Swine Health Test Swine Health Laboratory Use Only Test ...

1 Laboratory Use Only Case No. Swine Health Test request Test FormForm request No. Samples College of Veterinary Medicine Page Page 1 1. Veterinary Diagnostic Laboratory 1600 South 16th St. | Ames, IA 50011-1250. 515-294-1950 | Fax 515-294-3564 | VETERINARIAN_____ Test Selection & Sample Type Identification on Page 2 >. Clinic_____. ANIMAL LOCATION: Premises, Herd and Submission-Level Identifiers Address_____. City, State & Zip_____ SITE NAME_____. Phone_____ Fax_____ Address_____. Email_____ City, State & Zip_____. Accreditation # (if regulatory)_____ County_____ Country_____. If Owner Name and Address are same as Animal Location (include info under Site Name) Premises ID# (attach premises ID bar code sticker if available). OWNER_____. Division_____. Address_____. City, State, & Zip_____. Lot or Group ID_____ Premises Type Third-Party Billing (pre-approved) (Best Description).

2 Source or Flow ID_____. _____. Reference (Other)_____. Boar Stud Affiliates (list codes) Breeding Herd _____. Vaccine Usage Date Collection Point (Slaughter/Market). Vaccine Name Given Dose Exhibition Center Special Reporting Requests Farrow to Feeder/Finish Phone_____ Grow-Finish (or Wean to Finish). Fax_____ Isolation or Growing Replacement Stock Email_____ Non-Commercial Livestock x Porcine Species: Reason for Test Nursery General Diagnostics Truckwash SAMPLES. Other_____ University or Research Center Collection Date_____ No. of Samples_____ (Specify reason for testing if for official regulatory purposes) Other_____. Consecutively numbering samples ( 1, 2, 3, 4, ) greatly enhances receiving, accessioning, and sample processing efficiencies within the Laboratory . Age (check unit) Age (check unit). Sample d wk mo Location Parity Sample d wk mo Location Parity ID # Animal ID yr adult (Other) Gender (#) ID # Animal ID yr adult (Other) Gender (#).

3 1 16. 2 17. 3 18. 4 19. 5 20. 6 21. 7 22. 8 23. 9 24. 10 25. 11 26. 12 27. 13 28. 14 29. 15 30. The ISU VDL is a fully accredited Laboratory by the American Association of Veterinary Laboratory Diagnosticians and a member of the National Animal Health Laboratory Network. A complete description of ISU VDL's diagnostic services, submission guidelines, client confidentiality policy, and the contractual terms associated with the requests and performance of diagnostic services at the ISU VDL are available at the ISU VDL web-site ( ). Diagnostic specimens submitted for serological or molecular diagnostic testing are generally retained for 2 (serology). to 4 (molecular) weeks from the date received should the need for additional testing arise. Document ID Page 1. Laboratory Use Only Case No. Swine Health Test request form No. Samples College of Veterinary Medicine Page 2.

4 VETERINARIAN_____ SITE NAME_____. SAMPLE TYPE Serum Oral Blood Nasal Feces or Environmental Other Fluids Swab Swab Fecal Swab _____. CONSECUTIVE SAMPLE ID#'S _____ to_____ _____ to_____ _____ to_____ _____ to_____ _____ to_____ _____ to_____ _____ to_____. Expected PRRSV Status Expected _____Status Export to:_____. HATS Submission Pos Neg Pos Neg Ship date:_____. x PRRSV 1 - 10, . All samples will be tested for each assay requested unless noted in the column Test Samples ( , x PEDV 11 - 20). SEROLOGY See ISU VDL website: for complete listing of tests , fees, and submission guidelines. Test Test Test Test Test Samples Samples Samples Samples Only Samples APP CF (1-5-7) _____ Erysip. (Lumx) _____ PEDV FFN _____ H1 gamma HI _____ VS VN _____. APP CF sero (3) _____ Hepat. E ELISA _____ PEDV IFA Screen _____ H1 Delta 1 HI _____ _____ _____.

5 APP CF sero (1) _____ Lawsonia ELISA _____ PEDV IFA (4 Dil) _____ H1 Delta 2 HI _____ _____ _____. APP CF sero (5) _____ Lepto (5) MAT _____ PRRS X3 ELISA _____ H1 pandem. HI _____ _____ _____. APP CF sero (7) _____ Lepto (6) MAT _____ PRRS ELISA OF _____ H1 Classical HI _____ _____ _____. APP Mix ELISA _____ M hyo IDEXX _____ PRRS IFA NA scr _____ H3 Cluster 4 HI _____ _____ _____. APP 10-12 ELISA _____ M hyo Dako _____ PRRS IFA EU scr _____ H3 Cluster 1/3 HI _____ _____ _____. Brucella BAPA _____ PPV HI _____ PRRS IFA NA end _____ Zoetis Ags HI _____ _____ _____. Brucella Card _____ PCV2 ELISA _____ PRRS IFA EU end _____ Harris Ags HI _____ _____ _____. Brucella FPA _____ PCV2 IFA (4 Dil) _____ PRRS FFN _____ Homologous HI _____ _____ _____. Brucella STT _____ PCV2 IFA end pt _____ PRV gB ELISA _____ TGE/PRCV ELISA _____ _____ _____.

6 Brucella SPT _____ PCV2 FFN _____ PRV VN _____ TGE VN _____ _____ _____. Brucella RAP _____ PEDV ELISA _____ SIV NP ELISA _____ Toxoplasma ELISA _____ _____ _____. MOLECULAR & VIRAL DIAGNOSTICS Samples tested individually, unless otherwise indicated. SEQUENCING. See ISU VDL website: Pool All Samples in Groups of _____( 5) for guidance on appropriate sample types. PCV2 _____. Test Positive Test Positive PCR. Test Test Pool Pools PCR. Test Test Pool Pools PEDV _____. Samples Individually ( < or=5) Individually Samples Individually ( < or=5) Individually PRRS (ORF5) _____. A suis _____ ____ PEDV differential _____ ____ SIV (HA) _____. APP _____ ____ PEDV quantitation _____ ____ VIRUS ISOLATION. HPS _____ ____ PPV _____ ____ . Lawsonia _____ ____ PRRS _____ ____ PCV2 SIV. Lepto _____ ____ PRRS quantitation _____ ____ PPV _____. M hyopneum _____ ____ Rota (A,B,C) _____ ____ PRRSV _____.

7 M hyorhinis _____ ____ SIV screen _____ ____ Forward Isolate to _____. M hyosynoviae _____ ____ SIV + Subtype _____ ____ Special Instructions for Sequencing & Virus Isolation M suis _____ ____ SIV (USDA Surv) _____ ____ ( # per case, group, location). PCV2 _____ ____ TGE _____ ____ . PCV2 quantitation _____ ____ _____ _____ ____ . PDCoV _____ ____ _____ _____ ____ . PEDV _____ ____ _____ _____ ____ . BACTERIAL CULTURE Culture/ID Sensitivity Save Isolate *Please include Age with sample-level information and note Syndrome below to aid in culture setup. Forward Isolate to _____ Test Samples _____ Check all that apply: Specific organisms/special test _____ Enteric Respiratory Systemic Additional Information or Test Requests: The ISU VDL is a fully accredited Laboratory by the American Association of Veterinary Laboratory Diagnosticians and a member of the National Animal Health Laboratory Network.

8 A complete description of ISU VDL's diagnostic services, submission guidelines, client confidentiality policy, and the contractual terms associated with the requests and performance of diagnostic services at the ISU VDL are available at the ISU VDL web-site ( ). Diagnostic specimens submitted for serological or molecular diagnostic testing are generally retained for 2 (serology). to 4 (molecular) weeks from the date received should the need for additional testing arise. Document ID Page 2.