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SpectraMax i3x Multi-Mode Detection Platform User Guide

5039228 DDecember2018 SpectraMax i3xMulti-ModeDetectionPlatformWiththeSpe ctraMax MiniMax 300 ImagingCytometerUserGuideSpectraMaxi3xMu lti-ModeDetectionPlatformUserGuide250392 28 DThisdocumentisprovidedtocustomerswhohav epurchasedMolecularDevicesequipment,soft ware,reagents,andconsumablestouseintheop erationofsuchMolecularDevicesequipment,s oftware,reagents, ,inwholeoranypart,isstrictlyprohibited, ,modify,ordistributethesoftwareonanymedi um, ,thelicenseagreementmayprohibitthesoftwa refrombeingdisassembled,reverseengineere d, , productsassuppliedbyMolecularDevicesfori ncorporationintoitsequipmentanddoesnotim plyanyrightand/orlicensetouseorpermitoth erstousesuchmanufacturers ,includingbutnotlimitedto,thefitnessofth isproductforanyparticularpurposeandassum esnoresponsibilityorcontingentliability, includingindirectorconsequentialdamages, foranyusetowhichthepurchasermayputtheequ ipmentdescribedherein, ' , , : , ,SanJose,California,95134, ,LLCisISO9001registered.

SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 2 5039228D ThisdocumentisprovidedtocustomerswhohavepurchasedMolecularDevicesequipment,software,

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Transcription of SpectraMax i3x Multi-Mode Detection Platform User Guide

1 5039228 DDecember2018 SpectraMax i3xMulti-ModeDetectionPlatformWiththeSpe ctraMax MiniMax 300 ImagingCytometerUserGuideSpectraMaxi3xMu lti-ModeDetectionPlatformUserGuide250392 28 DThisdocumentisprovidedtocustomerswhohav epurchasedMolecularDevicesequipment,soft ware,reagents,andconsumablestouseintheop erationofsuchMolecularDevicesequipment,s oftware,reagents, ,inwholeoranypart,isstrictlyprohibited, ,modify,ordistributethesoftwareonanymedi um, ,thelicenseagreementmayprohibitthesoftwa refrombeingdisassembled,reverseengineere d, , productsassuppliedbyMolecularDevicesfori ncorporationintoitsequipmentanddoesnotim plyanyrightand/orlicensetouseorpermitoth erstousesuchmanufacturers ,includingbutnotlimitedto,thefitnessofth isproductforanyparticularpurposeandassum esnoresponsibilityorcontingentliability, includingindirectorconsequentialdamages, foranyusetowhichthepurchasermayputtheequ ipmentdescribedherein, ' , , : , ,SanJose,California,95134, ,LLCisISO9001registered.

2 2018 MolecularDevices, ,Cautions,Notes,andTips5 Chapter1:Introduction11 ComputerIntegration12 Applications12 Optics13 PlateControls13 EnvironmentalControls14 Chapter2:SettingUptheSpectraMaxi3xMulti- ModeDetectionPlatform16 PackageContents16 UnpackingtheInstrument17 RemovingTransportLocks19 ConnectingInstrumentCables22 Chapter3: SpectraMax MiniMax300 ImagingCytometer25 Whole-CellImaging25 SettingUptheCytometer25 Chapter4:GettingStarted42 FrontPanelControlsandIndicators44 LoadingandUnloadingPlates47 TemperatureSettings49 DetectionCartridges50 Chapter5:DetectionCartridges57 AlphaScreenDetectionCartridges58 CisbioHTRFD etectionCartridge61 TimeResolvedFluorescence(TRF-EUSA)Detect ionCartridge63 FluorescenceIntensity(FI)DetectionCartri dges65 FluorescencePolarization(FP)DetectionCar tridges67 GlowLuminescence(LUM)DetectionCartridges 69 DualColorLuminescence(LUM)(BRET2)Detecti onCartridge71 ScanLaterWesternBlot(WB)DetectionCartrid ge74 TransmittedLight(TL)DetectionCartridge77 SpectraMaxi3xMulti-ModeDetectionPlatform UserGuide45039228 DChapter6: SpectraMax InjectorCartridge79 InstalltheInjectorCartridge79 UsingtheInjectorCartridge86 RemovingtheInjectorCartridge105 Chapter7:ReadModesandReadTypes121 SupportedReadTypes122 AbsorbanceReadMode124 FluorescenceIntensityReadMode128 LuminescenceReadMode133 Time-ResolvedFluorescenceReadMode137 FRETReadMode141 HTRFReadMode141 FluorescencePolarizationReadMode144 AlphaScreenReadMode147 ScanLaterWesternBlotTRFReadMode150 ImagingReadMode152 Chapter8.

3 MaintenanceandTroubleshooting155 PreventiveMaintenance155 BeforeYouMovetheInstrument159 PreparingtheCytometerforShipment160 PreparingtheMicroplateReaderforShipment1 67 Troubleshooting174 ObtainingSupport174 AppendixA:InstrumentSpecifications176 MeasurementSpecifications176 CytometerMeasurementSpecifications179 PhysicalSpecifications180 AppendixB:SystemDiagramsandDimensions183 AppendixC:ElectromagneticCompatibility18 7 Glossary1885039228D5 SafetyInformationInformationaboutthesafe useoftheinstrumentfromMolecularDevices includesanunderstandingoftheuser-attenti onstatementsinthisguide,thesafetylabelso ntheinstrument,precautionstofollowbefore youoperatetheinstrument, (SDS) ,cautions, !Iftheinstrumentisusedinamannernotspecif iedbyMolecularDevices, ,Cautions,Notes, ,electrical, ! ! :Atipprovidesusefulinformationorashortcu t, , , (ESD). (WEEE) ,contactyourdealerorlocalMolecularDevice sofficefortheprocedurestofacilitatethepr opercollection,treatment,recovery,recycl ing, ,birthdefects, , !

4 ,maintain, , ,maintaina20 cmto30 cm( in.) ! , i3xMulti-ModeDetectionPlatformisratedaCl ass1 LaserProductbecauseitcanhouseoneormorela sermodules, , ,maintenance, ! MiniMax 300 ImagingCytometerTheSpectraMax MiniMax300 ImagingCytometerhasaLaserClass2 laserinsidetheinstrument, , ,flammable, , ,andtakealltherequiredprecautionswhenusi ngpathological,toxic, ,suchasusingsafetyglassesandwearingprote ctiveclothing, ,flammablesolvents,toxic,pathological, , ! ! , !Iftheinstrumentisusedinamannernotspecif iedbyMolecularDevices, :Nevertrytoexchangelabware,reagents, cm( in.)fortheplatedrawerand15 cm( in.) , ,thereadheightisthegapbetweenthelensandt hetopoftheplate, !Topreventdamagetotheinstrument, , BOTH TRANSPORT LOCKS(CARTRIDGE DRAWER AND PLATE DRAWER)BEFORE SWITCHING THE DEVICE ONTomovetheplatedrawerorthedetectioncart ridgedrawerintooroutoftheinstrument, !Topreventdamagetotheinstalleddetectionc artridgesandtheinstrument, ! :IntroductionTheSpectraMax i3xMulti-ModeDetectionPlatformisamonochr omator-based, :AbsorbanceReadModeonpage124(UVandVisibl e) ,optics, :EndpointReadTypeonpage122 KineticReadTypeonpage122 WellScanReadTypeonpage122 SpectrumReadTypeonpage123 MembraneReadTypeonpage123 Dependingontheapplication,theinstrumentc anread6,12,24,48,96, , !

5 Topreventdamagetotheinstrument,theheight oftheplatemustnotexceed17 mm, ,datadisplay, ,visit: # :Forinformationaboutthecomputerspecifica tionsthatarerequiredtorunthesoftware,the softwareinstallationandlicensinginstruct ions,andthedirectionstocreatethesoftware connectionbetweenthecomputerandtheinstru ment, ,turnoffallsleepandhibernationsettingsfo rtheharddisk,theCPU, ,cellbiology,immunology,molecularbiology , ,nucleicacid,protein,enzymatictypehomoge neousandheterogeneousassays,microbialgro wth,endotoxintesting, , range,PMTG aincanbesettoautomaticoruser-selectedath igh,medium,low, !Topreventdamagetotheinstrument, , ,photomultipliertubevoltageandsensitivit y, !Nevertouchtheopticmirrors,lenses,filter s, , !Useoforganicsolvents(suchasdichlorometh ane) ,makingitpossibletodokineticanalysisofso lid-phase, :Intensity:Low,Medium, :Lengthoftimeinseconds(1to999).SpectraMa xi3xMulti-ModeDetectionPlatformUserGuide 145039228 DYoushouldusetheshakefeatureforELISA sandothersolid-phase, ,andthenmovetheplateoutoftheinstrument, seconds33 seconds68 secondsOptimizedforperformance28 seconds45 seconds96 secondsStop-and-go(off)41 seconds2 minutes<5 minutesPlateReadTimesForOn-The-FlyDetect ion( 5seconds)PlateHeightSensingYoucanuseplat esuptoaheightof25 !

6 Topreventdamagetotheinstrument,theheight oftheplatemustnotexceed17 mm, C( F)aboveambientto45 C(113 F). :Introduction5039228D15 Note:Theinstrumenttemperaturesensorsdete ctthetemperatureoftheairinsidethechamber , , ,nitrogen, , :SettingUptheSpectraMaxi3xMulti-ModeDete ctionPlatformBeforeyouunpackandsetuptheS pectraMax i3xMulti-ModeDetectionPlatform,preparead ry,flatworkareathathassufficientspacefor theinstrument,hostcomputer, :IfyouusetheSpectraMax MiniMax300 ImagingCytometer, , , mmVZ000014 USBcomputerconnectioncable,3 meter( foot)4400-0002or4400-0036 Powercord,115 VorPowercord,230 VForacompletelistofthecontentsofthepacka ge, ! , !Whentransportingtheinstrument, ! , ,placetheinstrumentonadry, ! :IfyouinstalltheSpectraMax MiniMax300 ImagingCytometer, mmhexkeyRequiredToolCAUTION! ! ,suchasharddrives,keycards,andcreditcard s, Detectioncartridgedrawer2 (5) Screw#1fastensthelocktotheinternalframeo ftheinstrument2 Screw#2fastensthelocktotheplatedrawer3 Screw#3fastensthelocktotheplatedrawer4 Platedrawer5 Platechamberdoorinopenposition6 mmhexkeytoloosenscrew# :Afteryouloosenscrew#1, #2and# ,foaminserts,accessoriestoolbox, :IfyouinstalltheSpectraMax MiniMax300 ImagingCytometer, ,3 meter( foot)4400-0002or4400-0036 Powercord,1 meter( foot)ItemDescription1 USBport2 Gasinletquickconnectfitting3 Powerport4 Fusecarrier5 (1) ,nitrogen, ,outsidediameter= mmNotapplicableFlatheadscrewdriverSMS135 100 Gasinletunlocktool(notprovidedwiththeins trument)ItemDescription1 USBport2 Gasinletquickconnectfitting3 Powerport4 Fusecarrier5 PowerswitchWARNING!

7 , :Forthemaximumpermittedairsupplypressure forthegasinletandpolyurethanetubingspeci fications, : SpectraMax MiniMax300 ImagingCytometerYoucanattachtheSpectraMa x ,adigitalcamera,a4xobjectivelens,laserau to-focus, ,flat-bottom, TransmittedLight(TL) (TL) ,orobject-based, ,suchascellsorbeads, ,shape,area, , MiniMax300 ImagingCytometerissufficienttodeterminet heapproximateshapeofsmall8micronobjects, MiniMax300 ImagingCytometerattachestothebottomofthe SpectraMax SpectraMax i3x(microplatereader)2 SpectraMax MiniMax300 ImagingCytometer(cytometer)3 SpectraMaxi3xMulti-ModeDetectionPlatform UserGuide265039228 DNote:IfyouinstalltheSpectraMaxi3xatthes ametimeasthecytometer,unpackthemicroplat ereaderbeforeyousetupthecytometer, :SpectraMaxi3xMulti-ModeDetectionPlatfor mTheSpectraMax ,turnoffallsleepandhibernationsettingsfo rtheharddisk,theCPU, , ,setHarddisk> > > > , , : SpectraMax , , , , , , , , NumberDescriptionSoftMaxProSoftwareLates tversionSoftMaxProSoftwareinstallationgu idewithProductKey5024111 InstallationguideNotapplicableFoampadfro mtheshippingcontainerSpectraMaxi3xMulti- ModeDetectionPlatformUserGuide285039228 DIllustrationPart mmhexkey50249862 Laser-interlockbracketswith4machinescrew s17-100-0010 Flatheadscrewdriver5018965 USBinterconnectioncable, meter(3 foot)50258742 USBcables,2 meter( foot)5022671 SpectraMax i3 PlatformTransmittedLight(TL)DetectionCar tridgeForacompletelistofthecontentsofthe package, : SpectraMax !

8 , !Whentransportingtheinstrument, , ! , ,fourwingnutssecuretheboltsinthecorners, atransportbracketwithsixblueretainingscr ewsandthreemoreblueretainingscrewssecure theopticsplateonthetopofthecytometer, !Nevertouchtheopticmirrors,lenses,filter s, , mmhexkeyChapter3: SpectraMax MiniMax300 ImagingCytometer5039228D31 (A) mmhexkeytoremovethesixblueretainingscrew sthatsecurethetransportbracket(B) mmhexkeytoremovethethreeblueretainingscr ews(C).CAUTION! ,theopticsplatemight"pop" mmhexkeytoremovethethreeblueretainingscr ews(E)thatsecuretheopticscover(D) (topview) ,theopticscover, cm( )wideand65 cm( in.)deep, ( ).WARNING! , mmhexkey50249862 Laserinterlockbracketswith4screws17-100- 0010 ScrewdriverNote:Retainallpackagingmateri als, !Whentransportingtheinstrument, : SpectraMax MiniMax300 ImagingCytometer5039228D33 !IfyouusedtheSpectraMaxi3x, ,RemoveDetectionCartridgesonpage55, ! mmhexkeytoremovethefourscrews(G)thatsecu retheopticsaccesscover(F) (BottomofMicroplateReader)DescriptionF OpticsaccesscoverG (F) (H)overtheopticsaccessopeningwiththeinte rlocktab(I)onthebracketinsertedintotheco rrespondingalignmentslot(J) 2 LaserinterlockbracketsI 2 InterlocktabsJ 2 AlignmentslotsK mmhexkeyandtwoofthebracketscrews(K)tosec urethelaserinterlockbracket(H) (H)withtheinterlocktab(I)insertedintothe correspondingalignmentslot(J),andthenuse theothertwoscrews(K) (I)tomakesurethattheyfreelyslideinandout ofthealignmentslots(J).

9 , : SpectraMax OpticstowerM OpticsaccessopeningN 4 MicroplatereaderfeetO 4 MicroplatereaderfootopeningsWARNING! (L)fitsintotheopticsaccessopening(M)andt hefourfeet(N)onthemicroplatereaderfitint othefourfootopenings(O) ,tools,optics-accesscover,screws, : SpectraMax NumberDescription5018965 USBinterconnectioncable, meter(3 foot),providedwiththecytometer50258742 USBcomputerconnectioncables,2 meter( foot)VN18SS01orVN18FF0101 ACpowercord,providedwiththemicroplaterea derSpectraMaxi3xMulti-ModeDetectionPlatf ormUserGuide385039228 DItemDescription1 MicroplatereaderUSBport2 Gasinletquick-connectfitting3 Powerport4 Fusecarrier5 Powerswitch6 InterconnectUSBport7 CytometerUSBport18 CytometerUSBport29 AlternativepowerinputfortheSpectraMax : SpectraMax MiniMax300 ImagingCytometer5039228D39 ItemDescription1 Powerswitch2 Off3 ,maintaina20 cmto30 cm( in.) (P)suppliedwiththecytometertotheintercon nectUSBportlabeledSpectraMax System(Q)ontherearofthecytometer,andthen connecttheotherendoftheUSBcabletothemicr oplatereaderUSBport(R) USBinterconnectioncable, meter(3 foot),providedwiththecytometerQ InterconnectUSBportR MicroplatereaderUSBportS USBcomputerconnectioncable,2 meter( foot),providedwiththecytometerT CytometerUSBport1U USBcomputerconnectioncable,2 meter( foot),providedwiththecytometerV CytometerUSBport2 Chapter3: SpectraMax meterUSBcable(S) ,andthenconnecttheotherendoftheUSBcablet otheUSBportlabeledCOMPUTERUSB1(T) :Youmustusethe2 meterUSBcable(U) ,andthenconnecttheotherendoftheUSBcablet otheUSBportlabeledCOMPUTERUSB2(V) i3, , !

10 MiniMax300 ImagingCytometer, :GettingStartedNowthatyouinstalledtheSof tMaxProSoftwareonthecomputer,unpackedthe instrument,removedthetransportlocks,andc onnectedthecables, USBport2 Gasinletquickconnectfitting3 Powerport4 Fusecarrier5 PowerswitchToensuresufficientventilation andprovideaccesstodisconnectpowerfromthe instrument,maintaina20 cmto30 cm( in.) , Powerswitch2 Off3 OnTheSpectraMax MiniMax300 ImagingCytometersharesitspowersourcewith theSpectraMax ,usethepowerswitchontherearoftheSpectraM ax ,makesurethatthepowerswitchontherearofth emicroplatereaderisintheOff Touchscreen2 Detectioncartridgedrawer3 Platedrawer4 Statusindicatorsonthetouchscreen5 CytometerstatusLEDsThefrontpanelontheSpe ctraMax ,accessthetemperaturecontrols, ,orange,yellow,green, , ,drawers, IndicatorCytometer :GettingStarted5039228D47 , Touchscreen2 Platedrawer3 WellA1ontheplate4 !Topreventdamagetotheinstrument, , ,theinstrumentcanread6,12,24,48,96, , nm,youmustusespecialUV-transparent,dispo sable, :FortheSpectraMaxi3x, 'sspecificationsforwellsize,spacing, !