Version 1 - bioneer.co.kr

1 Version 1 Clinical Sample Processing Guide Introduction . ExiPrep Extraction Kit , ExiStation Extraction Kit AccuPrep Extraction Kit .. , .. Safety Warnings and Precautions BIONEER s Customer Service Center (MSDS) . , , .. ( ) . (tip, tube ) . 2 Clinical Sample Processing Guide [CONTENTS] 1. Processing 2. Processing 3. Processing 4. Processing 5. Processing 6. Processing Bacterial 7. Processing Viral 8. Processing 9. 3 Clinical Sample Processing Guide 1.

2 Processing Sputum : 1 : (7 ), (7 ) : Table top centrifuge (13,000 rpm ), vortex mixer, 10N NaOH, 1X PBS buffer (pH ~ ) Sputum sputum 1/10 volume 10 N NaOH 3 vortex (25 C) 15 incubation . ) NaOH 2 incubation 30 . NaOH conical tube PBS DEPC water vortex . 1 vortex microcentrifuge tube . 9,000 rpm 5 ) pellet cell pellet . 1X PBS buffer 1 30 vortex cell pellet washing 9,000 rpm 5 . (2~3 ) ) pellet cell pellet.

3 400 Resuspension Buffer 1x PBS, normal saline 6,000 rpm 5 spin-down . (ExiPrep 16 Dx: 400 / ExiPrep 16 : 200 ) 4 Clinical Sample Processing Guide 2 Processing Fluids : BAL, Pleural fluid, Saliva body fluid : 5 : (7 ), (7 ) : Table top centrifuge (13,000 rpm ), vortex mixer, 10N NaOH, 1X PBS buffer (pH ~ ) Fluid type tube vortex 5~10mL Conical tube . ) 10N NaOH 200 15 incubation . Fluid . 3,000rpm 20 1X PBS buffer 1 30 vortex microcentrifuge tube . 9,000 rpm 5 . ) pellet cell pellet 400 Resuspension Buffer 1x PBS, normal saline 6,000 rpm 5 spin-down.

4 (ExiPrep 16 Dx: 400 / ExiPrep 16 : 200 ) (Sample loading well or tube) 5 Clinical Sample Processing Guide 3 Processing Tissue : 50 ~ 100 : (7 ), (7 ) : Table top centrifuge (13,000 rpm ), vortex mixer, shaking incubator(60 C) Tissue 50 -100 microcentrifuge tube . 20 proteinase K (20 / , ), 400 Tissue lysis buffer ( ) 60 C shaking incubatior tissue lysis incubation . ) 1 , overnight incubation . 8,000 rpm 2 . (ExiPrep 16 Dx: 400 / ExiPrep 16 : 200 ) ) Pellet (Sample loading well or tube) 6 Clinical Sample Processing Guide 4 Processing FFPE(Formalin-Fixed Paraffin-Embedded Tissue) : 20 : : Table top centrifuge (13,000 rpm ), vortex mixer, shaking incubator (56 C, 60 C) FFPE 20 microcentrifuge tube.

5 1200ul xylene vortex 56 C 1 Heating . Paraffin vortex 13,000rpm/RT/5min . 1mL 100% ethanol vortexing . 13,000rpm/RT/5min 1mL 80% ethanol vortexing . 13,000rpm/RT/5min 1mL 60% ethanol vortexing . 13,000rpm/RT/5min . (Tube-Cap 10 ) 1X PBS buffer 1mL vortex 13,000rpm/RT/5min 20 proteinase K (20 / , ), 400 Tissue lysis buffer ( ) 60 C shaking incubatior tissue lysis incubation . ) 3 , overnight incubation . 8,000 rpm 2 . (ExiPrep 16 Dx: 400 / ExiPrep 16 : 200 ) ) Pellet . (Sample loading well or tube) 7 Clinical Sample Processing Guide 5 Processing Urine : 10 , : (7 ), (7 ) : Swing rotor centrifuge (3,000 rpm ), vortex mixer tube 3,000 rpm 20 400 Resuspension Buffer 1x PBS, normal saline.

6 (ExiPrep 16 Dx: 400 / ExiPrep 16 : 200 ) ) buffer 3 . (Sample loading well or tube) 8 Clinical Sample Processing Guide 6 Processing Bacterial Swabs : 1 brush ( brush ), Vaginal/Cervical ( brush ) 2 ( 1 ) , . : (7 ), (7 ) : Table top centrifuge (13,000 rpm ), vortex mixer, 1X PBS buffer (pH ~ ) tube 3 vortex 500 microcentrifuge tube ) Buffer swab . ) Tip PBS 2mL Vortex . 13,000 rpm 5 . 400 Resuspension Buffer 1x PBS, normal saline.

7 (ExiPrep 16 Dx: 400 / ExiPrep 16 : 200 ) ) Resuspension tip suspension pellet . (Sample loading well or tube) 9 Clinical Sample Processing Guide 7 Processing Viral Swabs : 1 brush 1 , . (Nasal swab ) swab ( 1/3) (Throat swab ) swab ( ). : (3 ), (3 ) : Table top centrifuge (13,000 rpm ), vortex mixer Swab virus 1x PBS, normal saline vortex mixer . microcentrifuge tube 500 6,000 rpm 5 .. (ExiPrep 16 Dx: 400 / ExiPrep 16 : 200 ) (Sample Loading well or tube) 10 Clinical Sample Processing Guide 8 Processing Stool : 1~2 g , , 1~2 g.

8 : (3 ), : Table top centrifuge (13,000 rpm ), vortex mixer, shaking incubator (60 C), PBS, Stool lysis buffer ( ), Proteinase K ( ) Stool for Virus 15 tube 1 g 9 PBS . Tube 3 vortexing . 3,000 rpm, 4 C 20 . (ExiPrep 16 Dx: 400 / ExiPrep 16 : 200 ) Stool for Bacteria tube 50~200 400 Stool lysis buffer 10 proteinase K (40 / ) . Tube 1 vortexing 60 C shaking incubator incubation . (30 ) lysis 12,000 rpm 5 . (ExiPrep 16 Dx: 400 / ExiPrep 16 : 200 ) 11 Clinical Sample Processing Guide 9 Index (Tuberculosis) Sputum Influenza/ Adenovirus Nasal Swab BAL Fluids Throat swab Tissue (STD) Urine (Pnuemonia) Sputum Swab Fluids Stool Norovirus Stool Swab Swab HSV Swab HPV Swab CSF Sputum (1 ) Tuberculosis, Pneumonia Page 3 Fluids (5 ) Bacteria - Tuberculosis, Pneumonia Page 4 Tissue (50 ~ 100 ) Tuberculosis, Pneumonia Page 5 FFPE (20 ) Tuberculosis Page 6 Urine (10 ) Tuberculosis, , , , , STI, etc.

9 Page 7 Swab (1 ) Bacteria , , , , STI, etc. Page 8 Virus Respiratory virus, Adenovirus, Norovirus, HSV, HPV Page 9 Stool (50 ~ 200 /200 ) Virus Norovirus, Enterovirus, Enterovirus 71 Page 10 Bacteria , , , , Y. enterocolitica, , , O157:H7 Page 10 Bioneer Worldwide Headquarters Address 8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon 306-220, Korea Tel +82-42-930-8777 (Korea only: 1588-9788) Fax +82-42-930-8600 E-mail Web Pangyo R&D Center Address #702, Building B, Korea Bio Park, 700, Daewangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-400 Tel +82-31-628-0500 (Korea only: 1588-9788) Fax +82-31-628-0555 E-mail Web Bioneer,Inc. Address 1000 Atlantic Avenue Alameda, CA 94501 USA Tel +1-877-264-4300 (Toll Free) Fax +1-510-865-0350 E-mail Web Bioneer Trade (Shanghai) Co, Ltd Address 403 Room, Building 88, , Zuchongzhi Road, Zhangjiang High Tech Park, PuDong New District, Shanghai 201203, China Tel +86-21-5080-0969 Fax +86-21-5080-1620 E-mail Web Copyright 2012 Bioneer Corporation.

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