stabilised vitamin C from Hayashibara

1 stabilised vitamin C from Hayashibara for healthier, brighter, younger looking skin AA2G (Ascorbic Acid 2-Glucoside) from Hayashibara is natural vitamin C (ascorbic acid) for brighter skin stabilised with glucose. This combination allows the bene ts of vitamin C to be conveniently AA2G can function in essentially an identical and effectively used in cosmetic products. manner to vitamin C, preventing pigmentation When creams and lotions containing AA2G of skin by suppressing melanin synthesis in are applied to the skin, an enzyme present in melanocytes. It also has the ability to reduce the skin, -glucosidase, acts on the AA2G to the amount of pre-existing melanin, resulting in a slowly release the healthful bene ts of vitamin C. lighter pigmentation of the skin. AA2G was originally developed as a quasi-drug cosmetic product in Japan to lighten the overall Without AA2G.

2 Tone of the skin and reduce the pigmentation in age spots and freckles. Further research has melanin shown other dramatic bene ts and today AA2G . is used all over the world not only for whitening but also for brightening dull looking skin, reversing the effects of aging, and in sunscreen products for protection. High stability (x 200). AA2G has glucose . bound to the hydroxyl With AA2G ( ). group of the second carbon (C2) of the ascorbic acid. The C2 hydroxyl group is the primary site of natural vitamin C's bene cial activity; however, this is the site where vitamin C is degraded. The glucose protects vitamin C from high temperatures, pH, metal ions and other mechanisms of degradation. (x 200). Sustainable vitamin C activity The ef cacy of AA2G was tested using B16 melanoma cells treated for 12 hr with AA2G ( ) or a placebo.

3 Following When products containing AA2G are used on treatment theophylline ( ) was added to stimulate melanin the skin, the action of -glucosidase gradually synthesis, and the cells were tested after 48 hr for the presence of releases vitamin C, providing the bene ts of DOPA quinone (a precursor to melanin) using a histochemical stain. This shows that AA2G can prevent unwanted skin pigmentation. vitamin C effectively over a prolonged period [Yamamoto et al., Nishinihonhifuka, 58 (3), 439-443 (1996)]. of time. Formulation bene ts AA2G is more soluble than natural vitamin C. for healthy skin It is stable over a wide range of pH condition, AA2G slowly releases vitamin C, which has especially at pH which is typically been shown to promote the synthesis of collagen used for formulation of skin care products.

4 By human skin broblasts, thereby increasing AA2G has been shown to be easier to the suppleness of the skin. AA2G can provide formulate than other vitamin C preparations. these bene ts over a prolonged time period. AA2G . AsA. Collagen/total protein %. control DAYS DAYS DAYS. Human skin broblasts were incubated in the presence of a control, ascorbic acid (AsA; ) or AA2G ( ) for 1- 5 days. The percentage of collagen synthesis to total protein synthesis was determined. This shows that AA2G can promote collagen synthesis. Stimulatory effect of AA2G . continued for the full 5 day period. [Yamamoto et al., , 122, 871-877 (1992)]. for sunlight protection Oxygen radicals, which are formed in the skin during its exposure to sunlight, are believed to cause skin damage and reddening of the skin.

5 AA2G slowly releases vitamin C, which acts as a free radical scavenger, thereby reducing the amount of skin in ammation and roughness. number of cases, 9 days after UV radiation effective partly effective ineffective partly reverse reverse 0 1 2 3 4 5 6 7 8 9 10. In a controlled test, creams containing 2% AA2G or placebo were applied at two places on the inside of the right arm of 15 healthy adults. The creams were applied three times a day for 6 consecutive days. During this treatment, the arms were irradiated with UVA and UVB once per day for a total of 3 days. This shows that AA2G can prevent UV skin damage as measured by a reduction in skin erythema. [Miyai and Yamamoto et al., Nishinihon , 58 (3), 439 (1996)]. for younger looking skin AA2G slowly releases vitamin C which acts to reduce ne lines and wrinkles, resulting in younger looking skin.

6 6 200. Wrinkle height ( M). Roughness 5 150. 4. 0 45 90 100. 0 45 90. DAYS DAYS DAYS DAYS DAYS DAYS. Sixteen female volunteers ranging in age from 37 to 55 years, Sixteen female volunteers ranging in age from 37 to 55 years, applied a face cream containing 2% AA2G . The change in mean applied a face cream containing 2% AA2G . The change in mean roughness pro les was determined using silicone casts at onset depth of the wrinkle pro les was determined using silicone casts at and after 45 and 90 days. The results showed that there was a onset and after 45 and 90 days. The results showed that there was signi cant difference ( p < ) in reducing the roughness of the a signi cant difference ( p < ) in reducing the average depth of skin after 45 days of use and these bene cial effects continued with wrinkles after 45 days of use and these bene cial effects continued further use.

7 With further use. INCI name: Ascorbyl Glucoside AA2G is manufactured by Hayashibara Company, Ltd. Okayama Japan Hayashibara International Inc. 390 Interlocken Crescent Suite 680. Broom eld Colorado 80021. USA. Tel: 303-650-4590. Fax: 303-650-9860. Hayashibara International UK. Global House 1 Ashley Avenue Epsom KT18 5AD. United Kingdom Tel: +44 (0) 1372 822 980. Fax: +44 (0) 208 823 9001. Email: International of ces: Bangkok Denver Dubai D sseldorf London Moscow Mumbai S o Paulo Shanghai Sydney