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SPECIMEN COLLECTION OF NASOPHARYNGEAL SWAB AND ...

SPECIMEN COLLECTION OF NASOPHARYNGEAL SWAB AND oropharyngeal SWABRESEARCH INSTITUTE FOR TROPICAL MEDICINESUPPLIES/EQUIPMENT NEEDED COVID-19 Case Investigation Form (CIF) and RITM linelist Virus Transport Media (VTM) or Universal Transport Media (UTM) or Sample Storage Reagent (Sansure) NASOPHARYNGEAL swab, Sterile Dacron/Rayon swab with pliable shaft Oropharyngealswab, Sterile Dacron/Rayon swab with plastic shaft Sterile tongue depressor Test Tube rack Resealable plastic bags (zip lock) Laboratory sealing film (parafilm) Masking tape Permanent tube marker Scissors PPE (Laboratory gown, gloves, N95mask, goggles) RefrigeratorRESEARCH INSTITUTE FOR TROPICAL MEDICINECOLLECTION KIT Virus Transport Medium NASOPHARYNGEAL swab oropharyngeal swabCOPAN SAMPLE COLLECTION KIT Universal Transport Medium (UTM) or equivalent Copan Flocked Swab or equivalentbreakpointSample Storage Reagent (Sansure) and OPS 5 RESEARCH INSTITUTE FOR TROPICAL MEDICINEDIRECTIONSFORUSEOFSWAB Swabsshouldnotbeusedif thereisevidenceofdamageorcontamination theexpirationdatehaspassed theswabpackageisdamaged thereareothersignsofdeterioration Duringsampling,theswabshallonlycomeincon tactwiththesuspectedinfection.

•Oropharyngeal swab, Sterile Dacron/Rayon swab with plastic shaft •Sterile tongue depressor •Test Tube rack •Resealable plastic bags (zip lock) •Laboratory sealing film (parafilm) •Masking tape •Permanent tube marker •Scissors •PPE (Laboratory …

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Transcription of SPECIMEN COLLECTION OF NASOPHARYNGEAL SWAB AND ...

1 SPECIMEN COLLECTION OF NASOPHARYNGEAL SWAB AND oropharyngeal SWABRESEARCH INSTITUTE FOR TROPICAL MEDICINESUPPLIES/EQUIPMENT NEEDED COVID-19 Case Investigation Form (CIF) and RITM linelist Virus Transport Media (VTM) or Universal Transport Media (UTM) or Sample Storage Reagent (Sansure) NASOPHARYNGEAL swab, Sterile Dacron/Rayon swab with pliable shaft Oropharyngealswab, Sterile Dacron/Rayon swab with plastic shaft Sterile tongue depressor Test Tube rack Resealable plastic bags (zip lock) Laboratory sealing film (parafilm) Masking tape Permanent tube marker Scissors PPE (Laboratory gown, gloves, N95mask, goggles) RefrigeratorRESEARCH INSTITUTE FOR TROPICAL MEDICINECOLLECTION KIT Virus Transport Medium NASOPHARYNGEAL swab oropharyngeal swabCOPAN SAMPLE COLLECTION KIT Universal Transport Medium (UTM) or equivalent Copan Flocked Swab or equivalentbreakpointSample Storage Reagent (Sansure) and OPS 5 RESEARCH INSTITUTE FOR TROPICAL MEDICINEDIRECTIONSFORUSEOFSWAB Swabsshouldnotbeusedif thereisevidenceofdamageorcontamination theexpirationdatehaspassed theswabpackageisdamaged thereareothersignsofdeterioration Duringsampling,theswabshallonlycomeincon tactwiththesuspectedinfection.

2 Donotuseexcessiveforcewhencollectingswab samplesfrompatientsasthismayresultinacci dentalbreakageoftheswabshaft. INSTITUTE FOR TROPICAL :THERESPIRATORYSYSTEMRESEARCH INSTITUTE FOR TROPICAL MEDICINEAREAFORNASOPHARYNGEALSWABSAMPLIN GTARGETSITEFORNPSRESEARCH INSTITUTE FOR TROPICAL MEDICINETHEORALCAVITYS ensitivetogagreflexTARGETSITEFOROPST ogainaccesstotheoralcavity,useatonguedep ressorGUIDELINESPRIORTOSPECIMENCOLLECTIO NINFECTIONCONTROLGUIDELINES Personalprotectiveequipment:wearN95maska nddisposablegloves. Whencompleted,disposeofallPPEandothercon taminatedmaterialsintheappropriatetrashb in. Washhandsthoroughlywithsoap and wateror alcohol basedhandgelbefore AND after INSTITUTE FOR TROPICAL MEDICINESPECIMENCOLLECTIONPOLICIES Specimensshallbecollectedwithin10daysfro monsetofillness. Onlyqualifiedandtrainedstaffshall performthe procedures. DoNOTusewoodenandcottonswabs. CheckforintegrityoftheOPS/NPSswabsanddon ot usebeyondexpiration.

3 CorrectlyidentifythepatientandlabeltheUT Mtubepriortocollection. Labelthetubewiththepatient sfullname, Removepossiblevisualobstructions. Strictlyfollowinfectioncontrolguidelines priortoeachprocedure.(Protectyourselffro mthepatient)SPECIMENCOLLECTIONPROPERNASO PHARYNGEALSWAB(NPS)RESEARCH INSTITUTE FOR TROPICAL MEDICINE Usingtheswab,visuallymeasure*fromthebase ofthenostriltowardstheauditorypit. Dividethelengthintohalfinordertoknowinto whatextentwillbeinsertedintothenostril(u sually5 6cminadults)toensurethatitreachesthepost eriorpharynx.*Alternatively,youmayusearu lerformoreaccuratemeasurementsRESEARCH INSTITUTE FOR TROPICAL MEDICINENPS:STEP2 Withthepatientseated, :STEP3 Inserttheswabintothenasalcavityuntilasli ghtresistanceismet. Rotatetheswabandapplyalittleforcetotakel argequantitiesofmucosa RepeatintheothernostrilusingsameswabRESE ARCH INSTITUTE FOR TROPICAL MEDICINENPS:STEP4 PlacetheNPSimmediatelyintheUTMtubetoavoi ddryingoftheswab.

4 Break/Cuttheendoftheshaftthatsticksoutof thetube(breakpoint)andclosethetubetightl y. SecurethecapwithParafilmtopreventleakage duringtransportNPS:STEP5 Transportthespecimentothelaboratoryandim mediatelystoreinsiderefrigerator(2-8C). Ifsiteisfarfromarefrigerator,useathermob ag/boxwith4-6icepacksRESEARCH INSTITUTE FOR TROPICAL MEDICINESPECIMENCOLLECTIONPROPEROROPHARY NGEALSWAB(OPS)OPS:STEP1 Havethepatientseatedcomfortably. Havethepatientopenhismouth. Withglovedhands,holddownthetonguewithast eriletonguedepressor Havethepatientsay AAH to elevate theuvulaOPS:STEP2 Useasweepingmotiontoswabtheposteriorphar yngealwallandtonsillarfossa. Avoidswabbingthesoftpalate. :STEP4 PlacetheOPSimmediatelyinthesameUTMwithNP S. Cuttheendoftheshaftthatsticksoutofthetub eandclosethetubetightly. INSTITUTE FOR TROPICAL MEDICINEBESTPRACTICES Askpatienttoremoveextrasecretionsbeforep rocedure. Askpatienttoclosehisorhereyes.

5 Visuallyestimatethedepthofinsertion. LabelthetubefirstPRIOR tocollection. INSTITUTE FOR TROPICAL MEDICINESPECIMENCOLLECTIONPOLICIESS ample Storage Reagent(Sansure)Room temperature or 2-8C

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