Task Force Committee for Evaluation of Anti-aging Function

1 Guideline for Evaluation of anti -wrinkle ProductsTaskForce Committee forEvaluationof Anti-aging Function 1. # ( ( anti -wrinkle products) #01 # EU, ( 0 1 # 6 8 9 9 = @ B C # # EU # COLIPA (The European Cosmetic,Toiletry and Perfumery Association) !F # J K M N # $PQ% &' R) +8 T B N V X N [\ ]^ _- Q% [ `a F0 EU 1994/ b = @(EEMCO: European Group for theE$cacy Measurements on Cosmetics and Other TopicalProducts) d 3 C ef g F# 45 ij # ^ 7 #81) 9:;2) 9:<f= 3) 9 pH4), TEWL5) 3 lm no= 9: >M?]

2 36) # @r # 6A BC # E 0 T # tFGu # C # # R! vwH x I R!8 0 ?y 7 z9 {# T L M # 9: O P | }u BC # Q~ R B 6 BC # C V& ^ EUXY ef Z[ # 3 m BC z ^ A3 ^ ` a BC [c d 10/ 3 e 5/f g ] 3 C h z 3 cO 3 j F# ^ f ! 7) 11) ^ P ? # 0 7 tFGu C no 8 F 0 3 ` s h th 12) lm 3 E u v w ` x g ay ay C # 12) | # 3 ay T wH ~ a E A3 # # T m X # V& T 2.]

3 2 1. # ay G u ay 1 3 Fig. 1 ` Q v e / # 0 F0 X6 G F0L X T u v G 0 G e L ^ m j ] C BC f 169 0075 1 L4 4 19 Secretariat O$ce of Japanese Cosmetic Science Society(4 4 19 Takadanobaba, Shinjuku-ku, Tokyo 169 0075, Japan)BC f Vol. 30, No. 4, pp. 316 332 (2006) 1) 2) 3) 4) ! % & ' 5) ) &* % - 2 2. 1 2 3 4 5 6 7 8) !;< >" ?- # - BC & %F H ' ( 7 8) >"B*+ ?

4 - 5 ?- P 8) 5 . 0 R TU III, IV Fitzpatrick 12 - 3 5V W XY 8Z 5V [ ]9 ^ _ ` 4 a= = ?@%F ?- bc 5 DE 5 5 5 def def g% ?- g h B 2 3. jd#4 d jd ! %IJ%R 5 Q Uo Rr%F % PMN Ot PQN v wQ%F x %y S T V ?- C [ 9 {| ?- % }Y C R [ R [ V [ jd \_ o ] ^B [ &_ [ 2 4. C Ia b Q [ %F c C jd [ P #P & B jd f P C % C gh g kl n P%F p B 2 5. rB 1%jd-B 2 d jd % !;% - 2 6.]]]]]]]

5 Uv w B z% E B ; { %| g ?- E 2 7. 2 7 1. C &{|# \_ 3 ^ C &{|# \_ 8Z %o 7 o [ 7 gh g # U 5 # 3 2 11. 0 ) gh% 3 5 2 7 2. bc a= = ?@ _ ` v v [B jd ` % a= 20 22 = 50 5 B B U w ! V jd \_ 151dbc w {| U _ \_- 2 7 3. " ^ U 2U ? ?- 2 7 U 2U ? ?- 2 7 5. 2 7 5 1. 3 5V & &-\w ' & ? &* 3 4 Trained Expert o 5V B &# o 5V 5V 4 n {| o (Fig.]]}}}}}

6 1) {| R ' 3 - 4 }B 3 o S } 9 o 5V U 2U 317 . Vol. 30 No. 4 (2006)318 (Fig. 1) ! , . / 0 , 1/4 12 5 2 7 5 2. : ; 0 , : ; < :; ;? @ A Train-ed Expert B CD (Fig. 1) F H , . / 0 , 1/4 12 5 2 7 5 3. I K L ! ", #MNO P & I K QMNP & in vivo ' & QMNP & ,R )& ", F H T P W Y 2 8. Z" [ 7Z" ]F H ^ _- `. a b c 2 9. de f/ 0 h i ,e 0 I mnop 3 ,1 ,r 4s" I Z"t 5u i ,e 0 I mnop F, 27 0 mnop 8 ,1 ,r ,R e o 1 5uF H<v w ; > ?

7 > @AF HBt Dt <v xE oF ;? I mnop y, G 2 10. ,R B H F, 1 JTP K& ", { N| I N OP Q} ? N| . R~ P 0 A T , Z" V Z"& X 0 f/ X27 de 0 , 4s" . ["\{ Z" ^ x H , | F, ^ P I Z" P r 2 11. ! #$ ' ^ P n { N| F, ? N| R~ H B b(p ) -` 0 , ? N| R~ P W Q} (p ) -` ^ 0 G 2 12.( c 15e7 30f CD o g h c 15e ijk 255 g n n o- . q ! s u v x z{ z {@Q@ ~ 3. - /0 0 6 3 H . H 3 5 Fig.)]}}}

8 1 ^ { N| I N| |/ N N e N ! , r?s r x r 0 . ?s ^ { N 1r I N |/ R . 5 R Ax . 1)} 0 2) D& , 3) 4). F5 r A;D < 0 5). oF ;? , -` H A r H Q ^7 u ` B 3193 2. "# # &' ) +,- / & 3 "# 5 "# 8 5 : < III, IV Fitzpatrick # ? @ A CD E ! @ F G I" JK M! N O (),- "# PQ 5 - 5 5 5 UV !}

9 UV 0X, "# X \1& 3 3. ^U 42U ^U + K` 4 ,56,: b5 8 f ) :i,- 9 , 8 ; k =l; m n ,- o?,p AB C "# ' G E" rs "# , tF ' : G Gv : bGv wH C wH ^U MGv fx y z & Hv {LM |OwH 3 4. ' ) Gv 5 }Q ~ 8 G R ,- T ' ) ^U ! H V != = & ^U ! != ' ) , ' ) YZ X [\ G_` G_=,- ~a b & 3 5. d& ,^U#& 2 f ^U ! , , # 3 6. gh Q & , & , oX "# ! 3 7. 3 7 1. ' ) rs Vq M + ? JK ' ) rs Vq M + E ! st ,fx v fxF G v YZ X Vq 5 ~ 3 y!]

10 YZ, 5 3 7 2. !PQ O () M! N h m wH& ^U N z , O 20 22 50 5 { & & | b K` C ^U M ~ 15 UPQ 3 ! ! + rs ! M! ! M# 3 7 3. " JK ( "# 3 7 ! ( "# 3 7 5. 3 7 5 1. ? @ { {# w { " { Trained Expert fx @ & fx F @ @ ` rs fx F (Fig. 1) rs : b ! ! rs ! (&, fx F (Fig. 1) : b F , U 1/4 : b F| # 8 ,- 3 7 5 2. " { {# w { " { Train-ed Expert fx @ & fx F (Fig.))))}}}}}}}}}