遺伝子関連試薬 - wako-chem.jp

1 35 in situ TUNEL TdT -dUTP 3 OH DNA PDO POD-DAB Protein Digestion Enzyme TdT TdT Substrate Solution 100 POD-Conjugated Antibody DAB Solution DAB Enhancer DNaseI 10 DNaseIReaction Buffer 60,00040 in situ 298-60201 ( ) 1mL 40 L 44 L 200 L 4 L 40 L CHO-K1 400 400 DAB 200 T cell HE 200 B cell HE 200 200 36 ISHR Starting Kit in situhybridization ISH RNA ISH DNA ISH 75,00020 2 ISHR Starting Kit314-02611 ( )

2 In situhybridization RNA DNA 20 C NGF 1 g/ L 2 L 1 2 L 1 mol/L pH 800 L 1 Ethachinmate 2 10 C Ethachinmate 10 L 1 3 mol/L pH 100 L 1 2 10 C 4 RNA Synthesis Set 20 C 5 RNA Polymerase Buffer250 mmol/L NaCl10 mmol/L rATP10 mmol/L rGTP10 mmol/L rCTP10 mmol/L rUTPRNase lnhibitorSP6 RNA PolymeraseT7 RNA PolymeraseT3 RNA Polymerase DNaseI RT Grade H2O 20 2 in situhybridization 20 2 DIG ISHR 4 ISHR 1 ISHR 2 1 Noji, S. et al. : Nature, 350, 83 1991 2 Pardue, M. L. : In situ hybridization in Nucleic acid hybridization , IRL Press 1985 3 Noji, S., Yamaai, T., Koyama, E. and Taniguchi, S.

3 : Radioisotopes, 38, 366 1989 4 Noji, S., Takahashi, N., Nohno, T., Koyama, E., Yamaai, T., Muramatsu, M. and Taniguchi, S. : Acta. , 23, 353 1990 5 Kiyama, H., Emson, P. C. and Tohyama, M. : Neurosci. Res., 9, 1 1990 6 : In situ 1989 7 : , 7 13 1989 8 : in situ 1997 20 L 1 10 L 1 5 L 1 5 L 1 5 L 1 5 L 1 5 L 1 5 L 1 5 L 1 5 L 1 5 L 1 500 L 1 20 C10mg/mL RNase ARNase A Solution 2 ISHR 10 NaCI, 10mmol/L 2mg/mL GlycinePBS Glycine Buffer400mL 1 ISHR 2 Triethanolamine Acetylation Buffer800mL 1 ISHR 3 Acetic anhydrideAcetic anhydride1 mL 2 ISHR 4 NaCI, Sodium citrate4 SSC1L 2 ISHR 5 20 C10mg/mL Proteinase KProteinase K Solution200 L 1 ISHR 6 20 C50 Formamide, 2 SSC, 1 g/ L tRNA, 1 g/ L Salmon sperm DNA, 1 g/ L BSA, 10 Dextran sulfateHybridization Buffer93 L 8 ISHR 7 20 DTT Solution100 L 1 ISHR 8 NaCI, 10mmol/L Tris-HCI ,1mmol/L EDTANTE Buffer800mL 1 ISHR 9 NaCI, 10mmol/L PBS Buffer800mL 2 ISHR SSC600mL 2 15mmol/L NaCI, Sodium citrateISHR 11 37In situhybridization Reagents (ISHR)in situhybridization(ISH)

4 RNA ISH / RNA RNase mRNA RNA RNA ISH RNase 310-02831313-02821316-02811319-02801316- 02791319-02781312-02771315-02761318-0275 1311-02741 9,0001 LAcetylation BufferISHR 39,0001L4 SSCISHR 516,0005mLAcetic anhydrideISHR 49,0001 LPBS(Glycine) BufferISHR 29,0001 LPBS BufferISHR 121,000465 L 5 Hybridization BufferISHR 79,0001 LNTE BufferISHR 99,00093mgHybridization Buffer DTTISHR 821, 5 RNase A SolutionISHR SSC9,0001 LISHR 11 ( ) In situhybridization 20 5 ISHR RNA DIG RNA SP6 RNA Synthesis Set Code T3 RNA Synthesis Set Code T7 RNA Synthesis Set Code ISHR 4 130,0001kitIn situhybridization Reagents (ISHR)316-01951 ( ) 30,00020 307-135319,0005 CUGA RNA 304-1464113,500100mgProteinase K169-2104193,0001g165-210439,0005 CUGA in vitroTranscription Kit301-1549130,00020 307-15493 ( )

5 20 C10mg/mL RNase ARNase A Solution 5 ISHR 10 NaCI, 10mmol/L 2mg/mL GlycinePBS Glycine Buffer1L 1 ISHR 2 Triethanolamine Acetylation Buffer1L 2 ISHR 3 Acetic anhydrideAcetic anhydride1mL 5 ISHR 4 NaCI, Sodium citrate4 SSC1L 5 ISHR 52 10 CProteinase KProteinase K 5mg 1 ISHR 6 20 C50 Formamide, 2 SSC, 1 g/ L tRNA, 1 g/ L Salmon sperm DNA, 1 g/ L BSA, 10 Dextran sulfateHybridization Buffer465 L 5 ISHR 72 10 CDTT H2O 500 L Hybridization Buffer DTT 93mg 1 ISHR 8 NaCI, 10mmol/L Tris-HCI ,1mmol/L EDTANTE Buffer 1L 1 ISHR 9 NaCI, 10mmol/L PBS Buffer1L 4 ISHR SSC1L 3 15mmol/L NaCI, Sodium citrateISHR 11 38 25,0005L317-900119,000500mL 20 SSC319-9001513,00010mL 5mg/mL 049-271916,8001g049-217533,000250mg 043-2175123,6005g047-2175414,0001mL 10mg/mL 047-275117,00050mL 50 043-2187120,000100mg 015-2095155,0001mL 5043-27513 ( ) Kapak C C 150 C 205 CKAP(505)Kapak 10 12 KAP(504)Kapak 8 12 13,10040 537-1153113,10075 KAP(502)Kapak 8 530-1152113,10030 533-19171 ( ) SDS NaCl DNA RNA Cornish, E.

6 C., Beckham, S. A. and Maddox, J. F. : Biotecniques, 25, 947 (1998 13,000100mL 544-01331 ( ) 39 FISH(fluorescencein situhybridization) FISH FISH ES FISH 6 16 FISH Y X Y X FISH FISH FISH 300,000 50,000 DNA (100kb ) FISH 3 300,000 2 400,000 FISH 1 150,000 300,000 cDNA DNA ( kb ) ( ) 125,000 10 FISH (BAC PAC ) 50,000 10 FISH (DNA ) 80,000 10 (100 L) Y FISH (FITC Biotin )ROY-1054,000 10 (100 L) Y FISH (FITC Biotin )POY-10120,000 10 (100 L) XY FISH (Y :FITC, X :Biotin)CXY-1099,000 10 (100 L) XY FISH (Y :FITC, X :Biotin)MXY-1099,000 10 (100 L) XY FISH (Y :FITC, X.))

7 Biotin)HXY-10 ( ) 25,0001 / 5 G ( )CSC-0525,0001 / 5 G ( )CSR-0525,0001 / 5 G ( )CSM-0525,0001 / 5 G ( )CSH-05 ( ) 40 DNA PS DNA 2mL 34mg DNA DNA ,000100 DNA PS-Rapid 291-56401 ( ) A 90 C DNA DNA DNA 1 DNA DNA PCR DNA PS-Rapid DNA PS 20 DNA DNA 15 20 DNA 500bp DNA PCR DNA Isolation Solution 10mL 5 URL ,000100 DNA PS 295-52401 ( ) 3,700500mL168-216752,600100mL 2- 166-216711,850100mL 052-072214,250500mL054-07225 ( )

8 DNA -globin M X174/HaeIIILane 1 -globin 205 bp Lane 2 -globin 325 bp Lane 3 -globin 408 bp A 123 123A 123 123A 123 123A 123 123A 123 123A 123 123 MMMMMM