히알루론산 하이드로겔의 가교도에 따른 함수특성 Swelling …

1 Biomaterials Research (2013) 17(1):37-4037 BiomaterialsResearchCThe Korean Society for Biomaterials Swelling Properties for Cross-linking Degree of Hyaluronic Acid Hydrogels 1 2 3 4 4 5 1*Jin Tae Kim1, Deuk Yong Lee2, Yoo Seoung Oh3, Jung Wan Bang4,Chang-Yong Hyun4, Tae-Hyung Kim5, and Jae Ha Choi1*1 , 2 , 3 ,4 , 5 1 Department of Advanced Material Engineering, Chungbuk National University.

2 Cheongju 361-763, Korea2 Department of Materials Engineering, Daelim University, Anyang 431-715, Korea3 Department of Prosthetics and Orthotics, Chungbuk Health & Science University, CheongWon 363-794, Korea4 Department of Advanced Material Engineering, Seoul National University, Seoul 139-743, Korea5 Department of Radiological Science, Kangwon National University, Samcheok 245-905, Korea(Received November 02, 2012/Acccepted January 30, 2013)Hyaluronic acid (HA) hydrogels were crosslinked by dropping of sodium hyaluronate solutions into a solution mixtureof divinyl sulfone (DVS) dissolved in 2-methyl-1-propanol.

3 Then cleaning for residual unreacted DVS, and drying at60oC, 20torr vacuum. The experimental conditions are crosslinking temperature (CLT), crosslinking agent concentration(CAC), respectively. And then parametric studied the morphology, the porosity, the Swelling ratio of the hydrogels. Theswelling ratio and pore size were decreased as the degree of crosslink was increased. They can be controlled by adjust-ing the by varying CLT and words: hyaluronic acid, hydrogel , Swelling properties, porous network , , Van der Waals.

4 1,2) 3 , , .3-6) , , , .. , .. * : Figure 1. Chemical structure of cross linked hyaluronic acid by divinylsulfone. (a) HA, (b) DVS, (c) cross linked HA by Biomaterials Research 2013.

5 7,8) OH pH (Figure1) . (Streptococcus, Lancefield C type, Mw =1 106Da, Shiseido Co., Japan) mol/L NaOH 24 wt% . 3,6,9) pH 11-12 (2-methyl-1-propanol, 99% Junsei, japan) vol% ( 97%, Aldrich, USA) mL/min 21G.

6 5psi (Figure 2).6) 5 , 200~300 rpm . Table 1 . , 30 3 60oC, 20 mmHg 24 . 24 (pore size m, Advantec, Japan) .. Swelling % = 100[%]Ws : Wd : SEM(S-3000H, Hitachi, Japan) (SV-55, SOMETECH, Korea).

7 6) 80~120 m (Figure 3) Figure 4 .CLT 6) . 10oC 2 , Arrhenius equation 10) , 40oC, 50oC, 60oC . CAC , , , vol% , , vol% DVS, 5 , vol%DVS, 25.

8 WsWd Wd------------Figure 2. Schematic representation for the preparation system of HAmicrobeads. (a) Dispenser for HA solution delivery, (b) Compressed airpump system, (c) Spray nozzle, and (d) Cross-linking bath and stirrer.[9]Table 1. Experimental conditions of parametric studies for hydrogelsclassificationcrosslinking injection air pressure (psi)DVSconcentration(vol%)time (h)temperature (oC)CLT5A B C 40 50 60 CAC5RT5A B C 39 Vol. 17, No. 1 CLT 60oC (Figure 3) 6) CAC Figures 5.

9 CLT CAC (Figure 6).. , . 6,11,12) . , Figure 3. SEM image of the surface of HA hydrogels (a) 60oC, vol% DVS of CLT group, (b) room temperature, vol% DVS of CAC group, (c)room temperature, vol% DVS and 25hr crosslink of control 4.

10 Stereoscopic microscope image of hydrogels by rehydratedmicro 5. SEM image of the porous network of HA hydrogels (a) 60oC, vol% DVS, of CLT group, (b) room temperature, vol% DVS of con-trol group, (c) room temperature, vol% DVS of CAC 6. Swelling ratio of HA hydrogels as functions of CLT, CACrespectively. Note that the parameters were varied in 4 steps (A, B, C,D), respectively, as listed in Table Biomaterials Research 2013 .12-1).