Transcription of 気管支肺胞洗浄液 ... - sysmex-labscience.jp
1 090021/2 Bronchoalveolar Lavage Fluid BALF 1978 BAL BALF BALF BALF 50 g OA 1mg 12 2 22 2 10 1% OA (UN-701 : 3ml/min : 4-5mm ) 30 4 3 22 26 30 24 BALF Th2 IgE BALF BALF pentobarbital sodium (60mg/kg, ) 3 Fr BSA mM EDTA-2Na Ca2+, Mg2+ PBS ( PBS )
2 Ml 4 3 BALF 2009 6 09002 Bronchoalveolar Lavage Fluid 2/2 PBS 1 ml BALF PBS BALF BALF PBS BALF PBS(-) Ca/Mg EDTA BSA 651-2271 4 4 4 Tel.
3 (078) 991-2091 Fax (078) 997-9976 URL by sysmex CORPORATION SCIENTIFIC INSTRUMENTATION BUSSINESS DIV Copyright @ 2009 by sysmex CORPORATIONNo part of this publication may be reproduced without the prior the written permission of the publisher. Printed in Japan. Cytospin II (Shandon) 300 rpm 4 200 l PBS Diff Quick ( ) 300 BALF BALF -80 C BALF enzyme linked immunosorbent assay (ELISA) (IL-4 IL-5 IFN- : ENDOGEN.)
4 IL-13 TGF-b1 : R&D systems) BALF BALF BALF BALF 4 C 800 g 10 -80 C ml PBS 50 l 150 l Turk ( ) BALF Burker 0123456 BALB/c 0123456C57BL/6 OA Saline OA Saline To talMacNeuEosLymTotalMacNeuEosLymB A LF (x 105)(x 105)**NDND** 24 BALF
