision:3 Document Number: FRM-COLL-47 Page 1 of 1 Authorisation: Customer Experience Manager Order of Draw & Tube Guide Tube Tube Name Instruction Adult Paediatric Blood Culture Refer to Blood Culture COLLECTION (WI-COLL-74) 10mL Streck Tube Refer to Generation NIPT Blood COLLECTION (WI-COLL-79) Quantiferon Gold Set Refer to Quantiferon Gold Test for TB Immunity (WI-COLL-49) Sodium Citrate Tubes must be full draw volume 6mL ACD SST Let tube clot for 30 minutes prior to centrifuging 6mL Plain Tube or 6mL 2mL Lithium Heparin (No Gel) PST (with gel) Tubes must be a full draw volume 6mL Trace Element Free 4mL 2mL EDTA If ESR requested tube must be full draw volume 6mL EDTA 6ml - Pink Tubes and declaration on request form to be signed by collector 6mL PPT 2mL Fluoride Oxalate (FLOX) NOTE: All tubes to be gently Inverted 8-10 times.

4 HAZARD WARNING: Never inject blood into the tube from needle and syringe. Always label tubes legibly Surname, Given Name, Time and Date of COLLECTION , Date of Birth and Collector s Signature. Do not transfer blood from tube to tube to prevent contamination of additives. Title: SPECIMEN Bag Guide Revision:1 Document Number: FRM-COLL-109 Page 1 of 1 Authorisation: Customer Experience Manager SPECIMEN Bag Guide SPECIMEN Bag Red Clear Purple Instruction All Urgent Samples Routine Samples Craigie IVF SPECIMEN Bag Green Blue Orange Instruction Attention SPECIMEN Reception Supervisors Unspun specimens Nursing Homes/Care facility samples Non-Urgent Sodium Citrate Samples and other samples collected for the particular patient Holter Monitor Title: COLLECTION Sticker Guide Revision:1 Document Number: FRM-COLL-110 Page 1 of 1 Authorisation: Customer Experience Manager COLLECTION Sticker Guide Sticker Instruction Specimens are deemed urgent only by the requesting doctor or SPECIMEN COLLECTION MANUAL .

5 Please ensure that you complete all relevant fields on the sticker, write n/a if the patient is not having operation or chemotherapy. All forms must be scanned/faxed through to the urgent queue +/- phone the testing laboratory or the Laboratory Liaison Officers depending on the urgency of the test as per the Processing Urgent SPECIMEN Protocol (PRC-ADM-1). Where the Doctor has indicated on the request forms that the results are confidential. Only the Doctor may make this decision. For indicating samples to be frozen on the tubes and request forms (this assist SRA that frozen sample(s) to follow). Provide the photocopy of the request form when sending frozen samples. For indicating samples to be kept unspun due to analytical requirements. For indicating samples to be kept unopened until testing. Test names: Blood Alcohol Ionised Calcium Hep B Viral Load Hep C Viral Load Title: Coagulation COLLECTION Guide Revision:5 Document Number: FRM-COLL-86 Page 1 of 2 Authorisation: Senior Scientist - Haematology Coagulation COLLECTION Guide If you calculate that you will collect more than 5 citrate tubes, have a platelet aggregation studies or have any other queries please ring Coagulation on 9317 0862, 9317 0861 or 9317 0863.

6 Please note tests marked as $ incur out of pocket expense the patient must be informed. If the transport time limit cannot be achieved, the samples can be spun and frozen as per the last column. Single or Double spinning is to be performed as per WI-COLL-40. Remember to label the tubes with S/S or D/S to indicate whether the sample was single or double spun. Frozen samples should be transported on dry ice wherever possible. For areas with shorter transit times, alternatives are acceptable so long as the samples remain frozen on receipt at the main laboratory. Questionnaires: Patient to complete Coagulation Questionnaire (FRM-COLL-43) for any coagulation testing. Remember to Lab Number this form & fax it as well as the request form. If the patient is being tested for Thrombophilia, Prothrombin gene or FV Leiden, the Genetics Tests Questionnaire (FRM-COLL-31) should also be completed. Remember to Lab Number this form & fax it as well as the request form.

7 Coagulation Collections Guide Table: Screen requested Individually requested tests TUBES Special instructions LIMIT (HRS) Citrate Spinning COAGS, CLOTTING PROFILE, COAG SCREEN (includes all tests below) 2 CIT 10 Single spin INR, PT, Prothrombin Time 1 CIT 24 Single APTT, PTT, PTTK 1 CIT 10 Single Fibrinogen, FIB 1 CIT 10 Single TCT, TT, REPTILASE 1 CIT 10 Single D-DIMER, DD, FDP 1 CIT Always treat as urgent 4 Single spin THROMBOPHILIA PROCOAGULANT, PROTHROMBOTIC SCREEN $ (includes all tests below) 4 CIT 1 PPT 2 EDTA 1 SST PPT - spin within 30 min EDTA x 1 - Molecular Genetics Sticker 1 SST - is for ACL Citrates- if >10hrs, double spin/separate/freeze. 10 Double spin ATIII (antithrombin) 2 CIT 10 Single Protein C $ 2 CIT Patient must not be on warfarin 10 Single Protein S $ 2 CIT Patient must not be on warfarin 10 Single FVL (factor V Leiden) $ 2 CIT 1 EDTA 1 EDTA - Molecular Genetics Sticker 10 Single Title: Coagulation COLLECTION Guide Revision:5 Document Number: FRM-COLL-86 Page 2 of 2 Authorisation: Senior Scientist - Haematology **Any combination of ALL tests MAXIMUM 9 citrates (+/- other tube types) ** Prothrombin Gene (PGM, G20210A, PT Gene) $ 1 EDTA 1 EDTA - Molecular Genetics Sticker 10 Single aPCR (activated protein C resistance) $ 2 CIT 1 EDTA 1 EDTA - Molecular Genetics Sticker 10 Single Lupus anticoagulant (LAC) 2 CIT If >10 hrs, double spin/ separate/ freeze 10 Double Antiphospholipid Abs (LAC/ACL) 2 CIT 1 SST 1S- for ACL If more than 10 hours, double spin/ separate/ freeze Citrates.

8 10 Double Homocysteine (random) 1 PPT PPT- spin within 30 minutes - N/A Anticardiolipin &/or B2GP1 Ab s 1 SST - N/A vWF, vWD, CLOTTING FACTORS, FACTOR STUDIES, BLEEDING SCREEN, FVIIIc, HAEMOPHILIA SCREEN (includes tests below) 3 CIT (max) If more than 10 hours, spin/separate/freeze. 10 Single spin FVIIIc 3 CIT 10 Single vWF:Ag 3 CIT 10 Single Ristocetin Cofactor (vWF Activity, Ricof) 3 CIT 10 Single CBA 1 CIT 10 Single FIX, FXI, FXII 2 CIT 10 Single PFA-100, PFA-200, PLATELET FUNCTION, BLEEDING TIME 2 CIT DO NOT SPIN stickers; Keep at Room temp. 4 DO NOT SPIN **Any combination of tests above MAXIMUM 5 citrates (+/- other tube types)** PLATELET AGGREGATION, PAG +/- mepacrine staining 5 CIT Phone MYA Coags BEFORE COLLECTION . DO NOT SPIN stickers; Room temp. Collect before 3pm M-F To Myaree within 1 hour 1 DO NOT SPIN OTHER SPECIAL TESTS 4 CIT (max) - CAT 2 CIT 4 Double Anti-Xa, for Rivaroxaban (Xeralto), Apixaban (Eliquis) or Heparin (Clexane, Fragmin) 1 CIT Record drug name and date/ time of last dose on form.

9 4 Double Anti-Platelet antibodies 2 CIT 1 SST Citrates - DO NOT SPIN stickers; Keep at room temp; To FSH within 36hrs. 36 DO NOT SPIN HITT Screening 4 CIT 1 PLAIN To FSH within 4hrs or single spin/ freeze. Must notify FSH before COLLECTION . Submit vial of heparin same lot as used. 4 Single Platelet microparticles/ Zymuphen microparticle activity 1 CIT To FSH within 4hrs or single spin/ freeze. 4 Single Factor Inhibitors, Inhibitor Screen, FII, FV, FVII, FX, FXIII, ADAMTS-13, XACT, PAI-1, PF1 & 2, TAT, Plasma phospholipids 1 CIT each, max 3 4 Double Title: 24- hour Urine Guide Revision:3 Document Number: FRM-COLL-108 Page 1 of 1 Authorisation: Chief Scientist Automated Biochemistry 24 hour urine Guide TEST SAMPLE TYPE MORE INFORMATION 5 HIAA (Hydroxy Indole Acetic Acid) WITH ACID Patient must be on serotonin-free diet two days prior to and during urine COLLECTION . Avoid plums, avocado, bananas, pineapple, avocado, kiwi fruit, nuts, chocolate and paracetamol.

10 Albumin Excretion PLAIN Overnight/timed or 24 hour COLLECTION . Note start and finish time on form. Amylase Clearance PLAIN Calcium Excretion (CAE) WITH ACID Catecholamines/Metanephrines WITH ACID Creatinine Clearance PLAIN SST tube to be collected within 24 hours of the urine COLLECTION when patient drops off completed sample. Citrate WITH ACID If citrate/creatinine ratio is requested, collect a spot urine. Urine Free Cortisol PLAIN If a dexamethasone suppression test is requested at the same time, collect this test first and schedule the dexamethasone suppression test for when the urine is returned to the COLLECTION centre. Cystine PLAIN If cystine screen is requested, collect a spot urine. Oxalate WITH ACID If oxalate/creatinine ratio is requested, collect a spot urine. Always collect 24- hour urine for above tests and provide 2 container each for sample type TEST SAMPLE TYPE MORE INFORMATION Bence Jones Protein/ BJP/ IEP * PLAIN *Collect 24 hour urine only when indicated otherwise collect a spot urine with NO ACID Calcium* WITH ACID Calcium Creatinine Ratio* PLAIN Chloride PLAIN Creatinine ACID or PLAIN Magnesium* WITH ACID Microalbumin* PLAIN Phosphate* WITH ACID Potassium* PLAIN Protein Creatinine Ratio* PLAIN Sodium* PLAIN Urea* PLAIN Uric Acid* PLAIN Urine electrophoresis* PLAIN Title: Swab Guide Revision: 4 Document Number: FRM-COLL-81 Page 1 of 1 Authorisation: Senior Scientist Molecular Infectious Chief Scientist Microbiology Swab COLLECTION Guide TEST SITE DR Doctor collect PT Patient collect SWAB Storage Condition.