1 Quest DiagnosticsProper Phlebotomy TechniquesCollectMix GentlySeparateThe stopper color alone does not indicate tube type also refer to the tube gentle inversionsunless otherwise notedWhen using a winged blood Collection set for venipuncture and a coagulation (citrate) tube is thefirst Specimen to be drawn, a discard tube should be drawn first. The discard tube must be used tofill the blood Collection set tubing s dead space with blood but the discard tube does not need tobe completely filled. This important step will ensure maintenance of the proper blood-to-additiveratio of the blood Specimen . The discard tube should be a nonadditive or coagulation tube. RED/BLACKREDROYAL BLUEGREENGRAY, andWhite LabelTANYELLOW, andWhite Label withYellow Horizontal StripeYELLOW (CULTURE), andNo Paper Label (adult tube) orYellow Label(pediatric tube)LAVENDERLIGHT BLUEGRAY, andYellow LabelYELLOW PLASTIC, andYellow LabelYELLOW (CULTURE)LIGHT BLUEYELLOWRED/BLACKREDGREENLAVENDER GRAYTUBES WITH OTHER ADDITIVES Clot activator and gel for serum separation5 Inversions Clot activator5 Inversions (plastic)None (glass) Sodium heparin Na2 EDTA None (serum tube) Sodium heparin Lithium heparin Potassium oxalate/sodium fluoride Sodium fluoride/Na2 EDTA Sodium heparin (glass) K2 EDTA (plastic) Acid Citrate Dextrose(ACD).
2 Solutions A/B additives Trisodium citrate , citric acid and dextrose (in g/L) Sodium Polyanethol Sulfonate (SPS) in Sodium Chloride Spray-dried K2 EDTA M sodium citrate ( ) 4 Inversions Boric acid, sodium formateShake vigorously PreservativeSerum Separator Tube (SST ) for serum determinations inchemistry and serology. Contains separator gel and should notbe used for toxicology or drug testing. Inversions ensure mixing of clotactivator with blood. Blood clotting time 30 serum determinations in chemistry and serology, and fortoxicology and drug testing. Glass serum Tubes are recommendedfor blood banking. Plastic Tubes contain clot activator and arenotrecommended for blood banking. Inversions ensure mixingofclot activator with blood and clotting within 60 trace-element, toxicology and nutritional-chemistrydeterminations. Special stopper formulation provides low levels of trace plasma determinations in chemistry.
3 Tube inversionsprevent glucose determinations. Oxalate and EDTA anticoagulants will give plasma samples. Sodium fluoride is the antiglycolytic agent. Inversions ensure proper mixing of additive and lead determinations. This tube is certified to contain less than g/mL (ppm) lead. Inversions prevent tube with liquid ACD for use in blood bank studies, HLA phenotyping, DNA, paternity testing, tube with liquid SPS anticoagulant for mycobacterial (tuberculosis) and fungus blood for whole blood hematology determinations and immunohematology testing (ABO grouping, Rh typing, antibody screening). Inversionsprevent coagulation determinations. NOTE: Certain tests may require chilled specimens. Follow recommendedprocedures for Collection and transport. Inversions prevent culture and sensitivity (C&S) urine testing. Minimum urine volume is 5 mL. For lower volumes, submit refrigerated urine in a sterile container urinalysis testing.
4 Inversions ensure preservative is properly mixed. Note the fill lines. Do not under fill (<2 mL) or overfill (>10 mL).SPSC itrate*Citrate ACDGel, serumNo gel, serumHeparinEDTAS odium Flouride(Glucose)FIRSTLASTS topper/Label ColorUrine SpecimensAdditive/Inversions at CollectionLaboratory UseStopperSequenceAdditiveNote: For quality blood sample collecting information, see inside back cover. See Blood, Serum or Plasma in the Specimen Collection and Handling section of this DirectoryQuest DiagnosticsSpecimenCollection TubesLight blue tubesAll plastic Tubes other than light blueClot30-60 minutesSpin10-15 minutesPour intoTransport VialLabel withSpecimen Type Patient IDPatient IDPatient IDSERUMDo not use gel Tubes for toxicology or drug testing* When using a winged blood Collection set for venipuncture and a coagulation (citrate) tube is the first Specimen to be drawn, a discard tube should be drawn first.
5 The discard tube must be used to fill the blood Collection set tubing s dead space with blood but the discard tube does not need to be completely filled. This important step will ensure maintenance of the proper blood-to-additive ratio of the blood Specimen . The discard tube should be a nonadditive or coagulation tube.