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学位論文の内容の要旨 - tmd.ac.jp

Intraarticular injection of synovial stem cells promotes meniscal regeneration in a rabbit massive meniscal defect model MSC MSC PBS 107 MSC PBS 2 DiI MSC 4 12 16 24 ( n=4) 24 (n=4) 2 MSC 4 12 MSC 16 24 MSC 24 MSC MSC MSC MSC 70 MSC MSC - 1 - MSC MSC MSC

個の滑膜mscを、対側には同量のpbsを注射した。 投与2週後に、dii標識した滑膜mscの局在を観察した。4,12,16,24週後、肉眼的、組織学的

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Transcription of 学位論文の内容の要旨 - tmd.ac.jp

1 Intraarticular injection of synovial stem cells promotes meniscal regeneration in a rabbit massive meniscal defect model MSC MSC PBS 107 MSC PBS 2 DiI MSC 4 12 16 24 ( n=4) 24 (n=4) 2 MSC 4 12 MSC 16 24 MSC 24 MSC MSC MSC MSC 70 MSC MSC - 1 - MSC MSC MSC

2 5 104 cells/mL 7 passage 0 3 103 cells /mL 5 passage 1 passage 1 60 cm2 100 14 105 21 21 -O 21 MSC 2 107 DiI MSC PBS 10 n=16 2 DiI 4 12 16 24 n=4 Image J MSC modified Pauli s score 24 OARSI score 100 MSC 14 50 MSC MSC MSC 2 DiI MSC - 2 - MSC 4 4 12 4 12 MSC (p , n=4) 16 24 MSC 4 12 16 MSC 24 MSC MSC modified Pauli s score MSC 24 MSC 1 2 2 OARSI MSC (p , n=4)

3 MSC MSC MSC MSC MSC MSC MSC ex vivo MSC 10 60 MSC MSC 10 DiI 2 DiI 4 DiI LacZ MSC 3 21 LacZ 28 LacZ DiI DiI MSC 4 16 24 MSC - 3 - 24 MSC 2 1 4 12 MSC 2 MSC MSC MSC - 4 - 4559 2 1 107 100 L 6 3

4 6 6 ( 1 )


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