Spectrophotometers Models 7310 & 7315 - Jenway

1 SpectrophotometersModels 7310 & 7315 Operating Manual731 005 REV E/12-1023 SafetyPlease read this information carefully prior to installing or using this The unit described in this manual is designed be operated only by trained personnel. Any adjustments, maintenance and repair must be carried out as defined in this manual , by a person qualified to be aware of the hazards It is essential that both operating and service personnel employ a safe system of work, in addition to the detailed instructions specified in this Other than for those items defined in the maintenance procedures herein there are no user serviceable items in this instrument. Removal of covers and attempted adjustment or service by unqualified personnel will invalidate the warranty and may incur additional charges for References should always be made to the Health and Safety data supplied with any chemicals used.

2 Generally accepted laboratory procedures for safe handling of chemicals should be If it is suspected that safety protection has been impaired in any way, the unit must be made inoperative and secured against any intended operation. The fault condition should immediately be reported to the appropriate servicing Contents PageSafety 3 SeCtion 1 - introduction Instrument description Instrument specification 8 SeCtion 2 - installation Unpacking Installation Display Controls Rear panel Front panel 15 SeCtion 3 - theory and practice of spectroscopy measurements Theory of spectroscopy measurement Spectroscopy measurement Good practice guidelines 17 SeCtion 4 - instrument setup Navigating and screen setup Time and date Instrument settings menu Security and setting passwords Setting security codes Settings lock Method lock Mode selection Diagnostics GLP settings Screen contrast Lamp save 23 SeCtion 5 - Photometrics Mode specific parameters

3 Method set up Selecting a wavelength Calibration Sample measurment 26 SeCtion 6 - Concentration Mode specific parameters Method setup Selecting a wavelength Settings Selecting concentration units Changing the resolution Using a standard Using a factor Calibration Calibrating to a standard Calibrating to a factor Sample measurement Measuring a sample after calibrating to a standard Measuring a sample after calibrating to a factor 30 SeCtion 7 - Spectrum Mode specific parameters Method setup Scan settings Selecting absorbance or % transmittance Setting start and end wavelengths Setting the scan interval Y-axis scaling Calibration Sample measurement Data analysis Peaks and valleys threshold Peaks and valleys table Spectral points analysis 36 SeCtion 8 - Quantitation Mode specific parameters Method setup Selecting a wavelength Quantitation table Selecting number of standards Selecting concentration units Changing the resolution Selecting absorbance or % transmittance Adding standards Standard curve Creating a new standard curve Calibration Sample measurement Data analysis 43 SeCtion 9 - Kinetics Mode specific parameters Method set up Kinetics settings Y-axis scaling Setting lag time or start on level Selecting absorbance or % transmittance Changing the resolution Selecting concentration units Using a standard Using a factor Selecting a wavelength Setting the kinetics measurement time Calibration Sample measurement Data

4 Analysis 49 SeCtion 10 - Saving, printing and autologging Saving methods Saving methods to internal memory Saving methods to USB memory stick Opening methods Opening methods from internal memory Opening methods from USB memory stick Deleting methods Saving results Opening results Deleting results Printing Print setup Print setup photometrics and concentration Print setup - spectrum Print setup quantitation Print setup kinetics Printing results Autologging Setting the number of sample repetitions Selecting result s destination Connecting to a PC 59 SeCtion 11 - Accessories and spare parts Optional accessories Connecting the accessories Internal printer Passive accessories Active accessories Automatic 8 cell turret Peltier Sipper pump

5 Combined sipper peltier pump Using the accessories Automatic 8 cell turret Supporting creation of a standard curve in quantitation Peltier Sipper pump manual sipper pump settings Timed sipper pump settings Combined sipper peltier pump Spares 70 SeCtion 12 - Maintenance and service Routine maintenance Lamp replacement Tungsten halogen lamp replacement Xenon lamp module replacement Service 72 SeCtion 13 - troubleshooting Error codes Troubleshooting guide Technical support 75 SeCtion 14 - Declaration of conformity 76 SeCtion 15 - Glossary of icons 78index 85910 SeCtion 1 - introduction inStRUMent DeSCRiPtion The 7310 and 7315 Spectrophotometers are suited to a wide range of applications in education, quality control, environmental and clinical analysis.

6 The 7310 is a visible spectrophotometer covering a wavelength range from 320nm to 1000nm. The 7315 is a UV/Visible spectrophotometer with a wavelength range from 198nm to 1000nm. Both Models have five measurement modes: photometrics, concentration, spectrum scanning, quantitation and kinetics. These instruments use icon driven software and have an improved navigation system for easy and intuitive usability. inStRUMent SPeCiFiCAtion 7310 7315 WavelengthRange 320 to 1000nm 198 to 1000nmResolution 1nmAccuracy 2nmRepeatability bandwidth 5nmPhotometricsTransmittance 0 to to 1%T, at AbsorbanceResolution , light < at 340nm < at 340nm and 220nmConcentrationRange -300 to 9999 Resolution Selectable 1 Blank with a single standard or factorUnits no units, %, ppm, EBC, SRM, mEq/l, mEq, M, mM, M, nM, U, U/l, U/ml, g/l, mg/l, g/l, ng/l, g/dl, mg/dl, g/dl, mg/ml, g/ml, ng/ml, g/ l, ng/ l, mol/l.

7 Mmol/lFactor to 10000 Standard to 1000 QuantitationRange -300 to 9999 Resolution Selectable 1 Blank with up to 6 standardsUnits no units, %, ppm, EBC, SRM, mEq/l, mEq, M, mM, M, nM, U, U/l, U/ml, g/l, mg/l, g/l, ng/l, g/dl, mg/dl, g/dl, mg/ml, g/ml, ng/ml, g/ l, ng/ l, mol/l, mmol/lCurve fit algorithms Quadratic, quadratic through zero, linear, linear through zero, interpolateKineticsMeasurement Time 2 to 9999 secondsCalibration Blank with a single standard or factorDisplay Concentration, rate of change, initial and final absorbance/%TResolution Selectable 1 7310 7315 SpectrumRange 320 to 1000nm 198 to 1000nmScan interval Selectable 1, 2 or 5nmAnalysis Absorbance or % transmittance and peak and valley wavelengthsotherBeam height 15mmLight source Tungsten halogen lamp Xenon lampLamp save Yes Not applicableGLP Current time and date, user ID, settings lock and method lockNumber of users 999 Methods memory 48 in each measurement modeResults memory Limited by attached mass storage deviceRemovable media USB (supplied)Outputs USB, Analogue, RS232, Internal printerPower 24 VSize (w x d x h) 275 x 400 x 220mmWeight 6kg12 SeCtion 2 - installation UnPACKinG Remove the 7310 or 7315 from the packaging and ensure the following items are included: 1.

8 Model 7310 spectrophotometer (731 001), or Model 7315 spectrophotometer (731 501) 2. 24V 65W power supply unit (021 060) 3. Pack of 100 disposable plastic visible wavelength cuvettes (060 084), or pack of 100 disposable UV plastic cuvettes (060 230) 4. 2 GB USB memory stick (019 146) 5. Jenway 73 series PC software (735 100) and interface cable (013 203) 6. Instruction manual (731 005) 7. Jenway Foreign manual CD (JENMANCD) 8. Optional accessories (as ordered) inStALLA tion Models 7310 and 7315 are supplied ready to use. The unit should be placed on a clean flat surface which is free from drafts and vibrations. The units are designed for operation on 90V to 264V AC input at 47 to 63Hz. Select the correct plug attachment and attach to the power supply unit as shown below: Fig Power supply unit with various plugs Connect the power supply unit to the power inlet socket on the rear panel of the instrument and connect to the mains socket.

9 Turn the power on at the mains and switch the instrument on using the power switch on the rear of the instrument. The instrument will perform several power on tests before displaying the main menu: 13 Fig All Power On Tests Complete1. Instrument check ensures the validity of the saved parameters2. Dark test3. Checks for the accessory fitted. If an active accessory is found the instrument verifies communication and response4. Self calibration of wavelengths5. Checks communication between USB memory stick port and the DiSPLAYT hese Spectrophotometers have a dot matrix display which enables icons and graphs to be displayed clearly. Following successful completion of the power on tests the main menu screen will be displayed:Fig. Display1. Spectrum measurement mode2. Photometrics measurement mode3.

10 Back key4. Time and date menu5. Quantitation measurement mode6. Instrument settings menu7. Concentration measurement mode8. Kinetics measurement mode 731009:02731009:022134123874556 ContRoLSThe keypad used for these Models enables an easy and effective way of navigating the different measurement modes, entering numbers, saving and analysing results. The soft keys are active when an icon is displayed above or adjacent to the key. The only exception to this is the back key which is always active. The main menu screen and surrounding keypad is displayed below. Fig. Display1. Spectrum measurement mode2. Photometrics measurement mode 3. Back key4. Time and date menu5. Quantitation measurement mode6. Instrument settings menu7. Concentration measurement mode8. Kinetics measurement mode 731009 ReAR PAneLThe image below shows the rear panel on the instrument:Fig.