Transcription of UHPLC用高耐圧ガードカートリッジカラム EXP R …
1 EXP R 100 MPa YMC-Triart m ColumnEluentFlow rateTemperatureDetectionInjectionSample: YMC-Triart C18 ( m, 12 nm) 100 X : acetonitrile/water (60/40): mL/min : 25 C: UV at 270 nm: 1 L: 1. Uracil ( mg/mL) 2. Methyl benzoate ( L/mL) 3. Naphthalene ( mg/mL) 4. Butyl benzoate ( L/mL)EXP R EXP R 3% NEW LF-0013 uhplc 1 2 3450100150mV N4=19,700 N4=19,200 EXP R ( , ) X (mm) ( )YMC-Triart C18( m, 12 nm) X 5TA12SP9-E5Q1CC X 5TA12SP9-E503CC XPCHUHPEXP R ( , mm ) EXP Optimize Technologies,Inc.
2 EXP R XRHTF-017,200 XRHTF-0525,000 EXP R ( ) 137 MPa (5 ) 46,00046,00038,000 ( ) min / http : ( 1 ) 137 MPa 100 MPa(3 ) YMC Co., m YMC-Triart m uhplc 100 MPa pH (%) ( m) (nm)Triart C18 - 12 Triart C8 - 12 Triart - 10 0123456 minmAU0102030405060U111104C12345630 X mL/minx 4x minmAU0102030405060U111104Gx 1/2x 1/4124635124635 YMC-Triart m 1. Hydrochlorothiazide2.
3 Valsartan3. Losartan potassium 4. Amlodipine besilate5. Atorvastatin calcium hydrate6. Candesartan cilexetil : YMC-Triart C8 ( m, 12 nm): A) 10 mM CH3 COONH4-CH3 COOH (pH ) B) acetonitrile: 30 C: UV at 254 nm: 2 L: Agilent 1200 SLColumnEluentTemperature Detection Injection SystemYMC-Triart m (P110728A)(P 110706A)(P 110707A)100X MPa100X MPa200 X m(=2 X m 100 X ) MPaPC=269PC=450PC=626 (Peak capacity) m 200 mm (2 ) 5 m PC (peak capacity)=1+ (gradient time / peak width*) *peak width = min112 minColumnEluent Flow rateTemperatureDetectionInjection Sample System : YMC-Triart C18 : A) water/TFA (100 ) B) acetonitrile/TFA (100 ) 5-40%B (0-15min) for a single column 5-40%B (0-30min) for two coupled columns.
4 ML/min: 70 C: UV at 220 nm: 10 L for a single column 20 L for two coupled columns: Triptic digest of Bovine Hemoglobin : Agilent 1290 uhplc 600-8106 284 YMC 4 FTEL.(075)342-4503 FAX.(075)342-4530 101-0032 2-6-2 3 FTEL.(03)5687-3131 FAX.(03)5687-313910%B ( min)10-90%B ( min)10%B ( min)10-90%B ( min)10%B ( min)10-90%B ( min) C18
