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TCID 50 protocol - University of Rochester
2. Calculate 50 % end point. Log lower dilution= dilution in which position is next above 50% Example above: Log lower= 10-6 or -6 3. Add PD and Log lower dilution Example above: -6 + .375 =-6.375 Log TCID50= 10 – 6.375 or 1/ 2.37 x10 6 4. Calculate TCID 50/ml. Divide by the ml of viral innoculum added to row A
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